LOCUS       BBa_K1669008             851 bp    DNA     linear       04-May-2025
DEFINITION  CtfB with strong promotor, strong RBS and double terminator
ACCESSION   BBa_K1669008
VERSION     BBa_K1669008.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23104
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_K608002
     misc_feature    62..64
                     /label=ATG
     CDS             62..734
                     /label=CtfB
     misc_feature    735..743
                     /label=His-tag
     misc_feature    744..748
                     /label=double stop codon
     CDS             62..748
                     /label=BBa_K1669003
     stem_loop       764..783
                     /label=T7 TE
     misc_feature    790..790
                     /label=stop
     polyA_site      784..797
                     /label=polya
     misc_feature    757..797
                     /label=BBa_B0012
     terminator      757..797
                     /label=BBa_B0012
     stem_loop       818..840
                     /label=stem_loop
     misc_feature    806..851
                     /label=BBa_B0011
     terminator      806..851
                     /label=BBa_B0011
     terminator      757..851
                     /label=BBa_B0014
     terminator      757..851
                     /label=BBa_K823017
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta
       61 gatgattaat gataaaaatc ttgccaagga aatcatcgcg aaacgcgtgg cgcgggaact
      121 taaaaatggc cagctggtga acctgggcgt tggcctgccg accatggtag cggattatat
      181 cccgaaaaat tttaaaatca cctttcagag cgagaacggg attgtgggta tgggcgcatc
      241 accaaaaatt aatgaagccg ataaagatgt tgtgaatgcg ggtggcgatt acaccacggt
      301 gttgcctgat ggtacgttct tcgatagcag cgtgtctttt tcgcttatcc gcggtggcca
      361 cgtagatgta acggtgctcg gtgcgttaca ggtggacgaa aagggtaata tcgcgaactg
      421 gatcgtccct ggtaagatgc tgtccggaat gggtggtgca atggatcttg tcaacggcgc
      481 taagaaagtt attattgcca tgcgtcacac gaacaaggga cagccgaaaa tccttaaaaa
      541 gtgcaccttg ccacttacgg cgaaatcaca agcaaatctc attgtgactg aactgggcgt
      601 gatcgaagtg attaatgacg gcctgttgct gacagaaatc aacaaaaaca cgaccatcga
      661 tgaaattcgt agtctgactg ccgccgatct gcttattagc aacgaactgc gcccgatggc
      721 ggtgcatcac catcatcacc attaataata ctagagtcac actggctcac cttcgggtgg
      781 gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt aatccggctt
      841 ttttattatt t
//