BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1675015
1
BBa_K1675015
J23119+B0034+NhaA+J23119+B0034+NhaB
2015-09-11T11:00:00Z
2015-09-12T02:10:42Z
The gene NhaA and NhaB come from Escherichia coli str. K-12. The J23119 promoter and the B0034 RBS come from the standard parts.
Construct the JBA (J23119+B0034), B0034 and nhaB with the vector pSB1C3. Two functional genes nhaA and nhaB are used to pump H+ in and lower the pH of bacteria???s microenvironment. JBA and JBB are linked with pSB1C3 through 3A Assemble successfully. Thus, it can be an alkaline-resisting part.
false
false
_2093_
27740
27740
9
false
mutation of CTG to CTA Leu.(site:1552bp); mutation of GCT to GCG Ala.(site:1630bp); mutation of ACC to ACT Thr.(site:1780bp); mutation of GAC to GAT Asp.(site:1786bp); mutation of ACC to ACT Thr.(site:1891bp); mutation of GTA to GTG Val.(site:1906bp); mutation of GCA to GCG Ala.(site:2254bp); mutation of GAG to GAA Glu.(site:2281bp); mutation of GTC to GTA Val.(site:2713bp)
false
JIAQI XU
component2452936
1
BBa_B0034
component2452934
1
BBa_J23119
component2452933
1
BBa_K1675000
component2452949
1
BBa_K1675001
component2452927
1
BBa_J23119
component2452929
1
BBa_B0034
annotation2452949
1
BBa_K1675001
range2452949
1
1300
2841
annotation2452934
1
BBa_J23119
range2452934
1
1239
1273
annotation2452933
1
BBa_K1675000
range2452933
1
64
1230
annotation2452929
1
BBa_B0034
range2452929
1
44
55
annotation2452927
1
BBa_J23119
range2452927
1
1
35
annotation2452936
1
BBa_B0034
range2452936
1
1282
1293
BBa_K1675001
1
BBa_K1675001
NhaB; sodium-proton antiporter; membrane protein
2015-09-08T11:00:00Z
2015-09-15T09:57:31Z
Escherichia coli str. K-12 substr. Sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=fasta&from=1233176&to=1234717&strand=true
The gene, NhaB, is the archetypal sodium-proton antiporter in E. coli. It is crucial when the level of NhaA activity is growth limiting, when NhaA is not sufficiently induced, or when NhaA is not activated.
NhaA catalyzes the exchange of 3 proton ion per 2 sodium ion. Therefore, NhaA could make sure E. coli???s survival under alkaline pH, which are in desperate need in our project.
false
false
_2093_
27740
27740
9
false
mutation of CTG to CTA Leu.(site:336bp); mutation of GCT to GCG Ala.(site:414bp); mutation of ACC to ACT Thr.(site:519bp); mutation of GAC to GAT Asp.(site:525bp); mutation of ACC to ACT Thr.(site:630bp); mutation of GTA to GTG Val.(site:645bp); mutation of GCA to GCG Ala.(site:993bp); mutation of GAG to GAA Glu.(site:1020bp); mutation of GTC to GTA Val.(site:1452bp)
false
JIAQI XU
annotation2456966
1
ATG start codon
range2456966
1
1
3
annotation2456965
1
NhaB Coding Region
range2456965
1
1
1542
annotation2456967
1
TGA stop codon
range2456967
1
1540
1542
BBa_K1675000
1
BBa_K1675000
NhaA; sodion-proton antiporter; membrane protein
2015-09-08T11:00:00Z
2015-09-18T11:49:20Z
Escherichia coli str. K-12 substr. Sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=17489&to=18655
The gene,NhaA, is the archetypal sodium-proton antiporter in E. coli. It is the only member of the family that is absolutely required by E. coli for survival in high-salt conditions, under alkaline stress, or in the presence of otherwise toxic Li+ concentration.
NhaA catalyzes the exchange of 2 H+ per Na+. Therefore, nhaA could make sure E. coli???s survival under alkaline pH, which are in desperate need in our project.
false
false
_2093_
27740
27740
9
false
None.
false
JIAQI XU
annotation2447756
1
GTG Start Codon
range2447756
1
1
3
annotation2447757
1
NhaA Coding Region
range2447757
1
1
1167
annotation2447758
1
TGA Stop Codon
range2447758
1
1165
1167
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_K1675015_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagaggtgaaacatctgcatcgattctttagcagtgatgcctcgggaggcattattcttatcattgccgctatcctggcgatgattatggccaacagcggcgcaaccagtggatggtatcacgactttctggagacgccggttcagctccgggttggttcactcgaaatcaacaaaaacatgctgttatggataaatgacgcgctgatggcggtatttttcctgttagtcggtctggaagttaaacgtgaactgatgcaaggatcgctagccagcttacgccaggccgcatttccagttatcgccgctattggtgggatgattgtgccggcattactctatctggcttttaactatgccgatccgattacccgcgaagggtgggcgatcccggcggctactgacattgcttttgcacttggtgtactggcgctgttgggaagtcgtgttccgttagcgctgaagatctttttgatggctctggctattatcgacgatcttggggccatcattatcatcgcattgttctacactaatgacttatcgatggcctctcttggcgtcgcggctgtagcaattgcggtactcgcggtattgaatctgtgtggtgcacgccgcacgggcgtctatattcttgttggcgtggtgttgtggactgcggtgttgaaatcgggggttcacgcaactctggcgggggtaattgtcggcttctttattcctttgaaagagaagcatgggcgttctccagcgaagcgactggagcatgtgttgcacccgtgggtggcgtatctgattttgccgctgtttgcatttgctaatgctggcgtttcactgcaaggcgtcacgctggatggcttgacctccattctgccattggggatcatcgctggcttgctgattggcaaaccgctggggattagtctgttctgctggttggcgctgcgtttgaaactggcgcatctgcctgagggaacgacttatcagcaaattatggtggtggggatcctgtgcggtatcggttttactatgtctatctttattgccagcctggcctttggtagcgtagatccagaactgattaactgggcgaaactcggtatcctggtcggttctatctcttcggcggtaattggatacagctggttacgcgttcgtttgcgtccatcagtttgatactagagttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatggagatctcctggggccgcgcgctatggcgcaattttttgggccagtcccccgactggtacaaactcgccctcatcattttcttaatcgtaaacccgttaattttcctcatcagccctttcgtcgcgggctggttgctggtagcggaatttattttcactctggcgatggccctgaaatgctacccgctgctccccggtggtctgttggctatcgaagcggtattcatcggcatgaccagcgcggaacacgtccgtgaagaggtggcggcaaatcttgaagtcttgctgttactgatgtttatggtggcgggtatctattttatgaaacagctattgctgttcatatttacccgtttgctgttaagcattcgctccaaaatgctgctgtcgctctctttttgcgtggcggcggcgttcctctccgcgttcctcgatgccttaaccgtcgtggcggtggtgatcagcgttgcagtcggtttttatggtatttatcaccgcgtagcctcttcccgtactgaagataccgacctgcaagacgatagtcatatcgacaagcattacaaagtggttctggaacagttccgtggctttctgcgtagcctgatgatgcatgccggtgtcggcactgcattaggcggcgtgatgaccatggtgggcgaaccacagaacctgatcatcgctaaagcggctggctggcattttggcgatttcttcctgcgcatgtcgccggtgaccgttccggttctgatttgtggcctgttaacctgcctgctggtagagaagctgcgttggtttggctacggtgaaacgctgccggagaaagtccgcgaagtgttgcaacagtttgacgatcaaagccgccaccagcgtacccgtcaggataaaatccgtctgattgtccaggcgattattggcgtctggctggtgactgcgctggcgttgcatctggcggaagttggcttgattggtttgtcagtcattattctggcgacatcattgaccggtgtcaccgatgaacatgctatcggtaaagccttcaccgaatctctgccattcaccgcactgttgacggtgtttttctcggtagtcgcggtgattatcgaccaacaactgttttcgccaattattcagtttgtgttgcaggcatcggaacatgctcagctgtcgctgttctatattttcaacggtctgctgtcatccatttcggataacgtcttcgtggggacgatttatatcaacgaagcgaaagcggcaatggaaagtggcgctatcacgttgaagcaatacgagctgctggcggtcgccattaataccggtaccaatctgccctccgtcgctacgccgaacggtcaggctgcgttcctgttcctgctgacctctgcactcgcgccattgattcgcctctcttatggccgcatggtgtggatggccctgccttacaccctcgtactgacactcgtcggcttgctctgcgtcgagtttacgcttgcccctgtaaccgaatggtttatgcaaatgggctggatagcaacgctttga
BBa_K1675001_sequence
1
atggagatctcctggggccgcgcgctatggcgcaattttttgggccagtcccccgactggtacaaactcgccctcatcattttcttaatcgtaaacccgttaattttcctcatcagccctttcgtcgcgggctggttgctggtagcggaatttattttcactctggcgatggccctgaaatgctacccgctgctccccggtggtctgttggctatcgaagcggtattcatcggcatgaccagcgcggaacacgtccgtgaagaggtggcggcaaatcttgaagtcttgctgttactgatgtttatggtggcgggtatctattttatgaaacagctattgctgttcatatttacccgtttgctgttaagcattcgctccaaaatgctgctgtcgctctctttttgcgtggcggcggcgttcctctccgcgttcctcgatgccttaaccgtcgtggcggtggtgatcagcgttgcagtcggtttttatggtatttatcaccgcgtagcctcttcccgtactgaagataccgacctgcaagacgatagtcatatcgacaagcattacaaagtggttctggaacagttccgtggctttctgcgtagcctgatgatgcatgccggtgtcggcactgcattaggcggcgtgatgaccatggtgggcgaaccacagaacctgatcatcgctaaagcggctggctggcattttggcgatttcttcctgcgcatgtcgccggtgaccgttccggttctgatttgtggcctgttaacctgcctgctggtagagaagctgcgttggtttggctacggtgaaacgctgccggagaaagtccgcgaagtgttgcaacagtttgacgatcaaagccgccaccagcgtacccgtcaggataaaatccgtctgattgtccaggcgattattggcgtctggctggtgactgcgctggcgttgcatctggcggaagttggcttgattggtttgtcagtcattattctggcgacatcattgaccggtgtcaccgatgaacatgctatcggtaaagccttcaccgaatctctgccattcaccgcactgttgacggtgtttttctcggtagtcgcggtgattatcgaccaacaactgttttcgccaattattcagtttgtgttgcaggcatcggaacatgctcagctgtcgctgttctatattttcaacggtctgctgtcatccatttcggataacgtcttcgtggggacgatttatatcaacgaagcgaaagcggcaatggaaagtggcgctatcacgttgaagcaatacgagctgctggcggtcgccattaataccggtaccaatctgccctccgtcgctacgccgaacggtcaggctgcgttcctgttcctgctgacctctgcactcgcgccattgattcgcctctcttatggccgcatggtgtggatggccctgccttacaccctcgtactgacactcgtcggcttgctctgcgtcgagtttacgcttgcccctgtaaccgaatggtttatgcaaatgggctggatagcaacgctttga
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1675000_sequence
1
gtgaaacatctgcatcgattctttagcagtgatgcctcgggaggcattattcttatcattgccgctatcctggcgatgattatggccaacagcggcgcaaccagtggatggtatcacgactttctggagacgccggttcagctccgggttggttcactcgaaatcaacaaaaacatgctgttatggataaatgacgcgctgatggcggtatttttcctgttagtcggtctggaagttaaacgtgaactgatgcaaggatcgctagccagcttacgccaggccgcatttccagttatcgccgctattggtgggatgattgtgccggcattactctatctggcttttaactatgccgatccgattacccgcgaagggtgggcgatcccggcggctactgacattgcttttgcacttggtgtactggcgctgttgggaagtcgtgttccgttagcgctgaagatctttttgatggctctggctattatcgacgatcttggggccatcattatcatcgcattgttctacactaatgacttatcgatggcctctcttggcgtcgcggctgtagcaattgcggtactcgcggtattgaatctgtgtggtgcacgccgcacgggcgtctatattcttgttggcgtggtgttgtggactgcggtgttgaaatcgggggttcacgcaactctggcgggggtaattgtcggcttctttattcctttgaaagagaagcatgggcgttctccagcgaagcgactggagcatgtgttgcacccgtgggtggcgtatctgattttgccgctgtttgcatttgctaatgctggcgtttcactgcaaggcgtcacgctggatggcttgacctccattctgccattggggatcatcgctggcttgctgattggcaaaccgctggggattagtctgttctgctggttggcgctgcgtttgaaactggcgcatctgcctgagggaacgacttatcagcaaattatggtggtggggatcctgtgcggtatcggttttactatgtctatctttattgccagcctggcctttggtagcgtagatccagaactgattaactgggcgaaactcggtatcctggtcggttctatctcttcggcggtaattggatacagctggttacgcgttcgtttgcgtccatcagtttga
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z