BBa_K1662002 1 BBa_K1662002 Construct 3 Fusion: TetR Promoter:PelB-CDA-eGFP 2015-09-03T11:00:00Z 2015-09-14T11:15:25Z Mature CDA2 comes from saccharomyces cerevisiae. PelB sequence was pulled from the iGEM registry. The purpose of this construct is to produce a single mRNA transcript that it can be translated into one fusion protein. This protein will be led into the periplasm of E. Coli because the PelB leader sequence leads the protein into the periplasm. Th PelB leader sequence leads the protein into the perpiplasm to keep the expression of the CDA gene from killing the E. Coli. The eGFP gene is there to allow the lab workers to see physically (green fluorescent light) that the mRNA transcript has been successfully translated and that the genes are being expressed correctly. false false _2080_ 28764 28764 9 false Wobble introduced to eliminate illegal restriction sites. false Jack Kwon annotation2453573 1 Start codon range2453573 1 110 112 annotation2453577 1 Double stop codon range2453577 1 1694 1699 annotation2453574 1 BBa_J32015 PelB range2453574 1 113 175 annotation2453572 1 BBa_B0030 RBS range2453572 1 88 102 annotation2453578 1 BBa_B0015 terminator range2453578 1 1700 1828 annotation2453570 1 BBa_R0040 TetR Promoter range2453570 1 1 54 annotation2453576 1 eGFP range2453576 1 980 1693 annotation2453575 1 Mature CDA2 range2453575 1 176 979 annotation2453571 1 scar range2453571 1 55 60 BBa_K1662002_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatacgacagcacccgctgtgttattcaattaaagaggagaaagccgattatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccgcagctacaaaaacgccttttccggattggcttagtgcatttaccgggttaaaagaatggcctgggttagatccaccttatatacctttagatttcattgatttcagtcaaattccagattataaggaatatgatcaaaaccattgcgacagtgttccaagggactcgtgctctttcgattgccatcactgcaccgaacacgatgatgtgtacacatgttccaaactttcccagacatttgacgatggtccttctgcttccactactaaattattggaccggttgaagcataattccaccttcttcaatttaggtgtcaatatagttcaacatccagatatctatcaaagaatgcaaaaggagggacacttaatcggctcacatacctggtctcacgtatatttgccaaatgtatcgaatgaaaaaattatagctcaaattgaatggtccatctgggcgatgaatgctactggcaaccatacccccaaatggttcagacctccatatggcggaatagataatagagtaagagcaataacaaggcaatttggcttacaagccgtcttatgggatcacgatacttttgattggagcctccttctcaatgattctgtcataactgaacaagaaattcttcaaaatgtaataaactggaacaagtcaggaaccggattaatattagaacacgattcaacggaaaaaactgtcgatcttgccattaagataaataagttgataggtgatgatcaatcaacagtttctcattgtgtcggcggaattgattacataaaagagttcttgtccgctagcaaaggagaagaactcttcactggagttgtcccaattcttgttgaattagatggtgatgttaacggccacaagttctctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctgaagttcatctgcactactggcaaactgcctgttccatggccaaccctggtcactactctgtgctatggtgttcaatgcttttcaagatacccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaaggaccatcttcttcaaagatgacggcaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgacttcaaggaagatggcaacattctgggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagtgaacttcaagacccgccacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactgtacaattaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z