BBa_K1670008
1
BBa_K1670008
Tyrosinase
2015-09-15T11:00:00Z
2015-09-16T04:22:52Z
Marinomonas mediterranea
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BBa_K1670008 codes for tyrosinase from <i>Marinomonas mediterranea</i>, a gram negative bacterium, which should facilitate expression in <i>E. coli</i>. Tyrosinases are native to several eukaryotic and prokaryotic species including fungi and bacteria. In its endogenous form, the tyrosinase is a part of the melanin biosynthesis pathway, converting L-Tyrosine to L-DOPA and further to dopaquinone. Melanins, dark pigments, protect the bacterial cells and spores against UV radiation, bind cytotoxic heavy metals, confer protection against oxidants, heat, enzymatic hydrolysis, antimicrobial compounds and phagocytosis (1).
Tyrosinase from <i>M. mediterranea</i> requires the chaperone
<a href="http://parts.igem.org/Part:BBa_K1670009">BBa_K1670009</a>
which is responsible for the delivery of copper to the tyrosinase which enhances production of L-DOPA.(2)
<p>References
1. Claus, Harald ; Decker, Heinz. (2006). Bacterial tyrosinases. Systematic and Applied Microbiology. 29 (1), p3-14.
2. Lopez-Serrano, D., Solano, F. and Sanchez-Amat, A. (2007). Involvement of a novel copper chaperone in tyrosinase activity and melanin synthesis in Marinomonas mediterranea. Microbiology, 153(7), pp.2241-2249.
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false
false
_2088_
24842
24842
9
false
Sequence was checked for internal BioBrick restriction sites and codon optimised for E. coli BL21.
false
Magdalena Hannah Kurteu
BBa_K1670008_sequence
1
atggcaatccgggtccgcaagaacgttaatgaactcaccgacgacacgctgctgtggtatagcaaagcggtggagtcgatgaagcagaaagacatcaccgacccgagcagttggtggtaccagggtgcgattcacggctacggcctggataaacgcccaaacttggcgaacaatgagagctggagcgaatccagcgtctgggaacaggccgagggctttcccccgtcagaaggtctggtcaactctcagttttggcagcagtgccagcacggtacctggttttttctgccgtggcatcgcatgtatcttcagttttttgaggccatcgttgctaaaactgttgtcgaactcggcggccccaaagattggacattgccgtattggaactattgtgacgccaacaatccagcgctgaatccgactgagcagttacaggccctgaaattacccagcgaatttggtacaaatacgccgaaccctgactttccgggcctgtggatgaaagaacgcgcacagtatcagctgtcttcgcaggcggatgcatcgtgctcggtagcaatgaaattacaaaatttcactgcatcctcgccggcaacgtcattcggtggcgtccagaccggtttttcccatgatagcggtacgttcggagcggtggaaaataatccgcataacctcgttcatgtggacatcggcggcgcgatgggtgatcccaatacagcggccctggaccccattttctggctgcatcacgcaaacatcgatcgtctgtggcaatgttggatcgatcagggtcgcgagaatactaatgatattacgtggctgaaccaggtctttgacttccacaatgcggattcactgccagacacgctgtcagtgaaagacgtgctctcgactgaagctctgggttttacatacagcgattcttattcttctagtgctgcaccaaccgatagcaaagtgttcgccctggcgagtgcgggcgggtcggggatgttcgacaccatcgcggcaaccacaaaaccgtttctgctgggcagccaatcgaccagcgctcagctggaatttctgccggaaaaacagcgcgccgctcaagtgcccgtattaggcgcgagcaactcgcagaccccaaaccaggttatcatcgttcttgacaacgtcaccggtagcggtgttgtggctccagtgtcagtttacgtaaaggcctccgccaacagtgaacgcgtcctcgtggggaaaattggcctgtttggcttgacgcaatccagcacgccgtcatcgacttcatgtctggagcagggaattagcatcgaactggatgtgacagacgcgctgcaacaactgcgtagtcagacaaattggaacctggagaacctgcaaattgagttggaaccgggccgtgaactgggtaacgcctcggtaaccgtcgggcgcgtgtcaattaaggctgaagtggttggttcaggacatcaccaccaccatcactag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z