BBa_K1670008 1 BBa_K1670008 Tyrosinase 2015-09-15T11:00:00Z 2015-09-16T04:22:52Z Marinomonas mediterranea <html> <p> BBa_K1670008 codes for tyrosinase from <i>Marinomonas mediterranea</i>, a gram negative bacterium, which should facilitate expression in <i>E. coli</i>. Tyrosinases are native to several eukaryotic and prokaryotic species including fungi and bacteria. In its endogenous form, the tyrosinase is a part of the melanin biosynthesis pathway, converting L-Tyrosine to L-DOPA and further to dopaquinone. Melanins, dark pigments, protect the bacterial cells and spores against UV radiation, bind cytotoxic heavy metals, confer protection against oxidants, heat, enzymatic hydrolysis, antimicrobial compounds and phagocytosis (1). Tyrosinase from <i>M. mediterranea</i> requires the chaperone <a href="http://parts.igem.org/Part:BBa_K1670009">BBa_K1670009</a> which is responsible for the delivery of copper to the tyrosinase which enhances production of L-DOPA.(2) <p>References 1. Claus, Harald ; Decker, Heinz. (2006). Bacterial tyrosinases. Systematic and Applied Microbiology. 29 (1), p3-14. 2. Lopez-Serrano, D., Solano, F. and Sanchez-Amat, A. (2007). Involvement of a novel copper chaperone in tyrosinase activity and melanin synthesis in Marinomonas mediterranea. Microbiology, 153(7), pp.2241-2249. </p> </html> false false _2088_ 24842 24842 9 false Sequence was checked for internal BioBrick restriction sites and codon optimised for E. coli BL21. false Magdalena Hannah Kurteu BBa_K1670008_sequence 1 atggcaatccgggtccgcaagaacgttaatgaactcaccgacgacacgctgctgtggtatagcaaagcggtggagtcgatgaagcagaaagacatcaccgacccgagcagttggtggtaccagggtgcgattcacggctacggcctggataaacgcccaaacttggcgaacaatgagagctggagcgaatccagcgtctgggaacaggccgagggctttcccccgtcagaaggtctggtcaactctcagttttggcagcagtgccagcacggtacctggttttttctgccgtggcatcgcatgtatcttcagttttttgaggccatcgttgctaaaactgttgtcgaactcggcggccccaaagattggacattgccgtattggaactattgtgacgccaacaatccagcgctgaatccgactgagcagttacaggccctgaaattacccagcgaatttggtacaaatacgccgaaccctgactttccgggcctgtggatgaaagaacgcgcacagtatcagctgtcttcgcaggcggatgcatcgtgctcggtagcaatgaaattacaaaatttcactgcatcctcgccggcaacgtcattcggtggcgtccagaccggtttttcccatgatagcggtacgttcggagcggtggaaaataatccgcataacctcgttcatgtggacatcggcggcgcgatgggtgatcccaatacagcggccctggaccccattttctggctgcatcacgcaaacatcgatcgtctgtggcaatgttggatcgatcagggtcgcgagaatactaatgatattacgtggctgaaccaggtctttgacttccacaatgcggattcactgccagacacgctgtcagtgaaagacgtgctctcgactgaagctctgggttttacatacagcgattcttattcttctagtgctgcaccaaccgatagcaaagtgttcgccctggcgagtgcgggcgggtcggggatgttcgacaccatcgcggcaaccacaaaaccgtttctgctgggcagccaatcgaccagcgctcagctggaatttctgccggaaaaacagcgcgccgctcaagtgcccgtattaggcgcgagcaactcgcagaccccaaaccaggttatcatcgttcttgacaacgtcaccggtagcggtgttgtggctccagtgtcagtttacgtaaaggcctccgccaacagtgaacgcgtcctcgtggggaaaattggcctgtttggcttgacgcaatccagcacgccgtcatcgacttcatgtctggagcagggaattagcatcgaactggatgtgacagacgcgctgcaacaactgcgtagtcagacaaattggaacctggagaacctgcaaattgagttggaaccgggccgtgaactgggtaacgcctcggtaaccgtcgggcgcgtgtcaattaaggctgaagtggttggttcaggacatcaccaccaccatcactag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z