BBa_K1669009
type: ComponentDefinition
persistent identity: BBa K1669009
display id: BBa_K1669009
version: 1
was generated by: iGEM to SBOL conversion
description: BdhB with strong promotor, strong RBS and double terminator
created: 2015-09-12T11:00:00Z
modified: 2015-09-13T02:20:27Z
mutable provenance: /
owned by: james
owned by: myers
mutable description: Bdhb fused with strong promotor, strong RBS and double terminator
discontinued: false
dominant: false
group u list: _2087_
m user id: 26210
owner id: 26210
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: /
star: false
creator: Nina Jerala
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1669004
component: BBa_K608002
component: BBa_K823017
sequence annotation: BBa_K608002
sequence annotation: BBa_K1669004
sequence annotation: BBa_K823017
sequence: https://synbiohub.org/public/igem/BBa_K1669009_sequence/1

BBa_K1669004
type: Component
persistent identity: component2453394
display id: component2453394
version: 1
top level: BBa_K1669009
definition: BBa_K1669004
access: public

BBa_K608002
type: Component
persistent identity: component2453389
display id: component2453389
version: 1
top level: BBa_K1669009
definition: BBa_K608002
access: public

BBa_K823017
type: Component
persistent identity: component2453402
display id: component2453402
version: 1
top level: BBa_K1669009
definition: BBa_K823017
access: public

BBa_K608002
type: SequenceAnnotation
persistent identity: annotation2453389
display id: annotation2453389
version: 1
top level: BBa_K1669009
location: https://synbiohub.org/public/igem/BBa_K1669009/annotation2453389/range2453389/1
component: BBa_K608002

https://synbiohub.org/public/igem/BBa_K1669009/annotation2453389/range2453389/1
type: Range
persistent identity: range2453389
display id: range2453389
version: 1
top level: BBa_K1669009
direction: direction/reverse
start: 1
end: 55
orientation: inline

BBa_K1669004
type: SequenceAnnotation
persistent identity: annotation2453394
display id: annotation2453394
version: 1
top level: BBa_K1669009
location: https://synbiohub.org/public/igem/BBa_K1669009/annotation2453394/range2453394/1
component: BBa_K1669004

https://synbiohub.org/public/igem/BBa_K1669009/annotation2453394/range2453394/1
type: Range
persistent identity: range2453394
display id: range2453394
version: 1
top level: BBa_K1669009
direction: direction/reverse
start: 62
end: 1255
orientation: inline

BBa_K823017
type: SequenceAnnotation
persistent identity: annotation2453402
display id: annotation2453402
version: 1
top level: BBa_K1669009
location: https://synbiohub.org/public/igem/BBa_K1669009/annotation2453402/range2453402/1
component: BBa_K823017

https://synbiohub.org/public/igem/BBa_K1669009/annotation2453402/range2453402/1
type: Range
persistent identity: range2453402
display id: range2453402
version: 1
top level: BBa_K1669009
direction: direction/reverse
start: 1264
end: 1358
orientation: inline

BBa_K1669004
type: ComponentDefinition
persistent identity: BBa K1669004
display id: BBa_K1669004
version: 1
was generated by: iGEM to SBOL conversion
description: BdhB + His-tag
created: 2015-09-12T11:00:00Z
modified: 2015-09-13T02:00:27Z
mutable provenance: Sequence retrieved from GenBank and codon optimized for expression in E. coli
owned by: james
owned by: myers
mutable description: This part includes the BdhB gene from Clostridium acetobutylicum (NCBI: CAP0163) with a C-terminal His-tag, which has been codon optimized for expression in E. coli. This is the gene coding the butanol dehydrogenase enzyme, that is in Clostridium acetobutylicum responsible for conversion of butyryl-CoA to butanol. The sequence contains a C-terminal His-tag for purification of the protein using the immobilized-metal affinity chromatography and detected with the anti-His antibodyes.
discontinued: false
dominant: false
group u list: _2087_
m user id: 26210
owner id: 26210
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: C-terminal His-tag.
star: false
creator: Nina Jerala
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BdhB
sequence annotation: ATG
sequence annotation: His-tag
sequence annotation: double stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1669004_sequence/1

BdhB
type: SequenceAnnotation
persistent identity: annotation2453295
display id: annotation2453295
version: 1
top level: BBa_K1669004
location: https://synbiohub.org/public/igem/BBa_K1669004/annotation2453295/range2453295/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1669004/annotation2453295/range2453295/1
type: Range
persistent identity: range2453295
display id: range2453295
version: 1
top level: BBa_K1669004
direction: direction/forward
start: 1
end: 1170
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2453294
display id: annotation2453294
version: 1
top level: BBa_K1669004
location: https://synbiohub.org/public/igem/BBa_K1669004/annotation2453294/range2453294/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1669004/annotation2453294/range2453294/1
type: Range
persistent identity: range2453294
display id: range2453294
version: 1
top level: BBa_K1669004
direction: direction/forward
start: 1
end: 3
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2453296
display id: annotation2453296
version: 1
top level: BBa_K1669004
location: https://synbiohub.org/public/igem/BBa_K1669004/annotation2453296/range2453296/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1669004/annotation2453296/range2453296/1
type: Range
persistent identity: range2453296
display id: range2453296
version: 1
top level: BBa_K1669004
direction: direction/forward
start: 1171
end: 1189
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2453297
display id: annotation2453297
version: 1
top level: BBa_K1669004
location: https://synbiohub.org/public/igem/BBa_K1669004/annotation2453297/range2453297/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1669004/annotation2453297/range2453297/1
type: Range
persistent identity: range2453297
display id: range2453297
version: 1
top level: BBa_K1669004
direction: direction/forward
start: 1190
end: 1194
orientation: inline

BBa_K608002
type: ComponentDefinition
persistent identity: BBa K608002
display id: BBa_K608002
version: 1
was generated by: iGEM to SBOL conversion
description: strong Promoter and strong RBS
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:52Z
mutable provenance: assembly from iGEM parts
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: you can insert your part behind this part so it will be immediatly expressed in e.coli.
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 9115
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: cloning via 3a-assembly
star: false
creator: Julia M??ller
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_J23104
sequence annotation: BBa_J23104
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K608002_sequence/1

BBa_B0034
type: Component
persistent identity: component2128648
display id: component2128648
version: 1
top level: BBa_K608002
definition: BBa_B0034
access: public

BBa_J23104
type: Component
persistent identity: component2128646
display id: component2128646
version: 1
top level: BBa_K608002
definition: BBa_J23104
access: public

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2128646
display id: annotation2128646
version: 1
top level: BBa_K608002
location: https://synbiohub.org/public/igem/BBa_K608002/annotation2128646/range2128646/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K608002/annotation2128646/range2128646/1
type: Range
persistent identity: range2128646
display id: range2128646
version: 1
top level: BBa_K608002
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2128648
display id: annotation2128648
version: 1
top level: BBa_K608002
location: https://synbiohub.org/public/igem/BBa_K608002/annotation2128648/range2128648/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K608002/annotation2128648/range2128648/1
type: Range
persistent identity: range2128648
display id: range2128648
version: 1
top level: BBa_K608002
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K823017
type: ComponentDefinition
persistent identity: BBa K823017
display id: BBa_K823017
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2012-09-10T11:00:00Z
modified: 2015-05-08T01:13:30Z
mutable provenance: Part:BBa_B0014
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is a copy of the Part:B0014. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This is a copy of the Part:B0014. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3.
star: false
creator: Jara Radeck
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0014
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K823017_sequence/1

BBa_B0014
type: Component
persistent identity: component2182657
display id: component2182657
version: 1
top level: BBa_K823017
definition: BBa_B0014
access: public

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2182657
display id: annotation2182657
version: 1
top level: BBa_K823017
location: https://synbiohub.org/public/igem/BBa_K823017/annotation2182657/range2182657/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K823017/annotation2182657/range2182657/1
type: Range
persistent identity: range2182657
display id: range2182657
version: 1
top level: BBa_K823017
direction: direction/reverse
start: 1
end: 95
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

BBa_J23104
type: ComponentDefinition
persistent identity: BBa J23104
display id: BBa_J23104
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
mutable description: replace later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23104_sequence/1

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K1669009_sequence/1
type: Sequence
persistent identity: BBa K1669009 sequence
display id: BBa_K1669009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagatggtggatttcgaatattcgattcccacacgcatctttttcggcaaggacaaaattaatgtgctcgggcgggagttgaaaaaatacggctccaaggtcctgatcgtgtacggcggtggctcgatcaagcgcaatggcatttatgataaggctgtaagcattctggaaaaaaactctatcaagttctatgaactggcgggtgtcgagccaaacccgcgtgttactaccgtcgaaaaaggtgtaaaaatctgccgtgaaaatggcgtagaagtggtgcttgcgattggtggcggctctgcgattgattgcgcaaaagtgattgcagcagcgtgtgaatatgatggtaatccgtgggacattgtgctggatggtagcaagattaaacgtgtactgcctatcgcgtcaattctgaccatcgcggccacaggctcggagatggatacatgggcagtgattaataacatggacaccaatgaaaagttaattgccgcacatccagacatggccccgaaattttcgattctggacccgacctatacctataccgtgcccaccaaccagaccgccgccggaaccgctgatattatgtctcatattttcgaagtatatttttctaatacgaaaaccgcctatttacaagaccgtatggccgaagcactgctgcgcacgtgcatcaaatatggcggaatcgcgctggagaaacctgatgactacgaggctcgtgccaaccttatgtgggctagttcactcgccattaatggtttgctcacgtacggtaaggacaccaattggtccgtacacctgatggagcatgaattaagtgcatactacgacattacgcatggcgtgggtctggcaattctcacacctaactggatggaatacattctgaacaacgacacggtgtacaagtttgtggaatatggggttaatgtttggggtattgataaagagaaaaatcactacgatattgcacaccaggcgattcagaagacccgcgattattttgtgaacgtcctcggtctccccagccgtttgcgcgatgtcggcattgaagaagaaaagctggacatcatggctaaagaaagcgtaaaactgaccggcggtactattggtaacctgcgtcccgttaatgcgagcgaagtgctgcaaattttcaagaaaagtgtacatcaccatcatcaccattaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1669004_sequence/1
type: Sequence
persistent identity: BBa K1669004 sequence
display id: BBa_K1669004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtggatttcgaatattcgattcccacacgcatctttttcggcaaggacaaaattaatgtgctcgggcgggagttgaaaaaatacggctccaaggtcctgatcgtgtacggcggtggctcgatcaagcgcaatggcatttatgataaggctgtaagcattctggaaaaaaactctatcaagttctatgaactggcgggtgtcgagccaaacccgcgtgttactaccgtcgaaaaaggtgtaaaaatctgccgtgaaaatggcgtagaagtggtgcttgcgattggtggcggctctgcgattgattgcgcaaaagtgattgcagcagcgtgtgaatatgatggtaatccgtgggacattgtgctggatggtagcaagattaaacgtgtactgcctatcgcgtcaattctgaccatcgcggccacaggctcggagatggatacatgggcagtgattaataacatggacaccaatgaaaagttaattgccgcacatccagacatggccccgaaattttcgattctggacccgacctatacctataccgtgcccaccaaccagaccgccgccggaaccgctgatattatgtctcatattttcgaagtatatttttctaatacgaaaaccgcctatttacaagaccgtatggccgaagcactgctgcgcacgtgcatcaaatatggcggaatcgcgctggagaaacctgatgactacgaggctcgtgccaaccttatgtgggctagttcactcgccattaatggtttgctcacgtacggtaaggacaccaattggtccgtacacctgatggagcatgaattaagtgcatactacgacattacgcatggcgtgggtctggcaattctcacacctaactggatggaatacattctgaacaacgacacggtgtacaagtttgtggaatatggggttaatgtttggggtattgataaagagaaaaatcactacgatattgcacaccaggcgattcagaagacccgcgattattttgtgaacgtcctcggtctccccagccgtttgcgcgatgtcggcattgaagaagaaaagctggacatcatggctaaagaaagcgtaaaactgaccggcggtactattggtaacctgcgtcccgttaatgcgagcgaagtgctgcaaattttcaagaaaagtgtacatcaccatcatcaccattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K608002_sequence/1
type: Sequence
persistent identity: BBa K608002 sequence
display id: BBa_K608002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K823017_sequence/1
type: Sequence
persistent identity: BBa K823017 sequence
display id: BBa_K823017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23104_sequence/1
type: Sequence
persistent identity: BBa J23104 sequence
display id: BBa_J23104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtattgtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z