BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_J06504
1
mCherry
monomeric RFP optimized for bacteria
2005-07-17T11:00:00Z
2016-01-25T01:05:28Z
mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
Released HQ 2013
mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
false
false
_20_
4206
340
20
In stock
false
<p>
Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR.
</p>
<p>
Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
</p>
true
ytwang
annotation1585829
1
mCherry
range1585829
1
1
711
annotation1585833
1
C->T (removing PstI site)
range1585833
1
352
352
BBa_K1668009
1
BBa_K1668009
plu0840-device
2015-09-07T11:00:00Z
2015-09-08T12:55:25Z
~
~
false
false
_2086_
24986
24510
9
false
~
false
Xintian Xu
component2447101
1
BBa_B0034
component2447099
1
BBa_I0500
component2447114
1
BBa_J06702
component2447102
1
BBa_K1668006
annotation2447099
1
BBa_I0500
range2447099
1
1
1210
annotation2447114
1
BBa_J06702
range2447114
1
3080
3948
annotation2447101
1
BBa_B0034
range2447101
1
1211
1222
annotation2447102
1
BBa_K1668006
range2447102
1
1223
3079
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1668006
1
BBa_K1668006
CDS <i>plu0840</i>
2015-09-07T11:00:00Z
2015-09-16T07:15:59Z
The metK gene was amplified by PCR with genomic DNA of S. avermitilis(具体型号) as template using primers metK1 and metK2
metK is the S-adenosylmethionine synthetase gene from Streptomyces avermitilis, which was found to stimulate the production of avermectins. When wild-type strain ATCC31267 was transformed with pYJ02 and pYJ03, two metK expression plasmids, avermectin production was increased about 2.0-fold and 5.5-fold compared with that in the control strains, respectively. The avermectin productivity of each culture was quantitatively measured by HPLC analysis.
As the kinetic study revealed, avermectin overproduction in recombinant strain was not caused by the change of cell growth rate or copy number effect. Instead, metK stimulates the avermectin production by increasing the intracellular concentration of S-adenosylmethionine (SAM), an important intermediate product in avermectin production. However, there may be a maximum concentration of SAM for the production of avermectin in S. avermitilis, over which it has no any effect on the antibiotic production.
The results of experiments showed that there were different effects of metK expression levels on avermectin production in various S. avermitilis strains. The gene expression levels of metK in two engineered strain, GB-165 and 76-05, were much higher then those in wild-type strain, whereas the avermectin productivity in these two strains were not significantly improved. It probably because the high expression level of metK limited the increasement of avermectin by overexpression of metK.
false
false
_2086_
24986
24510
9
false
We used seamless assembly.
false
Xintian Xu
BBa_J06702
1
mCherry
mCherry, bacterial with RBS and forward terminator
2005-07-24T11:00:00Z
2015-08-31T04:08:18Z
Released HQ 2013
Combines BBa_B0015 forward terminator with BBa_J06602 mCherry, bacterial with RBS
false
false
_20_
0
340
20
In stock
false
true
Yves Wang
component1596419
1
BBa_B0034
component1596432
1
BBa_B0010
component1596427
1
BBa_J06504
component1596442
1
BBa_B0012
annotation1596427
1
BBa_J06504
range1596427
1
19
732
annotation1596419
1
BBa_B0034
range1596419
1
1
12
annotation1596432
1
BBa_B0010
range1596432
1
741
820
annotation1596442
1
BBa_B0012
range1596442
1
829
869
BBa_I0500
1
pBad/araC
Inducible pBad/araC promoter
2003-12-05T12:00:00Z
2015-08-31T04:07:29Z
-- No description --
false
true
_1_
0
24
7
In stock
false
true
Sri Kosuri
annotation1498841
1
Pbad
range1498841
1
1080
1210
annotation1498843
1
AraC
range1498843
1
1
1079
annotation1498842
1
PC
range1498842
1
1043
1114
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_J06504_sequence
1
atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
BBa_K1668006_sequence
1
atgttgtataacaccccagtctgggcagatagctggctgttggacggtaacaattaccatgtaactgttagcgacgaacacagtaaagtgacacaattaaaatttgaaaatcccaaaaaccacgatcaaatcattatcgacaatctgtttgaaatcacgttgaaatcaggggaaaagctcacggctaaagattttcagattactgatattagcaaaaagcacaatcgtattgttgtctccctgaaacatggcgacattcaggtaagaaatatcattactgtggaggataaagataagtacggctcgtttgaaatcactattacaccgcaggaaaatgcgttggagctcagctctgtctcgttgatgccatttcgcactaaaaccccttttgttcacggttctatcgtcagttcaccgattgtcagcgatagcttctttattgttcccggtaatccattgattaccactgaggcgtataaagggagcgcttcgcaaaaaattgcgctctctatcccgttagagccggggaagacgttaagttataagacgtatattggtgtttatcaggcagggcaattgcggcgcaacgtcaatgagtttttgaacaatgctcgtccgcgtgaatatagtccttacctacattacaactcttggtacgatattggttacttcacgccttataccgaacaagaggcgttgacccgtatcaatcagtttgggcaggagttggtgactaagcgcggtgtgaagttgagtggttatctgtttgatgatggttgggataatctcaaaggagattggggttttagtcaggcatttccccatgagttcaaaaatcttaaacaggcagcagatcaatatcgtgctcgtttaggtatttggctttctccttggggcggttatggtaaaccgggtgagcaacgtgtttctcatgcggaggagtttggttatgaagtgatagatggcagatttgcgctctctggccctaattactacgccaatttccataagcgggtactcaacttgattcaacaacaaaatgtatccatgtttaagctggatggcaccggcaatgctgatcgtgtgattaaagggagttcattcaccagtgattttgatgccgctatccatctgattgacgatatgcgcagtacggaaagtgatctgtttatcaacttgaccaccggcaccaaagccacgccttcctggctgtttttcgccgattctatttggcgtggtggttatgatgtggagtttcacggcacaggttctaaagtgcagcagtggcttacctatcgtgatgcagaaacttatcgtaatgtcgtaagaaatgggccgctgttccctcttaattcgttgatgctgcatggcattgtttatgccaaacaggccaagcatctggataaacagtcacctaaagattttgccgatcaggtttggagctattttggcagcggcactcagttacaagagatgtatgttacgcctgacctgttgaccacagaggattgggatacactggcaaacgcggcgaaatggtcagagaaaaataaagatgtgctgtttgatacccattggattggtaaagatcctaccgatctagcggtgtatggttggggagcctggagtccgaaaaagtcgattattactttgcgcaatcctgctgatgaagtgaaacaatattatctggatttgcaaagtgatctcgaattgcctgatgatcaaccttcggtatatgacgttaatatcgattatggcgagaacagctcgctgccgaagcttatcaataaaacggtgttgattacccttcaaccgttagagacactgaccttttctttgaccccatcgaaataataa
BBa_I0500_sequence
1
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
BBa_K1668009_sequence
1
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcaaagaggagaaaatgttgtataacaccccagtctgggcagatagctggctgttggacggtaacaattaccatgtaactgttagcgacgaacacagtaaagtgacacaattaaaatttgaaaatcccaaaaaccacgatcaaatcattatcgacaatctgtttgaaatcacgttgaaatcaggggaaaagctcacggctaaagattttcagattactgatattagcaaaaagcacaatcgtattgttgtctccctgaaacatggcgacattcaggtaagaaatatcattactgtggaggataaagataagtacggctcgtttgaaatcactattacaccgcaggaaaatgcgttggagctcagctctgtctcgttgatgccatttcgcactaaaaccccttttgttcacggttctatcgtcagttcaccgattgtcagcgatagcttctttattgttcccggtaatccattgattaccactgaggcgtataaagggagcgcttcgcaaaaaattgcgctctctatcccgttagagccggggaagacgttaagttataagacgtatattggtgtttatcaggcagggcaattgcggcgcaacgtcaatgagtttttgaacaatgctcgtccgcgtgaatatagtccttacctacattacaactcttggtacgatattggttacttcacgccttataccgaacaagaggcgttgacccgtatcaatcagtttgggcaggagttggtgactaagcgcggtgtgaagttgagtggttatctgtttgatgatggttgggataatctcaaaggagattggggttttagtcaggcatttccccatgagttcaaaaatcttaaacaggcagcagatcaatatcgtgctcgtttaggtatttggctttctccttggggcggttatggtaaaccgggtgagcaacgtgtttctcatgcggaggagtttggttatgaagtgatagatggcagatttgcgctctctggccctaattactacgccaatttccataagcgggtactcaacttgattcaacaacaaaatgtatccatgtttaagctggatggcaccggcaatgctgatcgtgtgattaaagggagttcattcaccagtgattttgatgccgctatccatctgattgacgatatgcgcagtacggaaagtgatctgtttatcaacttgaccaccggcaccaaagccacgccttcctggctgtttttcgccgattctatttggcgtggtggttatgatgtggagtttcacggcacaggttctaaagtgcagcagtggcttacctatcgtgatgcagaaacttatcgtaatgtcgtaagaaatgggccgctgttccctcttaattcgttgatgctgcatggcattgtttatgccaaacaggccaagcatctggataaacagtcacctaaagattttgccgatcaggtttggagctattttggcagcggcactcagttacaagagatgtatgttacgcctgacctgttgaccacagaggattgggatacactggcaaacgcggcgaaatggtcagagaaaaataaagatgtgctgtttgatacccattggattggtaaagatcctaccgatctagcggtgtatggttggggagcctggagtccgaaaaagtcgattattactttgcgcaatcctgctgatgaagtgaaacaatattatctggatttgcaaagtgatctcgaattgcctgatgatcaaccttcggtatatgacgttaatatcgattatggcgagaacagctcgctgccgaagcttatcaataaaacggtgttgattacccttcaaccgttagagacactgaccttttctttgaccccatcgaaataataaaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J06702_sequence
1
aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z