LOCUS BBa_K1669007 842 bp DNA linear 25-Nov-2024 DEFINITION CtfA with strong promotor, strong RBS and double terminator ACCESSION BBa_K1669007 VERSION BBa_K1669007.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23104 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 1..55 /label=BBa_K608002 misc_feature 62..64 /label=ATG misc_feature 730..734 /label=double stop codon misc_feature 711..729 /label=His-tag CDS 62..710 /label=CtfA CDS 62..739 /label=BBa_K1669001 stem_loop 755..774 /label=T7 TE misc_feature 781..781 /label=stop polyA_site 775..788 /label=polya misc_feature 748..788 /label=BBa_B0012 terminator 748..788 /label=BBa_B0012 stem_loop 809..831 /label=stem_loop misc_feature 797..842 /label=BBa_B0011 terminator 797..842 /label=BBa_B0011 terminator 748..842 /label=BBa_B0014 terminator 748..842 /label=BBa_K823017 ORIGIN 1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta 61 gatgaatagc aaaattattc gtttcgaaaa cctgcgtagc tttttcaagg atgggatgac 121 cattatgatc ggggggttct taaattgtgg gacaccaacc aaactgattg attttctcgt 181 gaacctgaat attaaaaact tgacaattat cagcaacgac acctgctacc ctaacaccgg 241 tatcggcaaa ctgatctcaa ataaccaggt gaaaaaattg attgcctcct acatcggctc 301 caatccggat actggcaaaa aactgttcaa taacgaactt gaggttgaac tgagccccca 361 aggtaccctt gtggaacgta ttcgtgcggg gggttccggt ttaggtggtg ttttaacgaa 421 aacgggtttg ggaacactga tcgaaaaagg taagaaaaag atcagtatca atgggaccga 481 atacctttta gaattgccac tgactgccga tgttgcactc attaagggca gcatcgtgga 541 tgaagcaggc aatacattct ataaaggcac taccaaaaac tttaacccct atatggctat 601 ggccgcaaaa accgtgattg ttgaagccga gaatctcgtg tcttgtgaaa aactggaaaa 661 agaaaaggcc atgaccccgg gtgtcttgat taattacatc gtaaaagagc cggcacatca 721 ccatcatcac cattaataat actagagtca cactggctca ccttcgggtg ggcctttctg 781 cgtttatata ctagagagag aatataaaaa gccagattat taatccggct tttttattat 841 tt //