BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1675005
1
BBa_K1675005
J23119+B0034+NhaB(BBa_K1675001)
2015-09-09T11:00:00Z
2015-09-12T12:25:21Z
The sequence of promoter J23119 is found through the standard part BBa_J23119 http://parts.igem.org/Part:BBa_J23119
The sequence of ribosome binding site B0034 is found through the standard part BBa_B0034 http://parts.igem.org/Part:BBa_B0034
The sequence of gene NhaB is found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=1233176&to=1234717&strand=true
The constitutive promoter J23119, the ribosome binding site B0034 and the functional gene nhaB were used in this new standard part. This part is available for the bacteria to survive in alkaline environment.
false
false
_2093_
27740
27740
9
false
mutation of CTG to CTA Leu.(site:383bp); mutation of GCT to GCG Ala.(site:461bp); mutation of ACC to ACT Thr.(site:566bp);
mutation of GAC to GAT Asp.(site:572bp); mutation of ACC to ACT Thr.(site:677bp); mutation of GTA to GTT Val.(site:692bp); mutation of GCA to GCG Ala.(site:1040bp); mutation of GAG to GAA Glu.(site:1067bp); mutation of GTC to GTA Val.(site:1499bp)
false
JIAQI XU
component2452860
1
BBa_B0034
component2452858
1
BBa_J23119
component2452873
1
BBa_K1675001
annotation2452858
1
BBa_J23119
range2452858
1
1
35
annotation2452860
1
BBa_B0034
range2452860
1
44
55
annotation2452873
1
BBa_K1675001
range2452873
1
62
1603
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_K1675001
1
BBa_K1675001
NhaB; sodium-proton antiporter; membrane protein
2015-09-08T11:00:00Z
2015-09-15T09:57:31Z
Escherichia coli str. K-12 substr. Sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=fasta&from=1233176&to=1234717&strand=true
The gene, NhaB, is the archetypal sodium-proton antiporter in E. coli. It is crucial when the level of NhaA activity is growth limiting, when NhaA is not sufficiently induced, or when NhaA is not activated.
NhaA catalyzes the exchange of 3 proton ion per 2 sodium ion. Therefore, NhaA could make sure E. coli???s survival under alkaline pH, which are in desperate need in our project.
false
false
_2093_
27740
27740
9
false
mutation of CTG to CTA Leu.(site:336bp); mutation of GCT to GCG Ala.(site:414bp); mutation of ACC to ACT Thr.(site:519bp); mutation of GAC to GAT Asp.(site:525bp); mutation of ACC to ACT Thr.(site:630bp); mutation of GTA to GTG Val.(site:645bp); mutation of GCA to GCG Ala.(site:993bp); mutation of GAG to GAA Glu.(site:1020bp); mutation of GTC to GTA Val.(site:1452bp)
false
JIAQI XU
annotation2456965
1
NhaB Coding Region
range2456965
1
1
1542
annotation2456967
1
TGA stop codon
range2456967
1
1540
1542
annotation2456966
1
ATG start codon
range2456966
1
1
3
BBa_K1675001_sequence
1
atggagatctcctggggccgcgcgctatggcgcaattttttgggccagtcccccgactggtacaaactcgccctcatcattttcttaatcgtaaacccgttaattttcctcatcagccctttcgtcgcgggctggttgctggtagcggaatttattttcactctggcgatggccctgaaatgctacccgctgctccccggtggtctgttggctatcgaagcggtattcatcggcatgaccagcgcggaacacgtccgtgaagaggtggcggcaaatcttgaagtcttgctgttactgatgtttatggtggcgggtatctattttatgaaacagctattgctgttcatatttacccgtttgctgttaagcattcgctccaaaatgctgctgtcgctctctttttgcgtggcggcggcgttcctctccgcgttcctcgatgccttaaccgtcgtggcggtggtgatcagcgttgcagtcggtttttatggtatttatcaccgcgtagcctcttcccgtactgaagataccgacctgcaagacgatagtcatatcgacaagcattacaaagtggttctggaacagttccgtggctttctgcgtagcctgatgatgcatgccggtgtcggcactgcattaggcggcgtgatgaccatggtgggcgaaccacagaacctgatcatcgctaaagcggctggctggcattttggcgatttcttcctgcgcatgtcgccggtgaccgttccggttctgatttgtggcctgttaacctgcctgctggtagagaagctgcgttggtttggctacggtgaaacgctgccggagaaagtccgcgaagtgttgcaacagtttgacgatcaaagccgccaccagcgtacccgtcaggataaaatccgtctgattgtccaggcgattattggcgtctggctggtgactgcgctggcgttgcatctggcggaagttggcttgattggtttgtcagtcattattctggcgacatcattgaccggtgtcaccgatgaacatgctatcggtaaagccttcaccgaatctctgccattcaccgcactgttgacggtgtttttctcggtagtcgcggtgattatcgaccaacaactgttttcgccaattattcagtttgtgttgcaggcatcggaacatgctcagctgtcgctgttctatattttcaacggtctgctgtcatccatttcggataacgtcttcgtggggacgatttatatcaacgaagcgaaagcggcaatggaaagtggcgctatcacgttgaagcaatacgagctgctggcggtcgccattaataccggtaccaatctgccctccgtcgctacgccgaacggtcaggctgcgttcctgttcctgctgacctctgcactcgcgccattgattcgcctctcttatggccgcatggtgtggatggccctgccttacaccctcgtactgacactcgtcggcttgctctgcgtcgagtttacgcttgcccctgtaaccgaatggtttatgcaaatgggctggatagcaacgctttga
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1675005_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatggagatctcctggggccgcgcgctatggcgcaattttttgggccagtcccccgactggtacaaactcgccctcatcattttcttaatcgtaaacccgttaattttcctcatcagccctttcgtcgcgggctggttgctggtagcggaatttattttcactctggcgatggccctgaaatgctacccgctgctccccggtggtctgttggctatcgaagcggtattcatcggcatgaccagcgcggaacacgtccgtgaagaggtggcggcaaatcttgaagtcttgctgttactgatgtttatggtggcgggtatctattttatgaaacagctattgctgttcatatttacccgtttgctgttaagcattcgctccaaaatgctgctgtcgctctctttttgcgtggcggcggcgttcctctccgcgttcctcgatgccttaaccgtcgtggcggtggtgatcagcgttgcagtcggtttttatggtatttatcaccgcgtagcctcttcccgtactgaagataccgacctgcaagacgatagtcatatcgacaagcattacaaagtggttctggaacagttccgtggctttctgcgtagcctgatgatgcatgccggtgtcggcactgcattaggcggcgtgatgaccatggtgggcgaaccacagaacctgatcatcgctaaagcggctggctggcattttggcgatttcttcctgcgcatgtcgccggtgaccgttccggttctgatttgtggcctgttaacctgcctgctggtagagaagctgcgttggtttggctacggtgaaacgctgccggagaaagtccgcgaagtgttgcaacagtttgacgatcaaagccgccaccagcgtacccgtcaggataaaatccgtctgattgtccaggcgattattggcgtctggctggtgactgcgctggcgttgcatctggcggaagttggcttgattggtttgtcagtcattattctggcgacatcattgaccggtgtcaccgatgaacatgctatcggtaaagccttcaccgaatctctgccattcaccgcactgttgacggtgtttttctcggtagtcgcggtgattatcgaccaacaactgttttcgccaattattcagtttgtgttgcaggcatcggaacatgctcagctgtcgctgttctatattttcaacggtctgctgtcatccatttcggataacgtcttcgtggggacgatttatatcaacgaagcgaaagcggcaatggaaagtggcgctatcacgttgaagcaatacgagctgctggcggtcgccattaataccggtaccaatctgccctccgtcgctacgccgaacggtcaggctgcgttcctgttcctgctgacctctgcactcgcgccattgattcgcctctcttatggccgcatggtgtggatggccctgccttacaccctcgtactgacactcgtcggcttgctctgcgtcgagtttacgcttgcccctgtaaccgaatggtttatgcaaatgggctggatagcaacgctttga
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z