BBa_K1666002 1 BBa_K1666002 LsrC of LuxS/AI-2 signaling pathway in Salmonalla 2015-09-05T11:00:00Z 2015-09-13T04:06:04Z We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene LsrA(Gene ID: 1255602). Quorum sensing is a process of bacterial cell-to-cell communication involving the production and detection of extracellular signaling molecules called autoinducers. And autoinducer-2 (AI-2) has been proposed to serve as a 'universal signal' for interspecies communication. In the LuxS/AI-2 signaling system of Salmonella Typhimurium, AI-2 response involves ATP binding cassette transporter encoded by genes named Lsr (LuxS regulated). And LsrC is thetransmembrane protein which constructed the ABC transport. In our project, we set this protein-coding part under a nisA promoter and try to integrate them in the genome of Lactobacillus or Lactococcus for the final purpose of constructing an integrated AI-2 response pathway of Salmonella in the engineered bacteria. false false _2084_ 28478 23948 9 false The lsrC gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR. We added restriction enzyme sites when designing primers, and the assembly is done through the use of restriction sites (cutting and ligating). false Heming Wang annotation2445156 1 lsrC range2445156 1 1 1089 BBa_K1666002_sequence 1 atgttgaaattcatccaaaataaccgggaagcgacggcactgctggcaatagtctgtttattcgtgtttcctggcgcgctggatagtcagtatttgagcgtgcaaacgctgacaatggttttcagtagcgcgcaaattttgatgctgttggcgattggcgcgacgatggtaatgctcacccgcaatattgatgtatcggtgggcgcgacgatggtaatgctcacccgcaatattgatgtatcggtgggctcgacgacaggaatgtgcgcggtattgctgggagtgatgttaaacgccggctatagcctgccggtcgcctgcctggccacactaatattaggaattgtcgccggattttttaatggcgtactggttgcctggttgaagatacccgccattgtcgccactctgggaacgctgggcttgtatcgtgggatcatgctgctatggacaggggggaaatggattgaaggattacccgcaggcttaaagcaactctctgctccggtgtttctgggaatttccgcaatcggctggtttaccctggtgttagcgctgcttatggcctggctcctggcgaaaaccgcctttggccgcaatttttacgccaccggcgataacctccagggcgcccggcaattgggtgtccgtaccgagatggtacgcatcatggcattttcacttaatggcggtatggcggcattggctggaatcgtgtttgcctcgcagattggcttcattcccaatcaaaccggcacggggctggaaatgaaagccatcgcggcctgcgtattggggggaattagcctgttaggcgggtcaggcacggtcatcggcgctattctcggcgcttattttcttacgcaaatcgatagtgtgttagtgctgctgcgtatccccgcctggtggaacgattttattgctggcctggtattgttgggcgtactggtattcgatgggcggctgcgttgcgcattacaacgcaatctgcgccgccagaaatatgcccgttttatatcaccacccactccactacagacggaagcaaaaacgcacgcacaacagaataaaaacaaagaggtggcatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z