BBa_K1662002
1
BBa_K1662002
Construct 3 Fusion: TetR Promoter:PelB-CDA-eGFP
2015-09-03T11:00:00Z
2015-09-14T11:15:25Z
Mature CDA2 comes from saccharomyces cerevisiae. PelB sequence was pulled from the iGEM registry.
The purpose of this construct is to produce a single mRNA transcript that it can be translated into one fusion protein. This protein will be led into the periplasm of E. Coli because the PelB leader sequence leads the protein into the periplasm. Th PelB leader sequence leads the protein into the perpiplasm to keep the expression of the CDA gene from killing the E. Coli. The eGFP gene is there to allow the lab workers to see physically (green fluorescent light) that the mRNA transcript has been successfully translated and that the genes are being expressed correctly.
false
false
_2080_
28764
28764
9
false
Wobble introduced to eliminate illegal restriction sites.
false
Jack Kwon
annotation2453573
1
Start codon
range2453573
1
110
112
annotation2453577
1
Double stop codon
range2453577
1
1694
1699
annotation2453574
1
BBa_J32015 PelB
range2453574
1
113
175
annotation2453572
1
BBa_B0030 RBS
range2453572
1
88
102
annotation2453578
1
BBa_B0015 terminator
range2453578
1
1700
1828
annotation2453570
1
BBa_R0040 TetR Promoter
range2453570
1
1
54
annotation2453576
1
eGFP
range2453576
1
980
1693
annotation2453575
1
Mature CDA2
range2453575
1
176
979
annotation2453571
1
scar
range2453571
1
55
60
BBa_K1662002_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatacgacagcacccgctgtgttattcaattaaagaggagaaagccgattatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccgcagctacaaaaacgccttttccggattggcttagtgcatttaccgggttaaaagaatggcctgggttagatccaccttatatacctttagatttcattgatttcagtcaaattccagattataaggaatatgatcaaaaccattgcgacagtgttccaagggactcgtgctctttcgattgccatcactgcaccgaacacgatgatgtgtacacatgttccaaactttcccagacatttgacgatggtccttctgcttccactactaaattattggaccggttgaagcataattccaccttcttcaatttaggtgtcaatatagttcaacatccagatatctatcaaagaatgcaaaaggagggacacttaatcggctcacatacctggtctcacgtatatttgccaaatgtatcgaatgaaaaaattatagctcaaattgaatggtccatctgggcgatgaatgctactggcaaccatacccccaaatggttcagacctccatatggcggaatagataatagagtaagagcaataacaaggcaatttggcttacaagccgtcttatgggatcacgatacttttgattggagcctccttctcaatgattctgtcataactgaacaagaaattcttcaaaatgtaataaactggaacaagtcaggaaccggattaatattagaacacgattcaacggaaaaaactgtcgatcttgccattaagataaataagttgataggtgatgatcaatcaacagtttctcattgtgtcggcggaattgattacataaaagagttcttgtccgctagcaaaggagaagaactcttcactggagttgtcccaattcttgttgaattagatggtgatgttaacggccacaagttctctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctgaagttcatctgcactactggcaaactgcctgttccatggccaaccctggtcactactctgtgctatggtgttcaatgcttttcaagatacccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaaggaccatcttcttcaaagatgacggcaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgacttcaaggaagatggcaacattctgggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagtgaacttcaagacccgccacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactgtacaattaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z