LOCUS BBa_K1676053 938 bp DNA linear 29-Nov-2024 DEFINITION GFP Reporter with Mutant RBS 6GC ACCESSION BBa_K1676053 VERSION BBa_K1676053.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 RBS 64..75 /label=BBa_K1676017 CDS 82..801 /label=GFP protein CDS 82..801 /label=BBa_E0040 stem_loop 821..864 /label=stem_loop misc_feature 810..889 /label=BBa_B0010 terminator 810..889 /label=BBa_B0010 stem_loop 905..924 /label=T7 TE misc_feature 931..931 /label=stop polyA_site 925..938 /label=polya misc_feature 898..938 /label=BBa_B0012 terminator 898..938 /label=BBa_B0012 terminator 810..938 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagacg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca 121 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt 181 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta 241 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga 301 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta 361 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag 421 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat 481 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg 541 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat 601 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc 661 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa 721 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg 781 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc 841 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 901 cactggctca ccttcgggtg ggcctttctg cgtttata //