LOCUS       BBa_K1675009            1605 bp    DNA     linear       22-Dec-2024
DEFINITION  a device to produce alkali and neutralize the external acidic 
            environment
ACCESSION   BBa_K1675009
VERSION     BBa_K1675009.1  
FEATURES             Location/Qualifiers
     promoter        1..178
                     /label=asr promoter
     misc_feature    141..147
                     /label=TATA box
     misc_feature    114..131
                     /label=Pho Box
     promoter        1..178
                     /label=BBa_K1170000
     misc_feature    191..194
                     /label=conserved
     RBS             187..198
                     /label=BBa_B0034
     CDS             205..1605
                     /label=GadA Coding Region
     misc_feature    205..207
                     /label=ATG Start Codon
     misc_feature    1603..1605
                     /label=TGA Stop Codon
     CDS             205..1605
                     /label=BBa_K1675003
ORIGIN
        1 cgatcaagac tactattatt ggtagctaaa tttcccttaa gtcacaatac gttattatca
       61 acgctgtaat ttattcagcg tttgtacata tcgttacacg ctgaaaccaa ccactcacgg
      121 aagtctgcca ttcccaggga tatagttatt tcaacggccc cgcagtgggg ttaaatgata
      181 ctagagaaag aggagaaata ctagatggac cagaagctgt taacggattt ccgctcagaa
      241 ctactcgatt cacgttttgg cgcaaaggcc atttctacta tcgcggagtc aaaacgattt
      301 ccgctgcacg aaatgcgcga tgatgtcgca tttcagatta tcaatgatga attatatctt
      361 gatggcaacg ctcgtcagaa cctggccact ttctgccaga cctgggacga cgaaaacgtc
      421 cataaattga tggatttgtc gatcaataaa aactggatcg acaaagaaga atatccgcaa
      481 tccgcagcca tcgacctgcg ttgcgtaaat atggttgccg atctgtggca tgcgcctgcg
      541 ccgaaaaatg gtcaggccgt tggcaccaac accattggtt cttccgaggc ctgtatgctc
      601 ggcgggatgg cgatgaaatg gcgttggcgc aagcgtatgg aagctgcggg caaaccaacg
      661 gataaaccaa acctggtgtg cggtccggta caaatctgct ggcataaatt cgcccgctac
      721 tgggatgtgg agctgcgtga gatccctatg cgccccggtc agttgtttat ggacccgaaa
      781 cgcatgattg aagcctgtga cgaaaacacc atcggcgtgg tgccgacttt cggcgtgacc
      841 tacaccggta actatgagtt cccacaaccg ctgcacgatg cgctggataa attccaggcc
      901 gacaccggta tcgacatcga catgcacatc gacgctgcca gcggtggctt cctggcaccg
      961 ttcgtcgccc cggatatcgt ctgggacttc cgcctgccgc gtgtgaaatc gatcagtgct
     1021 tcaggccata aattcggtct ggctccgctg ggctgcggct gggttatctg gcgtgacgaa
     1081 gaagcgctgc cgcaggaact ggtgttcaac gttgactacc tgggtggtca aattggtact
     1141 tttgccatca acttctcccg cccggcgggt caggtaattg cacagtacta tgagttcctg
     1201 cgcctcggtc gtgaaggcta taccaaagta cagaacgcct cttaccaggt tgccgcttat
     1261 ctggcggatg aaatcgccaa actggggccg tatgagttca tctgtacggg tcgcccggac
     1321 gaaggcatcc cggcggtttg cttcaaactg aaagatggtg aagatccggg atacaccctg
     1381 tacgacctct ctgaacgtct gcgtctgcgc ggctggcagg ttccggcctt cactctcggc
     1441 ggtgaagcca ccgacatcgt ggtgatgcgc attatgtgtc gtcgcggctt cgaaatggac
     1501 tttgctgaac tgttgctgga agactacaaa gcctccctga aatatctcag cgatcacccg
     1561 aaactgcaag gtattgccca gcagaacagc tttaaacaca cctga
//