BBa_K1666000 1 BBa_K1666000 LsrA of LuxS/AI-2 signaling pathway in Salmonalla 2015-08-25T11:00:00Z 2015-08-30T07:46:12Z It comes from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2(Gene ID: 1255601) In a process called quorum sensing, bacteria communicate with one another using secreted chemical signaling molecules termed autoinducers. As the density of the bacterial population increases, so does the concentration of secreted autoinducer molecules. When the concentration of the autoinducer reaches a critical threshold, it diffuses back into the cell and activates or represses certain target genes. An autoinducer called AI-2 is generated by many species of Gram-negative and Gram-positive bacteria. In the LuxS/AI-2 signaling system of Salmonella enterica serovar Typhimurium, AI-2 response involves genes named lsr (LuxS regulated) that encode ATP binding cassette-type transporter. And LsrA is ATP-binding protein of the ABC transporter. In our project, we set this part under a nisA promoter for the final purpose of constructing an integrated AI-2 response pathway in our engineering bacteria. false false _2084_ 28478 28478 9 Not in stock false The lsrA gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR.We added restriction enzyme sites when designing primers,and the assembly is done through the use of restriction sites (cutting and ligating). false Heming Wang annotation2439289 1 LsrA range2439289 1 1 1536 BBa_K1666000_sequence 1 atgcaaatcagtcacaatactgcatcccctctgatttgtgtgcagaacatttataaaagttattccggcgtcgaagtactaaagggaattgactttactctgcatgcgggagaggtgcacgcattgcttggcggcaatggtgcgggtaaatcaacattaatgaagattattgccggtatagtcccgccagatggagggactatcgatattgctggtgtgcgttgcagtcatttaacgcctctgaaggcgcaccagtatggcatttacctggttccccaggagcctctgttatttccgagtttatctgtgcgggaaaatatcttgtttggcttgcagggacgtcaggcctccacggaaaaaatgcagcagctattaaaggcgatgggatgccaactcgatccggcgagcgctgcgggtacgcttgatgttgcagaccgccagatcgttgaaattatgcgcggcttgatgcgcgactcgcgaatcttaattcttgatgagcccacggcgtcgttaacgccagccgaaactgatcggttatttacgcgtctgcaagagttgctgaaaaagggtgtcggaattgtatttatttctcataagctaccagaaattagacagttagctcactgcgttagcgtgatgcgtgacggtaaaatcgcattattcggaaaaacgcatgacctttctaccgacgagattattcaagctatcaccccggcaacgcagggcgtcagtctttccgcgaatcaaaagttgtggctggaattgcctggcagccgcccgcagaacgaacgcggcgcgacagtattagcgctggagtcactgacgggcgaaggttttatgaatatcaaccttgaggtgcgggcaggcgaaatccttggtctggccgggttggtcggcgcgggacgcacagaactggctgaaacgctgtacggtattagaccggtcaatgcggggcggatgctgttcaatggtcaagaaattaacgccctgacaacccaacagcggttgcagctcggcctggtctatttgccggaagatcggcagtcatccgggctgtatcttgacgcttccctggcatggaatgtctgttcgctgacccacaaccaaaaaggattttggataaagccccagcgggataacgccacccttgaacgttaccaccgcgcgttaaatatcaaactcaataatgccgaacaggcggcgcgtactttatccggcggtaaccagcaaaaagtattgattgccaaatgcctggaggcctctccgcaattactgattgtcgatgaaccgacccgcggtgtcgatgtctccgcccgcagcgatatttatcagctgttgcgcagtatcgcgcaacaaaatgtcgcggtgctatttatttcctccgatctggaagagatagagcagatggccgatcgcgtatatgtcatgcaccagggggaactgggggggcctgcgttatgcggcgaggaaattaacgttgataccatcatgcacgttgcgtttggcgaacatggtgcgtcggaggcaacatgttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z