LOCUS       BBa_K1669002             781 bp    DNA     linear       26-Nov-2024
DEFINITION  CtfA with double terminator
ACCESSION   BBa_K1669002
VERSION     BBa_K1669002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=ATG
     misc_feature    669..673
                     /label=double stop codon
     misc_feature    650..668
                     /label=His-tag
     CDS             1..649
                     /label=CtfA
     CDS             1..678
                     /label=BBa_K1669001
     stem_loop       694..713
                     /label=T7 TE
     misc_feature    720..720
                     /label=stop
     polyA_site      714..727
                     /label=polya
     misc_feature    687..727
                     /label=BBa_B0012
     terminator      687..727
                     /label=BBa_B0012
     stem_loop       748..770
                     /label=stem_loop
     misc_feature    736..781
                     /label=BBa_B0011
     terminator      736..781
                     /label=BBa_B0011
     terminator      687..781
                     /label=BBa_B0014
     terminator      687..781
                     /label=BBa_K823017
ORIGIN
        1 atgaatagca aaattattcg tttcgaaaac ctgcgtagct ttttcaagga tgggatgacc
       61 attatgatcg gggggttctt aaattgtggg acaccaacca aactgattga ttttctcgtg
      121 aacctgaata ttaaaaactt gacaattatc agcaacgaca cctgctaccc taacaccggt
      181 atcggcaaac tgatctcaaa taaccaggtg aaaaaattga ttgcctccta catcggctcc
      241 aatccggata ctggcaaaaa actgttcaat aacgaacttg aggttgaact gagcccccaa
      301 ggtacccttg tggaacgtat tcgtgcgggg ggttccggtt taggtggtgt tttaacgaaa
      361 acgggtttgg gaacactgat cgaaaaaggt aagaaaaaga tcagtatcaa tgggaccgaa
      421 taccttttag aattgccact gactgccgat gttgcactca ttaagggcag catcgtggat
      481 gaagcaggca atacattcta taaaggcact accaaaaact ttaaccccta tatggctatg
      541 gccgcaaaaa ccgtgattgt tgaagccgag aatctcgtgt cttgtgaaaa actggaaaaa
      601 gaaaaggcca tgaccccggg tgtcttgatt aattacatcg taaaagagcc ggcacatcac
      661 catcatcacc attaataata ctagagtcac actggctcac cttcgggtgg gcctttctgc
      721 gtttatatac tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt
      781 t
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