BBa_K1667004 1 BBa_K1667004 suicide gene ccdB 2015-09-06T11:00:00Z 2015-09-07T06:58:03Z The sequence of the ccdB gene in this part is from BB_K145151 and BB_K1010007 in igem. The gene of ccdB is a lethal gene. The expression of ccdB gene will lead to the death of the host cells. false false _2085_ 27665 27665 9 false There is a rbs sequence in front of the ccdB gene fragment to ensure the translation of it. false Jinyan Chu BBa_K1667003 1 BBa_K1667003 RecA promoter 2015-09-06T11:00:00Z 2015-09-07T08:36:43Z There is a DNA spacer fragment in front of the promoter sequence. A UV induced promoter. When the host cell is exposed in UV, the promoter will be actived, and the transcription of downstram gene will be started. false false _2085_ 27665 27665 9 false The sequence of the RecA promoter in this part is from BB_J22106 in igem. false Jinyan Chu BBa_K1667001 1 BBa_K1667001 strong promoter-RNA thermometer 2015-09-06T11:00:00Z 2015-09-07T06:30:26Z The sequence of the strong promoter in this part is from BB_J23106 in igem. The sequence of the RNA thermometer in this part is from BB_K115017 in igem. A strong promoter followed with a RNA thermometer sequence, which regulate the transcription of downstream gene in a temperature-dependent manner. When the temperature is above 32 degree centigrade, the gene transcription will go on, otherwise the gene transcription will be blocked. false false _2085_ 27665 27665 9 false This strong promoter is commonly used in gene engineering, and the RNA thermometer of 32 degree centigrade is very easy to control. false Jinyan Chu BBa_K1667005 1 BBa_K1667005 Thermo-regulated opdA Coupled with RecA-reduced ccdB 2015-09-06T11:00:00Z 2015-09-16T08:03:43Z The sequence of the strong promoter in this part is from BB_J23106 in igem. The sequence of the RNA thermometer in this part is from BB_K115017 in igem. The sequence of the opdA gene in this part is from BB_K215090, BB_K215091, and BB_K1010008 in igem. The sequence of the RecA（SOS）promoter in this part is from BB_J22106 in igem. The sequence of the ccdB gene in this part is from BB_K145151 and BB_K1010007 in igem. The sequence of the ompA in this part is from AJ617284.1 in GenBank. This part includes two individual DNA domains. 1. A strong promoter followed with a RNA thermometer sequence, which regulate the transcription of opdA gene and expression of opdA protein out of the host cell via an ompA peptide. This DNA fragment domain could be obtained by double-digested with EcoR I + Spe I. 2. A UV induced promoter (RecA SOS) followed with a suicide gene ccdB, which could lead to the death of host cell if exposed in UV. This DNA fragment domain could be obtained by double-digested with Xho I + Spe I. And the whole part DNA fragment could be obtained by double-digested with Xho I + EcoR I. false false _2085_ 27665 27665 9 false There is a DNA spacer fragment between the two domains in this parts. false Mingkai Xu component2445627 1 BBa_K1667004 component2445624 1 BBa_K1667001 component2445625 1 BBa_K1667002 component2445626 1 BBa_K1667003 annotation2445624 1 BBa_K1667001 range2445624 1 1 124 annotation2445626 1 BBa_K1667003 range2445626 1 1193 1431 annotation2445625 1 BBa_K1667002 range2445625 1 125 1192 annotation2445627 1 BBa_K1667004 range2445627 1 1432 1776 BBa_K1667002 1 BBa_K1667002 opdA coding sequence 2015-09-06T11:00:00Z 2015-09-07T06:42:38Z The sequence of the opdA gene in this part is from BB_K215090, BB_K215091, and BB_K1010008 in igem. A DNA sequence coding opdA protein which has the alility to degrade organophosphorus pesticide. false false _2085_ 27665 27665 9 false There is a ompA signal peptide in front of the opdA gene. The ompA piptide could lead the expressed opdA protein out of the host cells. false Jinyan Chu BBa_K1667001_sequence 1 tttacggctagctcagtcctaggtatagtgctagcccgggcgcccttcgggggcccggcggagacgggcgccggaggtgtccgacgcctgctcgtccagtctttgctcagtggaggattactag BBa_K1667002_sequence 1 atgaaaaaaaccgctatcgcgatcgcagttgcactggctggtttcgctaccgttgcgcaggccaagcttggcgatctgatcaataccgtgcgcggcccgatcccggtgagcgaagcgggttttaccctgacccatgaacatatctgcggtagcagcgccggctttctgcgcgcctggccggagttctttggtagccgtaaagccctggccgaaaaagccgtgcgcggtctgcgtcacgcgcgtgccgcgggcgttcagaccatcgtggatgtgagcaccttcgatattggccgcgatgttcgtctgctggccgaagttagccgtgccgcggatgttcatattgtggcggccaccggtctgtggtttgatccgccgctgagcatgcgcatgcgtagcgtggaagaactgacccagtttttcctgcgtgaaattcagcatggcattgaagataccggcattcgtgcgggtattatcaaagtggccaccaccggcaaagccaccccgtttcaggaactggtgctgaaagcggcggcccgcgcgagcctggcgaccggcgtgccggtgaccacccataccagcgcgagccagcgcgatggcgaacagcaggcggcgatttttgaaagcgaaggcctgagcccgagccgtgtgtgcattggtcatagcgatgataccgatgatctgagctatctgaccggtctggcggcgcgtggttatctggtgggcctggatcgcatgccgtatagcgccattggcctggaaggtaatgcgagcgcgctggcgctgtttggcacccgtagctggcagacccgtgcgctgctgattaaagccctgattgatcgtggttataaagatcgcattctggtgagccatgattggctgtttggctttagcagctatgttaccaacatcatggatgtgatggatcgtattaatccggatggtatggcgtttgtgccgctgcgcgtgattccgttcctgcgtgaaaaaggtgtgccgccggaaaccctggcgggtgttaccgttgcgaatccggcgcgttttctgagcccgaccggcagcctcgagtga BBa_K1667004_sequence 1 taattttgtttaactttaagaaggagatataccaagcttatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa BBa_K1667005_sequence 1 tttacggctagctcagtcctaggtatagtgctagcccgggcgcccttcgggggcccggcggagacgggcgccggaggtgtccgacgcctgctcgtccagtctttgctcagtggaggattactagatgaaaaaaaccgctatcgcgatcgcagttgcactggctggtttcgctaccgttgcgcaggccaagcttggcgatctgatcaataccgtgcgcggcccgatcccggtgagcgaagcgggttttaccctgacccatgaacatatctgcggtagcagcgccggctttctgcgcgcctggccggagttctttggtagccgtaaagccctggccgaaaaagccgtgcgcggtctgcgtcacgcgcgtgccgcgggcgttcagaccatcgtggatgtgagcaccttcgatattggccgcgatgttcgtctgctggccgaagttagccgtgccgcggatgttcatattgtggcggccaccggtctgtggtttgatccgccgctgagcatgcgcatgcgtagcgtggaagaactgacccagtttttcctgcgtgaaattcagcatggcattgaagataccggcattcgtgcgggtattatcaaagtggccaccaccggcaaagccaccccgtttcaggaactggtgctgaaagcggcggcccgcgcgagcctggcgaccggcgtgccggtgaccacccataccagcgcgagccagcgcgatggcgaacagcaggcggcgatttttgaaagcgaaggcctgagcccgagccgtgtgtgcattggtcatagcgatgataccgatgatctgagctatctgaccggtctggcggcgcgtggttatctggtgggcctggatcgcatgccgtatagcgccattggcctggaaggtaatgcgagcgcgctggcgctgtttggcacccgtagctggcagacccgtgcgctgctgattaaagccctgattgatcgtggttataaagatcgcattctggtgagccatgattggctgtttggctttagcagctatgttaccaacatcatggatgtgatggatcgtattaatccggatggtatggcgtttgtgccgctgcgcgtgattccgttcctgcgtgaaaaaggtgtgccgccggaaaccctggcgggtgttaccgttgcgaatccggcgcgttttctgagcccgaccggcagcctcgagtgacgaacagaaagtaatcgtattgtacacggccgcataatcgaactagaaacaatttctacaaaacacttgatactgtatgagcatacagtataattgcttcaacagaacatattgactatccggtattacccggcatgacaggagtaaaaatggctatcgacgaaaacaaacagaaagcgttggcggcagcactgggccagattgagaaacaatttggtaaaggctccatcatgtaataataattttgtttaactttaagaaggagatataccaagcttatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa BBa_K1667003_sequence 1 cgaacagaaagtaatcgtattgtacacggccgcataatcgaactagaaacaatttctacaaaacacttgatactgtatgagcatacagtataattgcttcaacagaacatattgactatccggtattacccggcatgacaggagtaaaaatggctatcgacgaaaacaaacagaaagcgttggcggcagcactgggccagattgagaaacaatttggtaaaggctccatcatgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z