BBa_K1670001 1 BBa_K1670001 3OC6-HSL receiver device 2015-08-23T11:00:00Z 2015-09-03T02:45:04Z Genome sequence of <i> Erwinia stewartii </i> BBa_K1670001 contains the EsaR/I regulated esaRC promoter, an engineered variant of the native esaR-promoter from <i> Erwinia stewartii </i> that contains two EsaR-boxes at the -10 region and between the -10 and -35 region[3] that allow a tighter control of the expression of the genes under the control of P_esaRC. The part already contains a codon optimized CFP based on BBa_E0020 with a synthetic ribosome-binding site as a fluorescent reporter. eCFP can be replaced using the NdeI restriction site in the start codon as well as the biobrick suffix. false false _2088_ 24905 24905 9 false two EsaR-boxes for tight controll of eCFP expression false Christoph Schilling annotation2436900 1 esa-box range2436900 1 28 47 annotation2443064 1 STOP range2443064 1 838 843 annotation2443062 1 eCFP range2443062 1 121 837 annotation2436899 1 esaRC range2436899 1 1 96 annotation2436901 1 esa-box range2436901 1 49 68 annotation2443058 1 RBS range2443058 1 97 120 BBa_K1670001_sequence 1 ttgtaacctctgaatgattcattgtaagcctgtactatagtgcaggttgcctgtactatagtgcaggttaagtccacgttaagtaaaagaagcagcgccaaaatttaagtaggtccgcatatggtctctaagggggaagagctgttcacaggcgtcgttcccattttggttgagttagatggtgatgtgaacgggcacaaattctccgtgagcggtgagggcgaaggcgacgcgacctacggcaaactcaccttgaaatttatttgtactaccggaaaactgccagtgccctggccgacactggtcaccacactgacttggggcgttcaatgttttagccgttacccggatcacatgaaacaacacgatttcttcaaatccgcaatgccggaaggctatgtgcaggagcgtacgatctttttcaaggatgatggtaactataaaactcgtgcggaagttaaattcgaaggcgataccttagtgaaccgcattgaactgaaaggcattgatttcaaagaagatggtaacattttaggccacaagcatgaatataactatattagtcacaacgtgtatatcacggcggacaagcaaaaaaatggtattaaggccaactttaaaattcggcacaacatcgaagatggctcagtgcaattagccgaccactatcagcagaatacccctattggtgacggcccggtgctgttgccggataaccactacttatctacccaaagcgctctctccaaggacccgaacgagaaacgtgatcatacggtgctgcttgagttcgttaccgcagcaggcatcaccctgggaatggatgaactgtacaaatgataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z