BBa_K1669003 1 BBa_K1669003 CtfB + His-tag 2015-09-12T11:00:00Z 2015-09-13T02:03:36Z Sequence retrieved from GenBank and codon optimized for expression in E. coli This part includes the CtfB gene from Clostridium acetobutylicum (NCBI: CAP0163) with a C-terminal His-tag, which has been codon optimized for expression in E. coli. This is one of the two polipeptide chains forming the CtfAB (CoA-transferase) enzyme, that is in Clostridium acetobutylicum responsible for conversion of butyrate to butyril-CoA. The sequence contains a C-terminal His-tag for purification of the protein using the mmobilized-metal affinity chromatography and detected with the anti-His antibodyes. false false _2087_ 26210 26210 9 false C-terminal His-tag. false Nina Jerala annotation2453290 1 ATG range2453290 1 1 3 annotation2453291 1 CtfB range2453291 1 1 673 annotation2453292 1 His-tag range2453292 1 674 682 annotation2453293 1 double stop codon range2453293 1 683 687 BBa_K1669003_sequence 1 atgattaatgataaaaatcttgccaaggaaatcatcgcgaaacgcgtggcgcgggaacttaaaaatggccagctggtgaacctgggcgttggcctgccgaccatggtagcggattatatcccgaaaaattttaaaatcacctttcagagcgagaacgggattgtgggtatgggcgcatcaccaaaaattaatgaagccgataaagatgttgtgaatgcgggtggcgattacaccacggtgttgcctgatggtacgttcttcgatagcagcgtgtctttttcgcttatccgcggtggccacgtagatgtaacggtgctcggtgcgttacaggtggacgaaaagggtaatatcgcgaactggatcgtccctggtaagatgctgtccggaatgggtggtgcaatggatcttgtcaacggcgctaagaaagttattattgccatgcgtcacacgaacaagggacagccgaaaatccttaaaaagtgcaccttgccacttacggcgaaatcacaagcaaatctcattgtgactgaactgggcgtgatcgaagtgattaatgacggcctgttgctgacagaaatcaacaaaaacacgaccatcgatgaaattcgtagtctgactgccgccgatctgcttattagcaacgaactgcgcccgatggcggtgcatcaccatcatcaccattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z