BBa_K1669001
1
BBa_K1669001
CtfA + His-tag
2015-09-11T11:00:00Z
2015-09-12T01:47:27Z
Sequence retrieved from GenBank and codon optimized for expression in E. coli.
This part includes the CtfA gene from Clostridium acetobutylicum (NCBI: CAP0163) with a C-terminal His-tag, which has been codon optimized for expression in E. coli.
This is one of the two polipeptide chains forming the CtfAB (CoA-transferase) enzyme, that is in Clostridium acetobutylicum responsible for conversion of butyrate to butyril-CoA.
The sequence contains a C-terminal His-tag for purification of the protein using the mmobilized-metal affinity chromatography and detected with the anti-His antibodyes.
false
false
_2087_
27546
27546
9
false
Codon optimization for expression in E. coli.
Added C-terminal His-tag.
false
Marina Klemencic
annotation2452398
1
ATG
range2452398
1
1
3
annotation2452401
1
CtfA
range2452401
1
1
649
annotation2452400
1
His-tag
range2452400
1
650
668
annotation2452399
1
double stop codon
range2452399
1
669
673
BBa_K823017
1
BBa_K823017
double terminator (B0012-B0011)
2012-09-10T11:00:00Z
2015-05-08T01:13:30Z
Part:BBa_B0014
Released HQ 2013
This is a copy of the [[Part:B0014|Part:B0014]]. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3.
false
false
_1081_
0
11555
9
In stock
false
This is a copy of the [[Part:B0014|Part:B0014]]. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3.
false
Jara Radeck
component2182657
1
BBa_B0014
annotation2182657
1
BBa_B0014
range2182657
1
1
95
BBa_K1669002
1
BBa_K1669002
CtfA with double terminator
2015-09-11T11:00:00Z
2015-09-12T02:57:38Z
/
CtfA gene fused with double terminator.
false
false
_2087_
27546
27546
9
false
/
false
Marina Klemencic
component2452463
1
BBa_K1669001
component2452471
1
BBa_K823017
annotation2452471
1
BBa_K823017
range2452471
1
687
781
annotation2452463
1
BBa_K1669001
range2452463
1
1
678
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939303
1
BBa_B0012
component939311
1
BBa_B0011
annotation939311
1
BBa_B0011
range939311
1
50
95
annotation939303
1
BBa_B0012
range939303
1
1
41
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_K1669001_sequence
1
atgaatagcaaaattattcgtttcgaaaacctgcgtagctttttcaaggatgggatgaccattatgatcggggggttcttaaattgtgggacaccaaccaaactgattgattttctcgtgaacctgaatattaaaaacttgacaattatcagcaacgacacctgctaccctaacaccggtatcggcaaactgatctcaaataaccaggtgaaaaaattgattgcctcctacatcggctccaatccggatactggcaaaaaactgttcaataacgaacttgaggttgaactgagcccccaaggtacccttgtggaacgtattcgtgcggggggttccggtttaggtggtgttttaacgaaaacgggtttgggaacactgatcgaaaaaggtaagaaaaagatcagtatcaatgggaccgaataccttttagaattgccactgactgccgatgttgcactcattaagggcagcatcgtggatgaagcaggcaatacattctataaaggcactaccaaaaactttaacccctatatggctatggccgcaaaaaccgtgattgttgaagccgagaatctcgtgtcttgtgaaaaactggaaaaagaaaaggccatgaccccgggtgtcttgattaattacatcgtaaaagagccggcacatcaccatcatcaccattaataa
BBa_K1669002_sequence
1
atgaatagcaaaattattcgtttcgaaaacctgcgtagctttttcaaggatgggatgaccattatgatcggggggttcttaaattgtgggacaccaaccaaactgattgattttctcgtgaacctgaatattaaaaacttgacaattatcagcaacgacacctgctaccctaacaccggtatcggcaaactgatctcaaataaccaggtgaaaaaattgattgcctcctacatcggctccaatccggatactggcaaaaaactgttcaataacgaacttgaggttgaactgagcccccaaggtacccttgtggaacgtattcgtgcggggggttccggtttaggtggtgttttaacgaaaacgggtttgggaacactgatcgaaaaaggtaagaaaaagatcagtatcaatgggaccgaataccttttagaattgccactgactgccgatgttgcactcattaagggcagcatcgtggatgaagcaggcaatacattctataaaggcactaccaaaaactttaacccctatatggctatggccgcaaaaaccgtgattgttgaagccgagaatctcgtgtcttgtgaaaaactggaaaaagaaaaggccatgaccccgggtgtcttgattaattacatcgtaaaagagccggcacatcaccatcatcaccattaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_K823017_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z