BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1170000 1 BBa_K1170000 asr (acid shock response) promoter 2013-09-01T11:00:00Z 2015-05-08T01:09:34Z This part was extracted from the genome of E. coli K12 An acid inducible promoter of the acid shock response (asr) gene, extracted from E. coli K12. This part can be used to generate pH based response in any biological system. false false _1483_ 0 16731 9 Not in stock false The sequence provided in literature indicated a consensus sequence for the Pho Box just before the transcription start site false Rohit Satija annotation2334963 1 TATA box range2334963 1 141 147 annotation2334962 1 asr promoter range2334962 1 1 178 annotation2334964 1 Pho Box range2334964 1 114 131 BBa_K1675003 1 BBa_K1675003 glutamate decarboxylase A &#65288;GadA&#65289; 2015-09-08T11:00:00Z 2015-09-15T09:54:17Z Escherichia coli str. K-12 substr. Sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=3666180&to=3667580&strand=true The gene GadA (glutamate decarboxylase A) can be cloned from Escherichia coli str. K-12 substr. GadA is a functional gene for producing alkali. It catalyzes the proton-consuming conversion of glutamate to &#947;-amino butyric acid (GABA) whereas it converts glutamate to GABA by removing the &#945;-carboxylate group of glutamate. false false _2093_ 27740 27740 9 false Mutation of TTT to TTC Phe.(site:129bp) false JIAQI XU annotation2456981 1 TGA Stop Codon range2456981 1 1399 1401 annotation2456979 1 GadA Coding Region range2456979 1 1 1401 annotation2456980 1 ATG Start Codon range2456980 1 1 3 BBa_K1675009 1 BBa_K1675009 a device to produce alkali and neutralize the external acidic environment 2015-09-11T11:00:00Z 2015-09-27T05:58:31Z the genome of E.coli K-12 Uses an acid induced promoter asr(BBa_K1170000), strong RBS(BBa_B0034),alkaline substance producing gene gadA coding region(BBa_K1675003).Will produce alkali under acidic environment and neutralize the external pH. false false _2093_ 21876 25299 9 false None false Hui Luo component2477845 1 BBa_K1170000 component2477847 1 BBa_B0034 component2477851 1 BBa_K1675003 annotation2477845 1 BBa_K1170000 range2477845 1 1 178 annotation2477851 1 BBa_K1675003 range2477851 1 205 1605 annotation2477847 1 BBa_B0034 range2477847 1 187 198 BBa_K1675009_sequence 1 cgatcaagactactattattggtagctaaatttcccttaagtcacaatacgttattatcaacgctgtaatttattcagcgtttgtacatatcgttacacgctgaaaccaaccactcacggaagtctgccattcccagggatatagttatttcaacggccccgcagtggggttaaatgatactagagaaagaggagaaatactagatggaccagaagctgttaacggatttccgctcagaactactcgattcacgttttggcgcaaaggccatttctactatcgcggagtcaaaacgatttccgctgcacgaaatgcgcgatgatgtcgcatttcagattatcaatgatgaattatatcttgatggcaacgctcgtcagaacctggccactttctgccagacctgggacgacgaaaacgtccataaattgatggatttgtcgatcaataaaaactggatcgacaaagaagaatatccgcaatccgcagccatcgacctgcgttgcgtaaatatggttgccgatctgtggcatgcgcctgcgccgaaaaatggtcaggccgttggcaccaacaccattggttcttccgaggcctgtatgctcggcgggatggcgatgaaatggcgttggcgcaagcgtatggaagctgcgggcaaaccaacggataaaccaaacctggtgtgcggtccggtacaaatctgctggcataaattcgcccgctactgggatgtggagctgcgtgagatccctatgcgccccggtcagttgtttatggacccgaaacgcatgattgaagcctgtgacgaaaacaccatcggcgtggtgccgactttcggcgtgacctacaccggtaactatgagttcccacaaccgctgcacgatgcgctggataaattccaggccgacaccggtatcgacatcgacatgcacatcgacgctgccagcggtggcttcctggcaccgttcgtcgccccggatatcgtctgggacttccgcctgccgcgtgtgaaatcgatcagtgcttcaggccataaattcggtctggctccgctgggctgcggctgggttatctggcgtgacgaagaagcgctgccgcaggaactggtgttcaacgttgactacctgggtggtcaaattggtacttttgccatcaacttctcccgcccggcgggtcaggtaattgcacagtactatgagttcctgcgcctcggtcgtgaaggctataccaaagtacagaacgcctcttaccaggttgccgcttatctggcggatgaaatcgccaaactggggccgtatgagttcatctgtacgggtcgcccggacgaaggcatcccggcggtttgcttcaaactgaaagatggtgaagatccgggatacaccctgtacgacctctctgaacgtctgcgtctgcgcggctggcaggttccggccttcactctcggcggtgaagccaccgacatcgtggtgatgcgcattatgtgtcgtcgcggcttcgaaatggactttgctgaactgttgctggaagactacaaagcctccctgaaatatctcagcgatcacccgaaactgcaaggtattgcccagcagaacagctttaaacacacctga BBa_K1675003_sequence 1 atggaccagaagctgttaacggatttccgctcagaactactcgattcacgttttggcgcaaaggccatttctactatcgcggagtcaaaacgatttccgctgcacgaaatgcgcgatgatgtcgcatttcagattatcaatgatgaattatatcttgatggcaacgctcgtcagaacctggccactttctgccagacctgggacgacgaaaacgtccataaattgatggatttgtcgatcaataaaaactggatcgacaaagaagaatatccgcaatccgcagccatcgacctgcgttgcgtaaatatggttgccgatctgtggcatgcgcctgcgccgaaaaatggtcaggccgttggcaccaacaccattggttcttccgaggcctgtatgctcggcgggatggcgatgaaatggcgttggcgcaagcgtatggaagctgcgggcaaaccaacggataaaccaaacctggtgtgcggtccggtacaaatctgctggcataaattcgcccgctactgggatgtggagctgcgtgagatccctatgcgccccggtcagttgtttatggacccgaaacgcatgattgaagcctgtgacgaaaacaccatcggcgtggtgccgactttcggcgtgacctacaccggtaactatgagttcccacaaccgctgcacgatgcgctggataaattccaggccgacaccggtatcgacatcgacatgcacatcgacgctgccagcggtggcttcctggcaccgttcgtcgccccggatatcgtctgggacttccgcctgccgcgtgtgaaatcgatcagtgcttcaggccataaattcggtctggctccgctgggctgcggctgggttatctggcgtgacgaagaagcgctgccgcaggaactggtgttcaacgttgactacctgggtggtcaaattggtacttttgccatcaacttctcccgcccggcgggtcaggtaattgcacagtactatgagttcctgcgcctcggtcgtgaaggctataccaaagtacagaacgcctcttaccaggttgccgcttatctggcggatgaaatcgccaaactggggccgtatgagttcatctgtacgggtcgcccggacgaaggcatcccggcggtttgcttcaaactgaaagatggtgaagatccgggatacaccctgtacgacctctctgaacgtctgcgtctgcgcggctggcaggttccggccttcactctcggcggtgaagccaccgacatcgtggtgatgcgcattatgtgtcgtcgcggcttcgaaatggactttgctgaactgttgctggaagactacaaagcctccctgaaatatctcagcgatcacccgaaactgcaaggtattgcccagcagaacagctttaaacacacctga BBa_B0034_sequence 1 aaagaggagaaa BBa_K1170000_sequence 1 cgatcaagactactattattggtagctaaatttcccttaagtcacaatacgttattatcaacgctgtaatttattcagcgtttgtacatatcgttacacgctgaaaccaaccactcacggaagtctgccattcccagggatatagttatttcaacggccccgcagtggggttaaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z