BBa_K1675005 1 BBa_K1675005 J23119+B0034+NhaB(BBa_K1675001) 2015-09-09T11:00:00Z 2015-09-12T12:25:21Z The sequence of promoter J23119 is found through the standard part BBa_J23119 http://parts.igem.org/Part:BBa_J23119 The sequence of ribosome binding site B0034 is found through the standard part BBa_B0034 http://parts.igem.org/Part:BBa_B0034 The sequence of gene NhaB is found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=1233176&to=1234717&strand=true The constitutive promoter J23119, the ribosome binding site B0034 and the functional gene nhaB were used in this new standard part. This part is available for the bacteria to survive in alkaline environment. false false _2093_ 27740 27740 9 false mutation of CTG to CTA Leu.(site:383bp); mutation of GCT to GCG Ala.(site:461bp); mutation of ACC to ACT Thr.(site:566bp); mutation of GAC to GAT Asp.(site:572bp); mutation of ACC to ACT Thr.(site:677bp); mutation of GTA to GTT Val.(site:692bp); mutation of GCA to GCG Ala.(site:1040bp); mutation of GAG to GAA Glu.(site:1067bp); mutation of GTC to GTA Val.(site:1499bp) false JIAQI XU component2452858 1 BBa_J23119 component2452860 1 BBa_B0034 component2452873 1 BBa_K1675001 annotation2452860 1 BBa_B0034 range2452860 1 44 55 annotation2452858 1 BBa_J23119 range2452858 1 1 35 annotation2452873 1 BBa_K1675001 range2452873 1 62 1603 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_K1675001 1 BBa_K1675001 NhaB; sodium-proton antiporter; membrane protein 2015-09-08T11:00:00Z 2015-09-15T09:57:31Z Escherichia coli str. K-12 substr. Sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=fasta&from=1233176&to=1234717&strand=true The gene, NhaB, is the archetypal sodium-proton antiporter in E. coli. It is crucial when the level of NhaA activity is growth limiting, when NhaA is not sufficiently induced, or when NhaA is not activated. NhaA catalyzes the exchange of 3 proton ion per 2 sodium ion. Therefore, NhaA could make sure E. coli???s survival under alkaline pH, which are in desperate need in our project. false false _2093_ 27740 27740 9 false mutation of CTG to CTA Leu.(site:336bp); mutation of GCT to GCG Ala.(site:414bp); mutation of ACC to ACT Thr.(site:519bp); mutation of GAC to GAT Asp.(site:525bp); mutation of ACC to ACT Thr.(site:630bp); mutation of GTA to GTG Val.(site:645bp); mutation of GCA to GCG Ala.(site:993bp); mutation of GAG to GAA Glu.(site:1020bp); mutation of GTC to GTA Val.(site:1452bp) false JIAQI XU annotation2456967 1 TGA stop codon range2456967 1 1540 1542 annotation2456965 1 NhaB Coding Region range2456965 1 1 1542 annotation2456966 1 ATG start codon range2456966 1 1 3 BBa_K1675001_sequence 1 atggagatctcctggggccgcgcgctatggcgcaattttttgggccagtcccccgactggtacaaactcgccctcatcattttcttaatcgtaaacccgttaattttcctcatcagccctttcgtcgcgggctggttgctggtagcggaatttattttcactctggcgatggccctgaaatgctacccgctgctccccggtggtctgttggctatcgaagcggtattcatcggcatgaccagcgcggaacacgtccgtgaagaggtggcggcaaatcttgaagtcttgctgttactgatgtttatggtggcgggtatctattttatgaaacagctattgctgttcatatttacccgtttgctgttaagcattcgctccaaaatgctgctgtcgctctctttttgcgtggcggcggcgttcctctccgcgttcctcgatgccttaaccgtcgtggcggtggtgatcagcgttgcagtcggtttttatggtatttatcaccgcgtagcctcttcccgtactgaagataccgacctgcaagacgatagtcatatcgacaagcattacaaagtggttctggaacagttccgtggctttctgcgtagcctgatgatgcatgccggtgtcggcactgcattaggcggcgtgatgaccatggtgggcgaaccacagaacctgatcatcgctaaagcggctggctggcattttggcgatttcttcctgcgcatgtcgccggtgaccgttccggttctgatttgtggcctgttaacctgcctgctggtagagaagctgcgttggtttggctacggtgaaacgctgccggagaaagtccgcgaagtgttgcaacagtttgacgatcaaagccgccaccagcgtacccgtcaggataaaatccgtctgattgtccaggcgattattggcgtctggctggtgactgcgctggcgttgcatctggcggaagttggcttgattggtttgtcagtcattattctggcgacatcattgaccggtgtcaccgatgaacatgctatcggtaaagccttcaccgaatctctgccattcaccgcactgttgacggtgtttttctcggtagtcgcggtgattatcgaccaacaactgttttcgccaattattcagtttgtgttgcaggcatcggaacatgctcagctgtcgctgttctatattttcaacggtctgctgtcatccatttcggataacgtcttcgtggggacgatttatatcaacgaagcgaaagcggcaatggaaagtggcgctatcacgttgaagcaatacgagctgctggcggtcgccattaataccggtaccaatctgccctccgtcgctacgccgaacggtcaggctgcgttcctgttcctgctgacctctgcactcgcgccattgattcgcctctcttatggccgcatggtgtggatggccctgccttacaccctcgtactgacactcgtcggcttgctctgcgtcgagtttacgcttgcccctgtaaccgaatggtttatgcaaatgggctggatagcaacgctttga BBa_B0034_sequence 1 aaagaggagaaa BBa_K1675005_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatggagatctcctggggccgcgcgctatggcgcaattttttgggccagtcccccgactggtacaaactcgccctcatcattttcttaatcgtaaacccgttaattttcctcatcagccctttcgtcgcgggctggttgctggtagcggaatttattttcactctggcgatggccctgaaatgctacccgctgctccccggtggtctgttggctatcgaagcggtattcatcggcatgaccagcgcggaacacgtccgtgaagaggtggcggcaaatcttgaagtcttgctgttactgatgtttatggtggcgggtatctattttatgaaacagctattgctgttcatatttacccgtttgctgttaagcattcgctccaaaatgctgctgtcgctctctttttgcgtggcggcggcgttcctctccgcgttcctcgatgccttaaccgtcgtggcggtggtgatcagcgttgcagtcggtttttatggtatttatcaccgcgtagcctcttcccgtactgaagataccgacctgcaagacgatagtcatatcgacaagcattacaaagtggttctggaacagttccgtggctttctgcgtagcctgatgatgcatgccggtgtcggcactgcattaggcggcgtgatgaccatggtgggcgaaccacagaacctgatcatcgctaaagcggctggctggcattttggcgatttcttcctgcgcatgtcgccggtgaccgttccggttctgatttgtggcctgttaacctgcctgctggtagagaagctgcgttggtttggctacggtgaaacgctgccggagaaagtccgcgaagtgttgcaacagtttgacgatcaaagccgccaccagcgtacccgtcaggataaaatccgtctgattgtccaggcgattattggcgtctggctggtgactgcgctggcgttgcatctggcggaagttggcttgattggtttgtcagtcattattctggcgacatcattgaccggtgtcaccgatgaacatgctatcggtaaagccttcaccgaatctctgccattcaccgcactgttgacggtgtttttctcggtagtcgcggtgattatcgaccaacaactgttttcgccaattattcagtttgtgttgcaggcatcggaacatgctcagctgtcgctgttctatattttcaacggtctgctgtcatccatttcggataacgtcttcgtggggacgatttatatcaacgaagcgaaagcggcaatggaaagtggcgctatcacgttgaagcaatacgagctgctggcggtcgccattaataccggtaccaatctgccctccgtcgctacgccgaacggtcaggctgcgttcctgttcctgctgacctctgcactcgcgccattgattcgcctctcttatggccgcatggtgtggatggccctgccttacaccctcgtactgacactcgtcggcttgctctgcgtcgagtttacgcttgcccctgtaaccgaatggtttatgcaaatgggctggatagcaacgctttga BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z