LOCUS       BBa_K1662002            1828 bp    DNA     linear       22-May-2024
DEFINITION  Construct 3 Fusion: TetR Promoter:PelB-CDA-eGFP
ACCESSION   BBa_K1662002
VERSION     BBa_K1662002.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=BBa_R0040 TetR Promoter 
     misc_feature    55..60
                     /label=scar
     RBS             88..102
                     /label=BBa_B0030 RBS
     misc_feature    110..112
                     /label=Start codon
     misc_feature    113..175
                     /label=BBa_J32015 PelB 
     misc_feature    176..979
                     /label=Mature CDA2
     misc_feature    980..1693
                     /label=eGFP
     misc_feature    1694..1699
                     /label=Double stop codon
     misc_feature    1700..1828
                     /label=BBa_B0015 terminator
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 atacgacagc acccgctgtg ttattcaatt aaagaggaga aagccgatta tgaaatacct
      121 gctgccgacc gctgctgctg gtctgctgct cctcgctgcc cagccggcga tggccgcagc
      181 tacaaaaacg ccttttccgg attggcttag tgcatttacc gggttaaaag aatggcctgg
      241 gttagatcca ccttatatac ctttagattt cattgatttc agtcaaattc cagattataa
      301 ggaatatgat caaaaccatt gcgacagtgt tccaagggac tcgtgctctt tcgattgcca
      361 tcactgcacc gaacacgatg atgtgtacac atgttccaaa ctttcccaga catttgacga
      421 tggtccttct gcttccacta ctaaattatt ggaccggttg aagcataatt ccaccttctt
      481 caatttaggt gtcaatatag ttcaacatcc agatatctat caaagaatgc aaaaggaggg
      541 acacttaatc ggctcacata cctggtctca cgtatatttg ccaaatgtat cgaatgaaaa
      601 aattatagct caaattgaat ggtccatctg ggcgatgaat gctactggca accatacccc
      661 caaatggttc agacctccat atggcggaat agataataga gtaagagcaa taacaaggca
      721 atttggctta caagccgtct tatgggatca cgatactttt gattggagcc tccttctcaa
      781 tgattctgtc ataactgaac aagaaattct tcaaaatgta ataaactgga acaagtcagg
      841 aaccggatta atattagaac acgattcaac ggaaaaaact gtcgatcttg ccattaagat
      901 aaataagttg ataggtgatg atcaatcaac agtttctcat tgtgtcggcg gaattgatta
      961 cataaaagag ttcttgtccg ctagcaaagg agaagaactc ttcactggag ttgtcccaat
     1021 tcttgttgaa ttagatggtg atgttaacgg ccacaagttc tctgtcagtg gagagggtga
     1081 aggtgatgca acatacggaa aacttaccct gaagttcatc tgcactactg gcaaactgcc
     1141 tgttccatgg ccaaccctgg tcactactct gtgctatggt gttcaatgct tttcaagata
     1201 cccggatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     1261 ggaaaggacc atcttcttca aagatgacgg caactacaag acacgtgctg aagtcaagtt
     1321 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgacttca aggaagatgg
     1381 caacattctg ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     1441 agacaaacaa aagaatggaa tcaaagtgaa cttcaagacc cgccacaaca ttgaagatgg
     1501 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     1561 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
     1621 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     1681 tgaactgtac aattaataac caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1741 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1801 ccttcgggtg ggcctttctg cgtttata
//