LOCUS       BBa_K1668010            2503 bp    DNA     linear       23-May-2024
DEFINITION  plu1537-device
ACCESSION   BBa_K1668010
VERSION     BBa_K1668010.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1215..1218
                     /label=conserved
     RBS             1211..1222
                     /label=BBa_B0034
     CDS             1223..1634
                     /label=BBa_K1668007
     misc_feature    1639..1642
                     /label=conserved
     RBS             1635..1646
                     /label=BBa_B0034
     CDS             1653..2363
                     /label=mCherry
     misc_feature    2004..2004
                     /label=C->T (removing PstI site)
     misc_feature    1653..2366
                     /label=BBa_J06504
     stem_loop       2386..2429
                     /label=stem_loop
     misc_feature    2375..2454
                     /label=BBa_B0010
     terminator      2375..2454
                     /label=BBa_B0010
     stem_loop       2470..2489
                     /label=T7 TE
     misc_feature    2496..2496
                     /label=stop
     polyA_site      2490..2503
                     /label=polya
     misc_feature    2463..2503
                     /label=BBa_B0012
     terminator      2463..2503
                     /label=BBa_B0012
     misc_feature    1635..2503
                     /label=BBa_J06702
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc aaagaggaga aaaatgtcag agatcgaagt aatttcttta aaagcaccaa
     1261 ttagcgttat tgcaaaagta actaacaaat caccatataa acttaaactt atccaggatt
     1321 ctattcgtct ggatcaaggt gagtggacta ctcttcctcc tcaagtcatt aatagaagca
     1381 atgatagcac acctggaaaa gcgatttgga gaagtgattc aaacagcctg ataggtggtg
     1441 ttgcagggcg ctgtacttat gtatttgtgg atagcaaagg cgaaatctat agtatctata
     1501 ttacatggag taaccctttg attggcagca gcagctattc aatctcaacc gattatttag
     1561 gtgatgattt acatttatcc tatacagcag atggcggtaa taatccagta gtagaatata
     1621 tgattgtaag ctaaaaagag gagaaatact agatggtgag caagggcgag gaggataaca
     1681 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     1741 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     1801 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     1861 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     1921 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     1981 tgaccgtgac ccaggactcc tccttgcagg acggcgagtt catctacaag gtgaagctgc
     2041 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     2101 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     2161 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     2221 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     2281 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     2341 gcatggacga gctgtacaag taataatact agagccaggc atcaaataaa acgaaaggct
     2401 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     2461 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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