BBa_K1675008 1 BBa_K1675008 A device used in the synthesis of lactic acid 2015-09-14T11:00:00Z 2015-09-15T12:22:50Z LdhA is from the genome of E.coli K-12.http://www.ncbi.nlm.nih.gov/gene/946315 P-atp2 is a kind of mutant alkali-induced promoter. The natural sequence of P-atp2 is from the standard part BBa_K1170003. http://parts.igem.org/Part:BBa_K1170003:Design The RBS is from the standard part BBa_B0034. http://parts.igem.org/Part:BBa_B0034 L-Lactic acid, one of the most important chiral molecules and organic acids, is produced via pyruvate from carbohydrates in diverse microorganisms catalyzed by an NAD+-dependent L-lactate dehydrogenase. The LdhA gene is used in the synthesis of lactic acid. This gene encodes an L-lactate dehydrogenase (L-LDH) which converts pyruvate to L-lactate. false false _2093_ 27740 27740 9 false None. false JIAQI XU component2457374 1 BBa_B0034 component2457372 1 BBa_K1675016 component2457375 1 BBa_K1154003 annotation2457375 1 BBa_K1154003 range2457375 1 634 1632 annotation2457372 1 BBa_K1675016 range2457372 1 1 607 annotation2457374 1 BBa_B0034 range2457374 1 616 627 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1675016 1 BBa_K1675016 P-atp2 mutant 140-B0034-lacz alpha 2015-09-14T11:00:00Z 2015-09-15T11:58:16Z Error prone PCR of P-atp2, then overlap extension of synthetic oligonucleotides. The natural P-atp2 is from Corynebacterium glutamicum. Lacz alpha is from http://parts.igem.org/wiki/index.php?title=Part:BBa_I732006 The natural P-atp2 is an alkali-induced promoter in Corynebacterium glutamicum located in F0F1 ATPase operon. The natural P-atp2 promoter responds to the pH changes from pH 7.0 to pH 9.0, especially at alkaline pH. When the pH value increasing, the expression increased modestly. It is activated by the alternative sigma factor of the RNA polymerase, whose synthesis would be activated when the bacteria are growing at alkaline pH. The activity of this mutant at different pH is shown in figure 1. false false _2093_ 28109 28109 9 false There is an EcoRI site exists in the P-atp2 sequence, so we mutate it to change it. false Jing Yang BBa_K1154003 1 LDHA Homo sapiens lactate dehydrogenase A (LDHA) 2013-09-16T11:00:00Z 2015-05-08T01:09:29Z Synthetic coding sequence is derived from the polypeptide sequence of Homo sapiens lactate dehydrogenase subunit A. Subunit A of L-lactate-specific dehydrogenase (M-isozyme) catalyzes the interconversion of pyruvate plus NADH and L-lactate plus NAD in the final step of anaerobic glycolysis. false false _1466_ 0 12086 9 Not in stock false The sequence is optimized for high codon bias in yeast and conformity with current BioBrick standards. false Adam Nighswander, Tanner Reeb and Bianca Maled BBa_B0034_sequence 1 aaagaggagaaa BBa_K1675016_sequence 1 agtgtccgtgctgggaaactggcggggaacttttagagataaccctccgaattgctggcaaatttctgatgaaatttctccgcgaagcccacatgaactacccccgtttaccctcaaaataagccctgtgacacacataacaccccctaatcgtacccgctcacacgctattttcgaggtgtggttcgccttcggaaacgaatgcccccgccccacttggataaaagacgttaacacctgttagtctataacgcgggttgaaccgagaaacccctcaaggcagcagacaatagccgcaaggggttttgcggagcacgtcccctgtgatcgttgcgctgatgtgcgacggagtccgaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataa BBa_K1675008_sequence 1 agtgtccgtgctgggaaactggcggggaacttttagagataaccctccgaattgctggcaaatttctgatgaaatttctccgcgaagcccacatgaactacccccgtttaccctcaaaataagccctgtgacacacataacaccccctaatcgtacccgctcacacgctattttcgaggtgtggttcgccttcggaaacgaatgcccccgccccacttggataaaagacgttaacacctgttagtctataacgcgggttgaaccgagaaacccctcaaggcagcagacaatagccgcaaggggttttgcggagcacgtcccctgtgatcgttgcgctgatgtgcgacggagtccgaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataatactagagaaagaggagaaatactagatggctactttgaaagatcaattgatttataatttgttgaaagaagaacaaactccacaaaataaaattactgttgttggtgttggtgctgttggtatggcttgtgctatttctattttgatgaaagatttggctgatgaattggctttggttgatgttattgaagataaattgaaaggtgaaatgatggatttgcaacatggttctttgtttttgagaactccaaaaattgtttctggtaaagattataatgttactgctaattctaaattggttattattactgctggtgctagacaacaagaaggtgaatcaagattgaatttggttcaaagaaatgttaatatttttaaatttattattccaaatgttgttaaatattctccaaattgtaaattgttgattgtttctaatccagttgatattttgacttatgttgcttggaaaatttctggttttccaaaaaatagagttattggttctggttgtaatttggattctgctagatttagatatttgatgggtgaaagattgggtgttcatccattgtcttgtcatggttgggttttgggtgaacatggtgattcttctgttccagtttggtctggtatgaatgttgctggtgtttctttgaaaactttgcatccagatttgggtactgataaagataaagaacaatggaaagaagttcataaacaagttgttgaatctgcttatgaagttattaaattgaaaggttatacttcttgggctattggtttgtctgttgctgatttggctgaatctattatgaaaaatttgagaagagttcatccagtttctactatgattaaaggtttgtatggtattaaagatgatgtttttttgtctgttccatgtattttgggtcaaaatggtatttctgatttggttaaagttactttgacttctgaagaagaagctagattgaaaaaatctgctgatactttgtggggtattcaaaaagaattgcaattttaa BBa_K1154003_sequence 1 atggctactttgaaagatcaattgatttataatttgttgaaagaagaacaaactccacaaaataaaattactgttgttggtgttggtgctgttggtatggcttgtgctatttctattttgatgaaagatttggctgatgaattggctttggttgatgttattgaagataaattgaaaggtgaaatgatggatttgcaacatggttctttgtttttgagaactccaaaaattgtttctggtaaagattataatgttactgctaattctaaattggttattattactgctggtgctagacaacaagaaggtgaatcaagattgaatttggttcaaagaaatgttaatatttttaaatttattattccaaatgttgttaaatattctccaaattgtaaattgttgattgtttctaatccagttgatattttgacttatgttgcttggaaaatttctggttttccaaaaaatagagttattggttctggttgtaatttggattctgctagatttagatatttgatgggtgaaagattgggtgttcatccattgtcttgtcatggttgggttttgggtgaacatggtgattcttctgttccagtttggtctggtatgaatgttgctggtgtttctttgaaaactttgcatccagatttgggtactgataaagataaagaacaatggaaagaagttcataaacaagttgttgaatctgcttatgaagttattaaattgaaaggttatacttcttgggctattggtttgtctgttgctgatttggctgaatctattatgaaaaatttgagaagagttcatccagtttctactatgattaaaggtttgtatggtattaaagatgatgtttttttgtctgttccatgtattttgggtcaaaatggtatttctgatttggttaaagttactttgacttctgaagaagaagctagattgaaaaaatctgctgatactttgtggggtattcaaaaagaattgcaattttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z