BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_I719005 1 pT7 T7 Promoter 2007-10-23T11:00:00Z 2015-08-31T04:07:53Z --- Released HQ 2013 Just a T7 Promoter false false _128_ 0 2097 9 In stock true None true Imperial 2007 BBa_K1321338 1 BBa_K1321338 T7 Expression vector 2014-10-07T11:00:00Z 2015-05-08T01:09:53Z asdf asdfasdf false false _1696_ 0 20830 9 In stock false asdf false Chris N Micklem component2402301 1 BBa_B0034 component2402299 1 BBa_I719005 annotation2402301 1 BBa_B0034 range2402301 1 32 43 annotation2402299 1 BBa_I719005 range2402299 1 1 23 BBa_K1033906 1 tsPurple tsPurple, purple chromoprotein 2013-08-24T11:00:00Z 2015-05-08T01:08:49Z Synthetic chromoprotein from DNA 2.0. This chromoprotein naturally exhibits strong color when expressed. false false _1340_ 0 10137 9 In stock false Cloned from TinselPurple vector, DNA 2.0. false Sabri Jamal annotation2332100 1 tsPurple range2332100 1 1 690 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K1674001 1 3alpha-HSD 3alpha-hydroxysteroid dehydrogenase 2015-09-12T11:00:00Z 2015-09-18T02:27:41Z gblock ordered from IDT 3&#945;- HSD false false _2092_ 25765 25765 9 true k false Liron Abrahami BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K1357008 1 BBa_K1357008 tsPurple Reporter (RBS + Coding + Terminator) 2014-10-08T11:00:00Z 2015-05-08T01:10:02Z Coding sequence comes from jellyfish? Reporter to add onto parts to see if they are functional false false _1733_ 0 16772 9 In stock false None false Matthew Tucker component2408700 1 BBa_B0015 component2408691 1 BBa_B0034 component2408693 1 BBa_K1033906 annotation2408691 1 BBa_B0034 range2408691 1 1 12 annotation2408700 1 BBa_B0015 range2408700 1 717 845 annotation2408693 1 BBa_K1033906 range2408693 1 19 708 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1674003 1 BBa_K1674003 tsPurple Reporter + 3-alpha-HSD (RBS + Coding + Terminator) 2015-09-14T11:00:00Z 2015-09-18T02:25:56Z tsPurple Reporter + 3&#945;- HSD tsPurple Reporter + 3&#945;- HSD false false _2092_ 25765 25765 9 false tsPurple Reporter + 3&#945;- HSD false Adi Yannai component2457659 1 BBa_K1674001 component2457658 1 BBa_K1321338 component2457671 1 BBa_K1357008 annotation2457658 1 BBa_K1321338 range2457658 1 1 43 annotation2457671 1 BBa_K1357008 range2457671 1 1027 1871 annotation2457659 1 BBa_K1674001 range2457659 1 50 1018 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_I719005_sequence 1 taatacgactcactatagggaga BBa_B0034_sequence 1 aaagaggagaaa BBa_K1674001_sequence 1 atggattccatatctctgcgtgtagcactaaatgatggtaacttcattcctgtactggggtttggaaccactgtgcctgagaaggttgctaaggatgaagttatcaaggctactaaaatagctatagataatggattccgccattttgactctgcttatttgtacgaagtagaagaggaagtgggccaagccattagaagcaagattgaagacggcactgtgaagagagaagatatattctatacttcaaagctttggagcactttccatagaccagagctggtccgaacttgcttggaaaagacactgaaaagcactcaacaggactatgtggatctttatattattcatttcccaatggctttgcagcctggagatatatttttcccacgagatgagcatggaaaactattgtttgaaacagtggatatctgtgacacatgggaggccatggaaaagtgtaaggatgcaggattggccaagtctattggggtgtccaactttaactgtaggcagctggagaggattctgaataagccagggctcaaatacaagcctgtgtgcaaccaggtggaatgtcacctttatctcaaccagagcaaaatgctggactattgtaagtcaaaagacatcattctggtttcctactgcacgctgggaagttcacgagacaaaacatgggtggatcagaaaagtccagttctcctagatgatccagttctttgtgccatagcaaagaagtacaagcaaaccccagccctagttgcccttcgctaccagcttcagcgtggggttgtgcccctgatcaggagtttcaagccgaagcggatcaaagagccaacacaggtttttgaatttcagttggcttcagaggacatgaaagccctggatggcttgaacagaaatttcagatacaacaatgcaaaatattttgatgaccatcccaatcatccatttactgatgaatag BBa_K1321338_sequence 1 taatacgactcactatagggagatactagagaaagaggagaaa BBa_K1357008_sequence 1 aaagaggagaaatactagatggcgagcttggttaagaaagatatgtgtgttaagatgacgatggaaggtactgtgaacggttatcactttaagtgcgttggcgagggtgaaggcaagccgttcgagggcacgcagaacatgcgcattcgtgtcaccgagggcggtccgctgccttttgcattcgacatcctggccccgtgctgtatgtacggctctaagaccttcattaaacacgtgagcggtatcccggattactttaaagagtcctttccagagggcttcacttgggaacgtacccagatttttgaggacggtggtgttctgaccgcgcaccaagacaccagcctggaaggtaattgcctgatctataaagtgaaggttctgggtaccaatttcccggcgaatggtccggtgatgcaaaagaaaaccgcgggttgggagccgtgcgtcgagatgctgtatccgcgtgacggcgtcttgtgtggtcagagcttgatggcgctgaagtgcaccgatggcaatcatctgaccagccacctgcgcacgacgtatcgtagccgtaaaccgagcaacgccgttaacatgccggagttccattttggtgaccatcgcatcgaaatcctgaaagctgagcagggcaaattctacgaacaatacgaatcggctgtcgcacgttacagcgatgtgccggaaaaagcgacgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1033906_sequence 1 atggcgagcttggttaagaaagatatgtgtgttaagatgacgatggaaggtactgtgaacggttatcactttaagtgcgttggcgagggtgaaggcaagccgttcgagggcacgcagaacatgcgcattcgtgtcaccgagggcggtccgctgccttttgcattcgacatcctggccccgtgctgtatgtacggctctaagaccttcattaaacacgtgagcggtatcccggattactttaaagagtcctttccagagggcttcacttgggaacgtacccagatttttgaggacggtggtgttctgaccgcgcaccaagacaccagcctggaaggtaattgcctgatctataaagtgaaggttctgggtaccaatttcccggcgaatggtccggtgatgcaaaagaaaaccgcgggttgggagccgtgcgtcgagatgctgtatccgcgtgacggcgtcttgtgtggtcagagcttgatggcgctgaagtgcaccgatggcaatcatctgaccagccacctgcgcacgacgtatcgtagccgtaaaccgagcaacgccgttaacatgccggagttccattttggtgaccatcgcatcgaaatcctgaaagctgagcagggcaaattctacgaacaatacgaatcggctgtcgcacgttacagcgatgtgccggaaaaagcgacgtaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1674003_sequence 1 taatacgactcactatagggagatactagagaaagaggagaaatactagatggattccatatctctgcgtgtagcactaaatgatggtaacttcattcctgtactggggtttggaaccactgtgcctgagaaggttgctaaggatgaagttatcaaggctactaaaatagctatagataatggattccgccattttgactctgcttatttgtacgaagtagaagaggaagtgggccaagccattagaagcaagattgaagacggcactgtgaagagagaagatatattctatacttcaaagctttggagcactttccatagaccagagctggtccgaacttgcttggaaaagacactgaaaagcactcaacaggactatgtggatctttatattattcatttcccaatggctttgcagcctggagatatatttttcccacgagatgagcatggaaaactattgtttgaaacagtggatatctgtgacacatgggaggccatggaaaagtgtaaggatgcaggattggccaagtctattggggtgtccaactttaactgtaggcagctggagaggattctgaataagccagggctcaaatacaagcctgtgtgcaaccaggtggaatgtcacctttatctcaaccagagcaaaatgctggactattgtaagtcaaaagacatcattctggtttcctactgcacgctgggaagttcacgagacaaaacatgggtggatcagaaaagtccagttctcctagatgatccagttctttgtgccatagcaaagaagtacaagcaaaccccagccctagttgcccttcgctaccagcttcagcgtggggttgtgcccctgatcaggagtttcaagccgaagcggatcaaagagccaacacaggtttttgaatttcagttggcttcagaggacatgaaagccctggatggcttgaacagaaatttcagatacaacaatgcaaaatattttgatgaccatcccaatcatccatttactgatgaatagtactagagaaagaggagaaatactagatggcgagcttggttaagaaagatatgtgtgttaagatgacgatggaaggtactgtgaacggttatcactttaagtgcgttggcgagggtgaaggcaagccgttcgagggcacgcagaacatgcgcattcgtgtcaccgagggcggtccgctgccttttgcattcgacatcctggccccgtgctgtatgtacggctctaagaccttcattaaacacgtgagcggtatcccggattactttaaagagtcctttccagagggcttcacttgggaacgtacccagatttttgaggacggtggtgttctgaccgcgcaccaagacaccagcctggaaggtaattgcctgatctataaagtgaaggttctgggtaccaatttcccggcgaatggtccggtgatgcaaaagaaaaccgcgggttgggagccgtgcgtcgagatgctgtatccgcgtgacggcgtcttgtgtggtcagagcttgatggcgctgaagtgcaccgatggcaatcatctgaccagccacctgcgcacgacgtatcgtagccgtaaaccgagcaacgccgttaacatgccggagttccattttggtgaccatcgcatcgaaatcctgaaagctgagcagggcaaattctacgaacaatacgaatcggctgtcgcacgttacagcgatgtgccggaaaaagcgacgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z