LOCUS       BBa_K1673223             902 bp    DNA     linear       22-Dec-2024
DEFINITION  Mutation Optimized S1 Nuclease with T7 Promoter
ACCESSION   BBa_K1673223
VERSION     BBa_K1673223.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     CDS             39..902
                     /label=BBa_K1673203
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat gccgcgtctg ctgcccatta
       61 gcgcagccac tttggcactg gcgcaattga cgtatggctg gggtaacctg ggtcacgaaa
      121 ctgtagcata tatcgctcaa tcctttgtgg cttcctcgac ggaaagtttt tgtcagaata
      181 ttctggggga tgacagcacc tcgtatctgg ctaacgtcgc cacttgggcc gacacataca
      241 aatacacaga tgccggggag ttctcaaagc catatcattt cattgacgcg caggataatc
      301 cgccgcagag ttgtggcgtg gactatgatc gtgattgtgg ttccgcgggg tgtagtatct
      361 cggctattca aaattacacc aatattctgc tggaaagtcc gaatggctcg gaagctctga
      421 acgctttaaa attcgtggtt catattattg gcgacatcca tcaaccgctg catgacgaga
      481 atttagaagc aggaggaaat ggtatcgatg tgacttacga tggtgaaacc acgaatcttc
      541 atcatatttg ggatacgaat atgcctgaag aagctgccgg tggctactcc ctcagcgttg
      601 cgaaaacata tgcggacctg ctgaccgaac gcatcaagac cgggacgtat tcttccaaaa
      661 aagactcgtg gactgatggg atcgatatca aggaccccgt ctcgacgagc atgatttggg
      721 cggcagatgc gaatacgtat gtgtgctcca ccgtactcga tgatggcctt gcatacatta
      781 atagtactga cttgtcgggc gagtactacg acaaatcaca gccggttttt gaagagctga
      841 ttgcgaaggc cgggtatcgt ttagctgcgt ggctcgacct catcgcttcc cagccgtcat
      901 aa
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