BBa_K1669000 1 BBa_K1669000 CtfA + His-tag 2015-09-11T11:00:00Z 2015-09-13T01:22:29Z Sequence retrieved from GenBank and codon optimized for expression in E. coli. This part includes the CtfA gene from Clostridium acetobutylicum (NCBI: CAP0163) with a C-terminal His-tag, which has been codon optimized for expression in E. coli. This is one of the two polipeptide chains forming the CtfAB (CoA-transferase) enzyme, that is in Clostridium acetobutylicum responsible for conversion of butyrate to butyril-CoA. The sequence contains a C-terminal His-tag for purification of the protein using the mmobilized-metal affinity chromatography and detected with the anti-His antibodyes. false false _2087_ 26210 27546 9 false C-terminal His-tag. false Marina Klemencic BBa_K1669000_sequence 1 atgaatagcaaaattattcgtttcgaaaacctgcgtagctttttcaaggatgggatgaccattatgatcggggggttcttaaattgtgggacaccaaccaaactgattgattttctcgtgaacctgaatattaaaaacttgacaattatcagcaacgacacctgctaccctaacaccggtatcggcaaactgatctcaaataaccaggtgaaaaaattgattgcctcctacatcggctccaatccggatactggcaaaaaactgttcaataacgaacttgaggttgaactgagcccccaaggtacccttgtggaacgtattcgtgcggggggttccggtttaggtggtgttttaacgaaaacgggtttgggaacactgatcgaaaaaggtaagaaaaagatcagtatcaatgggaccgaataccttttagaattgccactgactgccgatgttgcactcattaagggcagcatcgtggatgaagcaggcaatacattctataaaggcactaccaaaaactttaacccctatatggctatggccgcaaaaaccgtgattgttgaagccgagaatctcgtgtcttgtgaaaaactggaaaaagaaaaggccatgaccccgggtgtcttgattaattacatcgtaaaagagccggcacatcaccatcatcaccattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z