LOCUS       BBa_K1669009            1358 bp    DNA     linear       25-Nov-2024
DEFINITION  BdhB with strong promotor, strong RBS and double terminator
ACCESSION   BBa_K1669009
VERSION     BBa_K1669009.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23104
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_K608002
     misc_feature    62..64
                     /label=ATG
     CDS             62..1231
                     /label=BdhB
     misc_feature    1232..1250
                     /label=His-tag
     misc_feature    1251..1255
                     /label=double stop codon
     CDS             62..1255
                     /label=BBa_K1669004
     stem_loop       1271..1290
                     /label=T7 TE
     misc_feature    1297..1297
                     /label=stop
     polyA_site      1291..1304
                     /label=polya
     misc_feature    1264..1304
                     /label=BBa_B0012
     terminator      1264..1304
                     /label=BBa_B0012
     stem_loop       1325..1347
                     /label=stem_loop
     misc_feature    1313..1358
                     /label=BBa_B0011
     terminator      1313..1358
                     /label=BBa_B0011
     terminator      1264..1358
                     /label=BBa_B0014
     terminator      1264..1358
                     /label=BBa_K823017
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta
       61 gatggtggat ttcgaatatt cgattcccac acgcatcttt ttcggcaagg acaaaattaa
      121 tgtgctcggg cgggagttga aaaaatacgg ctccaaggtc ctgatcgtgt acggcggtgg
      181 ctcgatcaag cgcaatggca tttatgataa ggctgtaagc attctggaaa aaaactctat
      241 caagttctat gaactggcgg gtgtcgagcc aaacccgcgt gttactaccg tcgaaaaagg
      301 tgtaaaaatc tgccgtgaaa atggcgtaga agtggtgctt gcgattggtg gcggctctgc
      361 gattgattgc gcaaaagtga ttgcagcagc gtgtgaatat gatggtaatc cgtgggacat
      421 tgtgctggat ggtagcaaga ttaaacgtgt actgcctatc gcgtcaattc tgaccatcgc
      481 ggccacaggc tcggagatgg atacatgggc agtgattaat aacatggaca ccaatgaaaa
      541 gttaattgcc gcacatccag acatggcccc gaaattttcg attctggacc cgacctatac
      601 ctataccgtg cccaccaacc agaccgccgc cggaaccgct gatattatgt ctcatatttt
      661 cgaagtatat ttttctaata cgaaaaccgc ctatttacaa gaccgtatgg ccgaagcact
      721 gctgcgcacg tgcatcaaat atggcggaat cgcgctggag aaacctgatg actacgaggc
      781 tcgtgccaac cttatgtggg ctagttcact cgccattaat ggtttgctca cgtacggtaa
      841 ggacaccaat tggtccgtac acctgatgga gcatgaatta agtgcatact acgacattac
      901 gcatggcgtg ggtctggcaa ttctcacacc taactggatg gaatacattc tgaacaacga
      961 cacggtgtac aagtttgtgg aatatggggt taatgtttgg ggtattgata aagagaaaaa
     1021 tcactacgat attgcacacc aggcgattca gaagacccgc gattattttg tgaacgtcct
     1081 cggtctcccc agccgtttgc gcgatgtcgg cattgaagaa gaaaagctgg acatcatggc
     1141 taaagaaagc gtaaaactga ccggcggtac tattggtaac ctgcgtcccg ttaatgcgag
     1201 cgaagtgctg caaattttca agaaaagtgt acatcaccat catcaccatt aataatacta
     1261 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata
     1321 taaaaagcca gattattaat ccggcttttt tattattt
//