LOCUS BBa_K1668009 3948 bp DNA linear 22-Nov-2024 DEFINITION plu0840-device ACCESSION BBa_K1668009 VERSION BBa_K1668009.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1215..1218 /label=conserved RBS 1211..1222 /label=BBa_B0034 CDS 1223..3079 /label=BBa_K1668006 misc_feature 3084..3087 /label=conserved RBS 3080..3091 /label=BBa_B0034 CDS 3098..3808 /label=mCherry misc_feature 3449..3449 /label=C->T (removing PstI site) misc_feature 3098..3811 /label=BBa_J06504 stem_loop 3831..3874 /label=stem_loop misc_feature 3820..3899 /label=BBa_B0010 terminator 3820..3899 /label=BBa_B0010 stem_loop 3915..3934 /label=T7 TE misc_feature 3941..3941 /label=stop polyA_site 3935..3948 /label=polya misc_feature 3908..3948 /label=BBa_B0012 terminator 3908..3948 /label=BBa_B0012 misc_feature 3080..3948 /label=BBa_J06702 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc aaagaggaga aaatgttgta taacacccca gtctgggcag atagctggct 1261 gttggacggt aacaattacc atgtaactgt tagcgacgaa cacagtaaag tgacacaatt 1321 aaaatttgaa aatcccaaaa accacgatca aatcattatc gacaatctgt ttgaaatcac 1381 gttgaaatca ggggaaaagc tcacggctaa agattttcag attactgata ttagcaaaaa 1441 gcacaatcgt attgttgtct ccctgaaaca tggcgacatt caggtaagaa atatcattac 1501 tgtggaggat aaagataagt acggctcgtt tgaaatcact attacaccgc aggaaaatgc 1561 gttggagctc agctctgtct cgttgatgcc atttcgcact aaaacccctt ttgttcacgg 1621 ttctatcgtc agttcaccga ttgtcagcga tagcttcttt attgttcccg gtaatccatt 1681 gattaccact gaggcgtata aagggagcgc ttcgcaaaaa attgcgctct ctatcccgtt 1741 agagccgggg aagacgttaa gttataagac gtatattggt gtttatcagg cagggcaatt 1801 gcggcgcaac gtcaatgagt ttttgaacaa tgctcgtccg cgtgaatata gtccttacct 1861 acattacaac tcttggtacg atattggtta cttcacgcct tataccgaac aagaggcgtt 1921 gacccgtatc aatcagtttg ggcaggagtt ggtgactaag cgcggtgtga agttgagtgg 1981 ttatctgttt gatgatggtt gggataatct caaaggagat tggggtttta gtcaggcatt 2041 tccccatgag ttcaaaaatc ttaaacaggc agcagatcaa tatcgtgctc gtttaggtat 2101 ttggctttct ccttggggcg gttatggtaa accgggtgag caacgtgttt ctcatgcgga 2161 ggagtttggt tatgaagtga tagatggcag atttgcgctc tctggcccta attactacgc 2221 caatttccat aagcgggtac tcaacttgat tcaacaacaa aatgtatcca tgtttaagct 2281 ggatggcacc ggcaatgctg atcgtgtgat taaagggagt tcattcacca gtgattttga 2341 tgccgctatc catctgattg acgatatgcg cagtacggaa agtgatctgt ttatcaactt 2401 gaccaccggc accaaagcca cgccttcctg gctgtttttc gccgattcta tttggcgtgg 2461 tggttatgat gtggagtttc acggcacagg ttctaaagtg cagcagtggc ttacctatcg 2521 tgatgcagaa acttatcgta atgtcgtaag aaatgggccg ctgttccctc ttaattcgtt 2581 gatgctgcat ggcattgttt atgccaaaca ggccaagcat ctggataaac agtcacctaa 2641 agattttgcc gatcaggttt ggagctattt tggcagcggc actcagttac aagagatgta 2701 tgttacgcct gacctgttga ccacagagga ttgggataca ctggcaaacg cggcgaaatg 2761 gtcagagaaa aataaagatg tgctgtttga tacccattgg attggtaaag atcctaccga 2821 tctagcggtg tatggttggg gagcctggag tccgaaaaag tcgattatta ctttgcgcaa 2881 tcctgctgat gaagtgaaac aatattatct ggatttgcaa agtgatctcg aattgcctga 2941 tgatcaacct tcggtatatg acgttaatat cgattatggc gagaacagct cgctgccgaa 3001 gcttatcaat aaaacggtgt tgattaccct tcaaccgtta gagacactga ccttttcttt 3061 gaccccatcg aaataataaa aagaggagaa atactagatg gtgagcaagg gcgaggagga 3121 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa 3181 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac 3241 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc 3301 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt 3361 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg 3421 cgtggtgacc gtgacccagg actcctcctt gcaggacggc gagttcatct acaaggtgaa 3481 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg 3541 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca 3601 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc 3661 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc 3721 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac 3781 cggcggcatg gacgagctgt acaagtaata atactagagc caggcatcaa ataaaacgaa 3841 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 3901 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //