BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_J06504 1 mCherry monomeric RFP optimized for bacteria 2005-07-17T11:00:00Z 2016-01-25T01:05:28Z mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible Released HQ 2013 mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends. false false _20_ 4206 340 20 In stock false <p> Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR. </p> <p> Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick. </p> true ytwang annotation1585829 1 mCherry range1585829 1 1 711 annotation1585833 1 C->T (removing PstI site) range1585833 1 352 352 BBa_K1668009 1 BBa_K1668009 plu0840-device 2015-09-07T11:00:00Z 2015-09-08T12:55:25Z ~ ~ false false _2086_ 24986 24510 9 false ~ false Xintian Xu component2447101 1 BBa_B0034 component2447099 1 BBa_I0500 component2447114 1 BBa_J06702 component2447102 1 BBa_K1668006 annotation2447099 1 BBa_I0500 range2447099 1 1 1210 annotation2447114 1 BBa_J06702 range2447114 1 3080 3948 annotation2447101 1 BBa_B0034 range2447101 1 1211 1222 annotation2447102 1 BBa_K1668006 range2447102 1 1223 3079 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1668006 1 BBa_K1668006 CDS <i>plu0840</i> 2015-09-07T11:00:00Z 2015-09-16T07:15:59Z The metK gene was amplified by PCR with genomic DNA of S. avermitilis(&#20855;&#20307;&#22411;&#21495;) as template using primers metK1 and metK2 metK is the S-adenosylmethionine synthetase gene from Streptomyces avermitilis, which was found to stimulate the production of avermectins. When wild-type strain ATCC31267 was transformed with pYJ02 and pYJ03, two metK expression plasmids, avermectin production was increased about 2.0-fold and 5.5-fold compared with that in the control strains, respectively. The avermectin productivity of each culture was quantitatively measured by HPLC analysis. As the kinetic study revealed, avermectin overproduction in recombinant strain was not caused by the change of cell growth rate or copy number effect. Instead, metK stimulates the avermectin production by increasing the intracellular concentration of S-adenosylmethionine (SAM), an important intermediate product in avermectin production. However, there may be a maximum concentration of SAM for the production of avermectin in S. avermitilis, over which it has no any effect on the antibiotic production. The results of experiments showed that there were different effects of metK expression levels on avermectin production in various S. avermitilis strains. The gene expression levels of metK in two engineered strain, GB-165 and 76-05, were much higher then those in wild-type strain, whereas the avermectin productivity in these two strains were not significantly improved. It probably because the high expression level of metK limited the increasement of avermectin by overexpression of metK. false false _2086_ 24986 24510 9 false We used seamless assembly. false Xintian Xu BBa_J06702 1 mCherry mCherry, bacterial with RBS and forward terminator 2005-07-24T11:00:00Z 2015-08-31T04:08:18Z Released HQ 2013 Combines BBa_B0015 forward terminator with BBa_J06602 mCherry, bacterial with RBS false false _20_ 0 340 20 In stock false true Yves Wang component1596419 1 BBa_B0034 component1596432 1 BBa_B0010 component1596427 1 BBa_J06504 component1596442 1 BBa_B0012 annotation1596427 1 BBa_J06504 range1596427 1 19 732 annotation1596419 1 BBa_B0034 range1596419 1 1 12 annotation1596432 1 BBa_B0010 range1596432 1 741 820 annotation1596442 1 BBa_B0012 range1596442 1 829 869 BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498841 1 Pbad range1498841 1 1080 1210 annotation1498843 1 AraC range1498843 1 1 1079 annotation1498842 1 PC range1498842 1 1043 1114 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_J06504_sequence 1 atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa BBa_K1668006_sequence 1 atgttgtataacaccccagtctgggcagatagctggctgttggacggtaacaattaccatgtaactgttagcgacgaacacagtaaagtgacacaattaaaatttgaaaatcccaaaaaccacgatcaaatcattatcgacaatctgtttgaaatcacgttgaaatcaggggaaaagctcacggctaaagattttcagattactgatattagcaaaaagcacaatcgtattgttgtctccctgaaacatggcgacattcaggtaagaaatatcattactgtggaggataaagataagtacggctcgtttgaaatcactattacaccgcaggaaaatgcgttggagctcagctctgtctcgttgatgccatttcgcactaaaaccccttttgttcacggttctatcgtcagttcaccgattgtcagcgatagcttctttattgttcccggtaatccattgattaccactgaggcgtataaagggagcgcttcgcaaaaaattgcgctctctatcccgttagagccggggaagacgttaagttataagacgtatattggtgtttatcaggcagggcaattgcggcgcaacgtcaatgagtttttgaacaatgctcgtccgcgtgaatatagtccttacctacattacaactcttggtacgatattggttacttcacgccttataccgaacaagaggcgttgacccgtatcaatcagtttgggcaggagttggtgactaagcgcggtgtgaagttgagtggttatctgtttgatgatggttgggataatctcaaaggagattggggttttagtcaggcatttccccatgagttcaaaaatcttaaacaggcagcagatcaatatcgtgctcgtttaggtatttggctttctccttggggcggttatggtaaaccgggtgagcaacgtgtttctcatgcggaggagtttggttatgaagtgatagatggcagatttgcgctctctggccctaattactacgccaatttccataagcgggtactcaacttgattcaacaacaaaatgtatccatgtttaagctggatggcaccggcaatgctgatcgtgtgattaaagggagttcattcaccagtgattttgatgccgctatccatctgattgacgatatgcgcagtacggaaagtgatctgtttatcaacttgaccaccggcaccaaagccacgccttcctggctgtttttcgccgattctatttggcgtggtggttatgatgtggagtttcacggcacaggttctaaagtgcagcagtggcttacctatcgtgatgcagaaacttatcgtaatgtcgtaagaaatgggccgctgttccctcttaattcgttgatgctgcatggcattgtttatgccaaacaggccaagcatctggataaacagtcacctaaagattttgccgatcaggtttggagctattttggcagcggcactcagttacaagagatgtatgttacgcctgacctgttgaccacagaggattgggatacactggcaaacgcggcgaaatggtcagagaaaaataaagatgtgctgtttgatacccattggattggtaaagatcctaccgatctagcggtgtatggttggggagcctggagtccgaaaaagtcgattattactttgcgcaatcctgctgatgaagtgaaacaatattatctggatttgcaaagtgatctcgaattgcctgatgatcaaccttcggtatatgacgttaatatcgattatggcgagaacagctcgctgccgaagcttatcaataaaacggtgttgattacccttcaaccgttagagacactgaccttttctttgaccccatcgaaataataa BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_K1668009_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcaaagaggagaaaatgttgtataacaccccagtctgggcagatagctggctgttggacggtaacaattaccatgtaactgttagcgacgaacacagtaaagtgacacaattaaaatttgaaaatcccaaaaaccacgatcaaatcattatcgacaatctgtttgaaatcacgttgaaatcaggggaaaagctcacggctaaagattttcagattactgatattagcaaaaagcacaatcgtattgttgtctccctgaaacatggcgacattcaggtaagaaatatcattactgtggaggataaagataagtacggctcgtttgaaatcactattacaccgcaggaaaatgcgttggagctcagctctgtctcgttgatgccatttcgcactaaaaccccttttgttcacggttctatcgtcagttcaccgattgtcagcgatagcttctttattgttcccggtaatccattgattaccactgaggcgtataaagggagcgcttcgcaaaaaattgcgctctctatcccgttagagccggggaagacgttaagttataagacgtatattggtgtttatcaggcagggcaattgcggcgcaacgtcaatgagtttttgaacaatgctcgtccgcgtgaatatagtccttacctacattacaactcttggtacgatattggttacttcacgccttataccgaacaagaggcgttgacccgtatcaatcagtttgggcaggagttggtgactaagcgcggtgtgaagttgagtggttatctgtttgatgatggttgggataatctcaaaggagattggggttttagtcaggcatttccccatgagttcaaaaatcttaaacaggcagcagatcaatatcgtgctcgtttaggtatttggctttctccttggggcggttatggtaaaccgggtgagcaacgtgtttctcatgcggaggagtttggttatgaagtgatagatggcagatttgcgctctctggccctaattactacgccaatttccataagcgggtactcaacttgattcaacaacaaaatgtatccatgtttaagctggatggcaccggcaatgctgatcgtgtgattaaagggagttcattcaccagtgattttgatgccgctatccatctgattgacgatatgcgcagtacggaaagtgatctgtttatcaacttgaccaccggcaccaaagccacgccttcctggctgtttttcgccgattctatttggcgtggtggttatgatgtggagtttcacggcacaggttctaaagtgcagcagtggcttacctatcgtgatgcagaaacttatcgtaatgtcgtaagaaatgggccgctgttccctcttaattcgttgatgctgcatggcattgtttatgccaaacaggccaagcatctggataaacagtcacctaaagattttgccgatcaggtttggagctattttggcagcggcactcagttacaagagatgtatgttacgcctgacctgttgaccacagaggattgggatacactggcaaacgcggcgaaatggtcagagaaaaataaagatgtgctgtttgatacccattggattggtaaagatcctaccgatctagcggtgtatggttggggagcctggagtccgaaaaagtcgattattactttgcgcaatcctgctgatgaagtgaaacaatattatctggatttgcaaagtgatctcgaattgcctgatgatcaaccttcggtatatgacgttaatatcgattatggcgagaacagctcgctgccgaagcttatcaataaaacggtgttgattacccttcaaccgttagagacactgaccttttctttgaccccatcgaaataataaaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J06702_sequence 1 aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z