LOCUS BBa_K1675006 2798 bp DNA linear 22-Nov-2024 DEFINITION J23119+B0034+NhaA+B0034+NhaB ACCESSION BBa_K1675006 VERSION BBa_K1675006.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23119 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 64..66 /label=GTG Start Codon CDS 64..1230 /label=NhaA Coding Region misc_feature 1228..1230 /label=TGA Stop Codon CDS 64..1230 /label=BBa_K1675000 misc_feature 1243..1246 /label=conserved RBS 1239..1250 /label=BBa_B0034 CDS 1257..2798 /label=NhaB Coding Region misc_feature 1257..1259 /label=ATG start codon misc_feature 2796..2798 /label=TGA stop codon CDS 1257..2798 /label=BBa_K1675001 ORIGIN 1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta 61 gaggtgaaac atctgcatcg attctttagc agtgatgcct cgggaggcat tattcttatc 121 attgccgcta tcctggcgat gattatggcc aacagcggcg caaccagtgg atggtatcac 181 gactttctgg agacgccggt tcagctccgg gttggttcac tcgaaatcaa caaaaacatg 241 ctgttatgga taaatgacgc gctgatggcg gtatttttcc tgttagtcgg tctggaagtt 301 aaacgtgaac tgatgcaagg atcgctagcc agcttacgcc aggccgcatt tccagttatc 361 gccgctattg gtgggatgat tgtgccggca ttactctatc tggcttttaa ctatgccgat 421 ccgattaccc gcgaagggtg ggcgatcccg gcggctactg acattgcttt tgcacttggt 481 gtactggcgc tgttgggaag tcgtgttccg ttagcgctga agatcttttt gatggctctg 541 gctattatcg acgatcttgg ggccatcatt atcatcgcat tgttctacac taatgactta 601 tcgatggcct ctcttggcgt cgcggctgta gcaattgcgg tactcgcggt attgaatctg 661 tgtggtgcac gccgcacggg cgtctatatt cttgttggcg tggtgttgtg gactgcggtg 721 ttgaaatcgg gggttcacgc aactctggcg ggggtaattg tcggcttctt tattcctttg 781 aaagagaagc atgggcgttc tccagcgaag cgactggagc atgtgttgca cccgtgggtg 841 gcgtatctga ttttgccgct gtttgcattt gctaatgctg gcgtttcact gcaaggcgtc 901 acgctggatg gcttgacctc cattctgcca ttggggatca tcgctggctt gctgattggc 961 aaaccgctgg ggattagtct gttctgctgg ttggcgctgc gtttgaaact ggcgcatctg 1021 cctgagggaa cgacttatca gcaaattatg gtggtgggga tcctgtgcgg tatcggtttt 1081 actatgtcta tctttattgc cagcctggcc tttggtagcg tagatccaga actgattaac 1141 tgggcgaaac tcggtatcct ggtcggttct atctcttcgg cggtaattgg atacagctgg 1201 ttacgcgttc gtttgcgtcc atcagtttga tactagagaa agaggagaaa tactagatgg 1261 agatctcctg gggccgcgcg ctatggcgca attttttggg ccagtccccc gactggtaca 1321 aactcgccct catcattttc ttaatcgtaa acccgttaat tttcctcatc agccctttcg 1381 tcgcgggctg gttgctggta gcggaattta ttttcactct ggcgatggcc ctgaaatgct 1441 acccgctgct ccccggtggt ctgttggcta tcgaagcggt attcatcggc atgaccagcg 1501 cggaacacgt ccgtgaagag gtggcggcaa atcttgaagt cttgctgtta ctgatgttta 1561 tggtggcggg tatctatttt atgaaacagc tattgctgtt catatttacc cgtttgctgt 1621 taagcattcg ctccaaaatg ctgctgtcgc tctctttttg cgtggcggcg gcgttcctct 1681 ccgcgttcct cgatgcctta accgtcgtgg cggtggtgat cagcgttgca gtcggttttt 1741 atggtattta tcaccgcgta gcctcttccc gtactgaaga taccgacctg caagacgata 1801 gtcatatcga caagcattac aaagtggttc tggaacagtt ccgtggcttt ctgcgtagcc 1861 tgatgatgca tgccggtgtc ggcactgcat taggcggcgt gatgaccatg gtgggcgaac 1921 cacagaacct gatcatcgct aaagcggctg gctggcattt tggcgatttc ttcctgcgca 1981 tgtcgccggt gaccgttccg gttctgattt gtggcctgtt aacctgcctg ctggtagaga 2041 agctgcgttg gtttggctac ggtgaaacgc tgccggagaa agtccgcgaa gtgttgcaac 2101 agtttgacga tcaaagccgc caccagcgta cccgtcagga taaaatccgt ctgattgtcc 2161 aggcgattat tggcgtctgg ctggtgactg cgctggcgtt gcatctggcg gaagttggct 2221 tgattggttt gtcagtcatt attctggcga catcattgac cggtgtcacc gatgaacatg 2281 ctatcggtaa agccttcacc gaatctctgc cattcaccgc actgttgacg gtgtttttct 2341 cggtagtcgc ggtgattatc gaccaacaac tgttttcgcc aattattcag tttgtgttgc 2401 aggcatcgga acatgctcag ctgtcgctgt tctatatttt caacggtctg ctgtcatcca 2461 tttcggataa cgtcttcgtg gggacgattt atatcaacga agcgaaagcg gcaatggaaa 2521 gtggcgctat cacgttgaag caatacgagc tgctggcggt cgccattaat accggtacca 2581 atctgccctc cgtcgctacg ccgaacggtc aggctgcgtt cctgttcctg ctgacctctg 2641 cactcgcgcc attgattcgc ctctcttatg gccgcatggt gtggatggcc ctgccttaca 2701 ccctcgtact gacactcgtc ggcttgctct gcgtcgagtt tacgcttgcc cctgtaaccg 2761 aatggtttat gcaaatgggc tggatagcaa cgctttga //