LOCUS       BBa_K1673213            1090 bp    DNA     linear       01-Jun-2024
DEFINITION  Mutation Optimized S1 Nuclease with IPTG Promoter
ACCESSION   BBa_K1673213
VERSION     BBa_K1673213.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..1090
                     /label=BBa_K1673203
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc cgcgtctgct
      241 gcccattagc gcagccactt tggcactggc gcaattgacg tatggctggg gtaacctggg
      301 tcacgaaact gtagcatata tcgctcaatc ctttgtggct tcctcgacgg aaagtttttg
      361 tcagaatatt ctgggggatg acagcacctc gtatctggct aacgtcgcca cttgggccga
      421 cacatacaaa tacacagatg ccggggagtt ctcaaagcca tatcatttca ttgacgcgca
      481 ggataatccg ccgcagagtt gtggcgtgga ctatgatcgt gattgtggtt ccgcggggtg
      541 tagtatctcg gctattcaaa attacaccaa tattctgctg gaaagtccga atggctcgga
      601 agctctgaac gctttaaaat tcgtggttca tattattggc gacatccatc aaccgctgca
      661 tgacgagaat ttagaagcag gaggaaatgg tatcgatgtg acttacgatg gtgaaaccac
      721 gaatcttcat catatttggg atacgaatat gcctgaagaa gctgccggtg gctactccct
      781 cagcgttgcg aaaacatatg cggacctgct gaccgaacgc atcaagaccg ggacgtattc
      841 ttccaaaaaa gactcgtgga ctgatgggat cgatatcaag gaccccgtct cgacgagcat
      901 gatttgggcg gcagatgcga atacgtatgt gtgctccacc gtactcgatg atggccttgc
      961 atacattaat agtactgact tgtcgggcga gtactacgac aaatcacagc cggtttttga
     1021 agagctgatt gcgaaggccg ggtatcgttt agctgcgtgg ctcgacctca tcgcttccca
     1081 gccgtcataa
//