LOCUS       BBa_K1676049             938 bp    DNA     linear       23-Dec-2024
DEFINITION  GFP Reporter with Mutant RBS 5AC
ACCESSION   BBa_K1676049
VERSION     BBa_K1676049.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..75
                     /label=BBa_K1676013
     CDS             82..801
                     /label=GFP protein
     CDS             82..801
                     /label=BBa_E0040
     stem_loop       821..864
                     /label=stem_loop
     misc_feature    810..889
                     /label=BBa_B0010
     terminator      810..889
                     /label=BBa_B0010
     stem_loop       905..924
                     /label=T7 TE
     misc_feature    931..931
                     /label=stop
     polyA_site      925..938
                     /label=polya
     misc_feature    898..938
                     /label=BBa_B0012
     terminator      898..938
                     /label=BBa_B0012
     terminator      810..938
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagcgg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
      121 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      181 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      241 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      301 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      361 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      421 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      481 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      541 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      601 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      661 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      721 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      781 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
      841 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      901 cactggctca ccttcgggtg ggcctttctg cgtttata
//