BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1675006 1 BBa_K1675006 J23119+B0034+NhaA+B0034+NhaB 2015-09-10T11:00:00Z 2015-09-12T12:51:52Z The sequence of promoter J23119 is found through the standard part BBa_J23119 http://parts.igem.org/Part:BBa_J23119 The sequence of ribosome binding site B0034 is found through the standard part BBa_B0034 http://parts.igem.org/Part:BBa_B0034 The sequence of gene NhaA is found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=17489&to=18655 The sequence of gene NhaB is found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=1233176&to=1234717&strand=true Construct the JBA (J23119+B0034+NhaA), B0034 and NhaB with the vector pSB1C3. Two functional genes NhaA and NhaB are used to pump in protons and lower the pH of bacteria???s microenvironment. JBA and JBB are linked with pSB1C3 through 3A Assemble successfully. Thus, it can be an alkaline-resisting part. false false _2093_ 27740 27740 9 false This is a composite part including the J23119 promoter ecoded in part BBa_J23119 and the ribosome binding site B0034 ecoded in part BBa_B0034. The functional genes NhaA and NhaB are cloned from Escherichia coli str. K-12 substr. sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=17489&to=18655 http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=1233176&to=1234717&strand=true false JIAQI XU component2449070 1 BBa_B0034 component2449068 1 BBa_K1675000 component2449064 1 BBa_B0034 component2449083 1 BBa_K1675001 component2449062 1 BBa_J23119 annotation2449062 1 BBa_J23119 range2449062 1 1 35 annotation2449083 1 BBa_K1675001 range2449083 1 1257 2798 annotation2449070 1 BBa_B0034 range2449070 1 1239 1250 annotation2449064 1 BBa_B0034 range2449064 1 44 55 annotation2449068 1 BBa_K1675000 range2449068 1 64 1230 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_K1675000 1 BBa_K1675000 NhaA; sodion-proton antiporter; membrane protein 2015-09-08T11:00:00Z 2015-09-18T11:49:20Z Escherichia coli str. K-12 substr. Sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=17489&to=18655 The gene,NhaA, is the archetypal sodium-proton antiporter in E. coli. It is the only member of the family that is absolutely required by E. coli for survival in high-salt conditions, under alkaline stress, or in the presence of otherwise toxic Li+ concentration. NhaA catalyzes the exchange of 2 H+ per Na+. Therefore, nhaA could make sure E. coli???s survival under alkaline pH, which are in desperate need in our project. false false _2093_ 27740 27740 9 false None. false JIAQI XU annotation2447758 1 TGA Stop Codon range2447758 1 1165 1167 annotation2447757 1 NhaA Coding Region range2447757 1 1 1167 annotation2447756 1 GTG Start Codon range2447756 1 1 3 BBa_K1675001 1 BBa_K1675001 NhaB; sodium-proton antiporter; membrane protein 2015-09-08T11:00:00Z 2015-09-15T09:57:31Z Escherichia coli str. K-12 substr. Sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=fasta&from=1233176&to=1234717&strand=true The gene, NhaB, is the archetypal sodium-proton antiporter in E. coli. It is crucial when the level of NhaA activity is growth limiting, when NhaA is not sufficiently induced, or when NhaA is not activated. NhaA catalyzes the exchange of 3 proton ion per 2 sodium ion. Therefore, NhaA could make sure E. coli???s survival under alkaline pH, which are in desperate need in our project. false false _2093_ 27740 27740 9 false mutation of CTG to CTA Leu.(site:336bp); mutation of GCT to GCG Ala.(site:414bp); mutation of ACC to ACT Thr.(site:519bp); mutation of GAC to GAT Asp.(site:525bp); mutation of ACC to ACT Thr.(site:630bp); mutation of GTA to GTG Val.(site:645bp); mutation of GCA to GCG Ala.(site:993bp); mutation of GAG to GAA Glu.(site:1020bp); mutation of GTC to GTA Val.(site:1452bp) false JIAQI XU annotation2456965 1 NhaB Coding Region range2456965 1 1 1542 annotation2456967 1 TGA stop codon range2456967 1 1540 1542 annotation2456966 1 ATG start codon range2456966 1 1 3 BBa_K1675001_sequence 1 atggagatctcctggggccgcgcgctatggcgcaattttttgggccagtcccccgactggtacaaactcgccctcatcattttcttaatcgtaaacccgttaattttcctcatcagccctttcgtcgcgggctggttgctggtagcggaatttattttcactctggcgatggccctgaaatgctacccgctgctccccggtggtctgttggctatcgaagcggtattcatcggcatgaccagcgcggaacacgtccgtgaagaggtggcggcaaatcttgaagtcttgctgttactgatgtttatggtggcgggtatctattttatgaaacagctattgctgttcatatttacccgtttgctgttaagcattcgctccaaaatgctgctgtcgctctctttttgcgtggcggcggcgttcctctccgcgttcctcgatgccttaaccgtcgtggcggtggtgatcagcgttgcagtcggtttttatggtatttatcaccgcgtagcctcttcccgtactgaagataccgacctgcaagacgatagtcatatcgacaagcattacaaagtggttctggaacagttccgtggctttctgcgtagcctgatgatgcatgccggtgtcggcactgcattaggcggcgtgatgaccatggtgggcgaaccacagaacctgatcatcgctaaagcggctggctggcattttggcgatttcttcctgcgcatgtcgccggtgaccgttccggttctgatttgtggcctgttaacctgcctgctggtagagaagctgcgttggtttggctacggtgaaacgctgccggagaaagtccgcgaagtgttgcaacagtttgacgatcaaagccgccaccagcgtacccgtcaggataaaatccgtctgattgtccaggcgattattggcgtctggctggtgactgcgctggcgttgcatctggcggaagttggcttgattggtttgtcagtcattattctggcgacatcattgaccggtgtcaccgatgaacatgctatcggtaaagccttcaccgaatctctgccattcaccgcactgttgacggtgtttttctcggtagtcgcggtgattatcgaccaacaactgttttcgccaattattcagtttgtgttgcaggcatcggaacatgctcagctgtcgctgttctatattttcaacggtctgctgtcatccatttcggataacgtcttcgtggggacgatttatatcaacgaagcgaaagcggcaatggaaagtggcgctatcacgttgaagcaatacgagctgctggcggtcgccattaataccggtaccaatctgccctccgtcgctacgccgaacggtcaggctgcgttcctgttcctgctgacctctgcactcgcgccattgattcgcctctcttatggccgcatggtgtggatggccctgccttacaccctcgtactgacactcgtcggcttgctctgcgtcgagtttacgcttgcccctgtaaccgaatggtttatgcaaatgggctggatagcaacgctttga BBa_B0034_sequence 1 aaagaggagaaa BBa_K1675000_sequence 1 gtgaaacatctgcatcgattctttagcagtgatgcctcgggaggcattattcttatcattgccgctatcctggcgatgattatggccaacagcggcgcaaccagtggatggtatcacgactttctggagacgccggttcagctccgggttggttcactcgaaatcaacaaaaacatgctgttatggataaatgacgcgctgatggcggtatttttcctgttagtcggtctggaagttaaacgtgaactgatgcaaggatcgctagccagcttacgccaggccgcatttccagttatcgccgctattggtgggatgattgtgccggcattactctatctggcttttaactatgccgatccgattacccgcgaagggtgggcgatcccggcggctactgacattgcttttgcacttggtgtactggcgctgttgggaagtcgtgttccgttagcgctgaagatctttttgatggctctggctattatcgacgatcttggggccatcattatcatcgcattgttctacactaatgacttatcgatggcctctcttggcgtcgcggctgtagcaattgcggtactcgcggtattgaatctgtgtggtgcacgccgcacgggcgtctatattcttgttggcgtggtgttgtggactgcggtgttgaaatcgggggttcacgcaactctggcgggggtaattgtcggcttctttattcctttgaaagagaagcatgggcgttctccagcgaagcgactggagcatgtgttgcacccgtgggtggcgtatctgattttgccgctgtttgcatttgctaatgctggcgtttcactgcaaggcgtcacgctggatggcttgacctccattctgccattggggatcatcgctggcttgctgattggcaaaccgctggggattagtctgttctgctggttggcgctgcgtttgaaactggcgcatctgcctgagggaacgacttatcagcaaattatggtggtggggatcctgtgcggtatcggttttactatgtctatctttattgccagcctggcctttggtagcgtagatccagaactgattaactgggcgaaactcggtatcctggtcggttctatctcttcggcggtaattggatacagctggttacgcgttcgtttgcgtccatcagtttga BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc BBa_K1675006_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagaggtgaaacatctgcatcgattctttagcagtgatgcctcgggaggcattattcttatcattgccgctatcctggcgatgattatggccaacagcggcgcaaccagtggatggtatcacgactttctggagacgccggttcagctccgggttggttcactcgaaatcaacaaaaacatgctgttatggataaatgacgcgctgatggcggtatttttcctgttagtcggtctggaagttaaacgtgaactgatgcaaggatcgctagccagcttacgccaggccgcatttccagttatcgccgctattggtgggatgattgtgccggcattactctatctggcttttaactatgccgatccgattacccgcgaagggtgggcgatcccggcggctactgacattgcttttgcacttggtgtactggcgctgttgggaagtcgtgttccgttagcgctgaagatctttttgatggctctggctattatcgacgatcttggggccatcattatcatcgcattgttctacactaatgacttatcgatggcctctcttggcgtcgcggctgtagcaattgcggtactcgcggtattgaatctgtgtggtgcacgccgcacgggcgtctatattcttgttggcgtggtgttgtggactgcggtgttgaaatcgggggttcacgcaactctggcgggggtaattgtcggcttctttattcctttgaaagagaagcatgggcgttctccagcgaagcgactggagcatgtgttgcacccgtgggtggcgtatctgattttgccgctgtttgcatttgctaatgctggcgtttcactgcaaggcgtcacgctggatggcttgacctccattctgccattggggatcatcgctggcttgctgattggcaaaccgctggggattagtctgttctgctggttggcgctgcgtttgaaactggcgcatctgcctgagggaacgacttatcagcaaattatggtggtggggatcctgtgcggtatcggttttactatgtctatctttattgccagcctggcctttggtagcgtagatccagaactgattaactgggcgaaactcggtatcctggtcggttctatctcttcggcggtaattggatacagctggttacgcgttcgtttgcgtccatcagtttgatactagagaaagaggagaaatactagatggagatctcctggggccgcgcgctatggcgcaattttttgggccagtcccccgactggtacaaactcgccctcatcattttcttaatcgtaaacccgttaattttcctcatcagccctttcgtcgcgggctggttgctggtagcggaatttattttcactctggcgatggccctgaaatgctacccgctgctccccggtggtctgttggctatcgaagcggtattcatcggcatgaccagcgcggaacacgtccgtgaagaggtggcggcaaatcttgaagtcttgctgttactgatgtttatggtggcgggtatctattttatgaaacagctattgctgttcatatttacccgtttgctgttaagcattcgctccaaaatgctgctgtcgctctctttttgcgtggcggcggcgttcctctccgcgttcctcgatgccttaaccgtcgtggcggtggtgatcagcgttgcagtcggtttttatggtatttatcaccgcgtagcctcttcccgtactgaagataccgacctgcaagacgatagtcatatcgacaagcattacaaagtggttctggaacagttccgtggctttctgcgtagcctgatgatgcatgccggtgtcggcactgcattaggcggcgtgatgaccatggtgggcgaaccacagaacctgatcatcgctaaagcggctggctggcattttggcgatttcttcctgcgcatgtcgccggtgaccgttccggttctgatttgtggcctgttaacctgcctgctggtagagaagctgcgttggtttggctacggtgaaacgctgccggagaaagtccgcgaagtgttgcaacagtttgacgatcaaagccgccaccagcgtacccgtcaggataaaatccgtctgattgtccaggcgattattggcgtctggctggtgactgcgctggcgttgcatctggcggaagttggcttgattggtttgtcagtcattattctggcgacatcattgaccggtgtcaccgatgaacatgctatcggtaaagccttcaccgaatctctgccattcaccgcactgttgacggtgtttttctcggtagtcgcggtgattatcgaccaacaactgttttcgccaattattcagtttgtgttgcaggcatcggaacatgctcagctgtcgctgttctatattttcaacggtctgctgtcatccatttcggataacgtcttcgtggggacgatttatatcaacgaagcgaaagcggcaatggaaagtggcgctatcacgttgaagcaatacgagctgctggcggtcgccattaataccggtaccaatctgccctccgtcgctacgccgaacggtcaggctgcgttcctgttcctgctgacctctgcactcgcgccattgattcgcctctcttatggccgcatggtgtggatggccctgccttacaccctcgtactgacactcgtcggcttgctctgcgtcgagtttacgcttgcccctgtaaccgaatggtttatgcaaatgggctggatagcaacgctttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z