BBa_K1670002 1 BBa_K1670002 cepR activator 2015-08-24T11:00:00Z 2015-08-25T02:41:25Z Sequencing of Burkholderia cepacia GenBank: AJ422183.1 BBa_K1670002 (CepR) is the regulatory counterpart of the CepR/I quorum sensing system of <i>Bulkholderia cenocepacia</i>. The protein forms inclusion bodies in the absence of C8-HSL and needs to bind C8-HSL to stay in a soluble form [1] When a concentration of 80-100 nM C8-HSL is reached, the homoserine lactone binds CepR and induces a dimerization of the regulatory protein and allows binding to its corresponding DNA-binding site in an imperfect dyad manner[1] and works as an activator of the corresponding promoter. false false _2088_ 24905 24905 9 false The gene is codon optimized for E.coli BL21 and contains a synthetic ribosome binding site. false Christoph Schilling annotation2437877 1 cepR range2437877 1 36 752 annotation2437876 1 RBS range2437876 1 1 35 annotation2437878 1 STOP range2437878 1 753 758 BBa_K1670002_sequence 1 cacgttaaatacacacgaagctaaggaggtcaaaaatggagctgcgttggcaggatgcgtaccaacaattcagtgcggctgaagacgaacaacaattgttccagcggatcgcagcgtatagtaaacgtctcggattcgaatattgttgctacggcattcgtgtcccgctgccagtgtcgaaaccgtctgtggcgatcttcgatacatatccggacggctggatggcccactatcaagcacataactacatcgaaattgacagcacggtacgtgatggcgcgctgtctacgaatatgattgtgtggccggatgtggaccagattgagccttgcccgctctggcaggatgcgcgtgatttcggtctgagcgttggggttgcacaatcgtcctgggccgcgcgcggagccttcggcctgctgagtatcgcgcgccatgctgatcggctgaccccggctgaaattaacatgttgacgctccaaacgaattggttagcgaacttgagtcattccctgatgtcccggttcatggtgccgaaattaagtcctgctgccggggtcacgctcaccgcacgggaacgtgaggttctctgctggacggcagaaggcaagacagcgtgcgaaattggccaaattctgagcatttctgaacgcacggtgaattttcacgtgaataacattttggaaaaattgggcgcgactaataaggtgcaagctgtcgtcaaagcaatttccgccgggctgatcgaggcgccatgataatccataat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z