LOCUS       BBa_K1674003            1871 bp    DNA     linear       25-Nov-2024
DEFINITION  tsPurple Reporter + 3-alpha-HSD (RBS + Coding + Terminator)
ACCESSION   BBa_K1674003
VERSION     BBa_K1674003.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     CDS             1..43
                     /label=BBa_K1321338
     CDS             50..1018
                     /label=BBa_K1674001
     misc_feature    1031..1034
                     /label=conserved
     RBS             1027..1038
                     /label=BBa_B0034
     CDS             1045..1734
                     /label=tsPurple
     CDS             1045..1734
                     /label=BBa_K1033906
     stem_loop       1754..1797
                     /label=stem_loop
     misc_feature    1743..1822
                     /label=BBa_B0010
     terminator      1743..1822
                     /label=BBa_B0010
     stem_loop       1838..1857
                     /label=T7 TE
     misc_feature    1864..1864
                     /label=stop
     polyA_site      1858..1871
                     /label=polya
     misc_feature    1831..1871
                     /label=BBa_B0012
     terminator      1831..1871
                     /label=BBa_B0012
     terminator      1743..1871
                     /label=BBa_B0015
     misc_feature    1027..1871
                     /label=BBa_K1357008
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggattccat
       61 atctctgcgt gtagcactaa atgatggtaa cttcattcct gtactggggt ttggaaccac
      121 tgtgcctgag aaggttgcta aggatgaagt tatcaaggct actaaaatag ctatagataa
      181 tggattccgc cattttgact ctgcttattt gtacgaagta gaagaggaag tgggccaagc
      241 cattagaagc aagattgaag acggcactgt gaagagagaa gatatattct atacttcaaa
      301 gctttggagc actttccata gaccagagct ggtccgaact tgcttggaaa agacactgaa
      361 aagcactcaa caggactatg tggatcttta tattattcat ttcccaatgg ctttgcagcc
      421 tggagatata tttttcccac gagatgagca tggaaaacta ttgtttgaaa cagtggatat
      481 ctgtgacaca tgggaggcca tggaaaagtg taaggatgca ggattggcca agtctattgg
      541 ggtgtccaac tttaactgta ggcagctgga gaggattctg aataagccag ggctcaaata
      601 caagcctgtg tgcaaccagg tggaatgtca cctttatctc aaccagagca aaatgctgga
      661 ctattgtaag tcaaaagaca tcattctggt ttcctactgc acgctgggaa gttcacgaga
      721 caaaacatgg gtggatcaga aaagtccagt tctcctagat gatccagttc tttgtgccat
      781 agcaaagaag tacaagcaaa ccccagccct agttgccctt cgctaccagc ttcagcgtgg
      841 ggttgtgccc ctgatcagga gtttcaagcc gaagcggatc aaagagccaa cacaggtttt
      901 tgaatttcag ttggcttcag aggacatgaa agccctggat ggcttgaaca gaaatttcag
      961 atacaacaat gcaaaatatt ttgatgacca tcccaatcat ccatttactg atgaatagta
     1021 ctagagaaag aggagaaata ctagatggcg agcttggtta agaaagatat gtgtgttaag
     1081 atgacgatgg aaggtactgt gaacggttat cactttaagt gcgttggcga gggtgaaggc
     1141 aagccgttcg agggcacgca gaacatgcgc attcgtgtca ccgagggcgg tccgctgcct
     1201 tttgcattcg acatcctggc cccgtgctgt atgtacggct ctaagacctt cattaaacac
     1261 gtgagcggta tcccggatta ctttaaagag tcctttccag agggcttcac ttgggaacgt
     1321 acccagattt ttgaggacgg tggtgttctg accgcgcacc aagacaccag cctggaaggt
     1381 aattgcctga tctataaagt gaaggttctg ggtaccaatt tcccggcgaa tggtccggtg
     1441 atgcaaaaga aaaccgcggg ttgggagccg tgcgtcgaga tgctgtatcc gcgtgacggc
     1501 gtcttgtgtg gtcagagctt gatggcgctg aagtgcaccg atggcaatca tctgaccagc
     1561 cacctgcgca cgacgtatcg tagccgtaaa ccgagcaacg ccgttaacat gccggagttc
     1621 cattttggtg accatcgcat cgaaatcctg aaagctgagc agggcaaatt ctacgaacaa
     1681 tacgaatcgg ctgtcgcacg ttacagcgat gtgccggaaa aagcgacgta ataatactag
     1741 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1801 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1861 ctgcgtttat a
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