BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1676081
1
BBa_K1676081
Mutant 5 of LDH and wildtype LDP
2015-09-17T11:00:00Z
2015-09-25T02:14:06Z
Wild type LDH and LDP was obtained from a Pseudomonas Strain and random mutagenesis was carried out by PCR to make lots of Mutant LDH. PCR fragments carrying the mutants were then ligated to the wild type LDP to make a complete operon.
This construct is an operon for expression of our mutant LDH and wildtype LDP.
false
false
_2094_
25361
25361
9
false
Primers were designed to have the start and stop codon so that they will not be mutated during PCR
false
Ooi Kean Hean
component2477261
1
BBa_K1676108
component2477254
1
BBa_R0011
component2477260
1
BBa_B0034
component2477264
1
BBa_K1676076
component2477263
1
BBa_B0034
component2477271
1
BBa_B0015
annotation2477254
1
BBa_R0011
range2477254
1
1
54
annotation2477264
1
BBa_K1676076
range2477264
1
1254
2942
annotation2477261
1
BBa_K1676108
range2477261
1
82
1227
annotation2477263
1
BBa_B0034
range2477263
1
1236
1247
annotation2477271
1
BBa_B0015
range2477271
1
2951
3079
annotation2477260
1
BBa_B0034
range2477260
1
64
75
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation2001
1
lac O1
range2001
1
26
42
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation1999
1
lac O1
range1999
1
3
19
annotation2000
1
-35
range2000
1
20
25
annotation2002
1
-10
range2002
1
43
48
BBa_K1676076
1
BBa_K1676076
Lactate Permease
2015-09-17T11:00:00Z
2015-09-18T03:37:23Z
-
Lactate Permease from Pseudomonas. Function in our project is to transport L-lactate into the Shewanella Oneidensis MR1.
false
false
_2094_
25361
25361
9
false
-
false
Ooi Kean Hean
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K1676108
1
BBa_K1676108
Mutant 5 of Lactate Dehydrogenase
2015-09-17T11:00:00Z
2015-09-18T10:16:37Z
Random mutagenesis via error prone PCR was carried out on the gene.
The is a mutant of lactate dehydrogenase which contain mutation to these positions: R310S
false
false
_2094_
25361
25361
9
false
Primers were designed to contain the start and the stop codons so that no mutations would occur on them.
false
Ooi Kean Hean
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1676108_sequence
1
atgatcatctctgcttctactgattaccgtgctgctgctcaacgtaaattaccaccattcttattccactacatcgatggtggtgcttacgctgaatacactttacgtcgtaacgttgaagatttatctgctatcgctttacgtcaacgtgttttaaaaaacatgtctgaattatctttagaaactcgtttattcgatgaaactttagctatgccagttgctttagctccagttggtttaactggtatgtacgctcgtcgtggtgaagttcaagctgctcgtgctgctgctgctaaaggtgttccattcactttatctactgtttctgtttgtccaatcgaagaagttgctccagctatcgatcgtccaatgtggttccaattatacgttttaaaagatcgtggtttcatgcgtaacgctttagaacgtgctaaagctgctggtgttactactttagttttcactgttgatatgccagttccaggtgctcgttaccgtgatgctcactctggtatgtctggtccatacgctgctccacgtcgtatcttacaagctatgactcacccagcttgggcttgggatgttggtttattaggcaagccacacgatctcggtaacatctcggcttaccgtggtaacccaactggtttagaagattacatcggttggttaggtgctaacttcgatccatctatctcttggaaagatttagaatggatccgtgagttttgggatggtccaatggttatcaaaggtatcttagatccagaagatgctcgtgatgctgttaaattcggtgctgatggcatagttgtaagcaaccacggtggtcgtcaattagatggtgttttatcttctgctcgtgctttaccagctatcgctgatgctgttaaaggtgaattagctatcttagctgattctggtatcagtactggtttagatgttgttcgtatgatcgctttaggtgctgattctgttttattaggtcgtgctttcgtttacgctttagctgctgctggtgaagctggtgttcgtaacttattagaattaatcgaaaaagaaatgcgtgttgctatggttttaactggtgctaaatctatcggtgaaatctctgctgattctttagttcgtgaattaggtgcttaa
BBa_K1676081_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgatcatctctgcttctactgattaccgtgctgctgctcaacgtaaattaccaccattcttattccactacatcgatggtggtgcttacgctgaatacactttacgtcgtaacgttgaagatttatctgctatcgctttacgtcaacgtgttttaaaaaacatgtctgaattatctttagaaactcgtttattcgatgaaactttagctatgccagttgctttagctccagttggtttaactggtatgtacgctcgtcgtggtgaagttcaagctgctcgtgctgctgctgctaaaggtgttccattcactttatctactgtttctgtttgtccaatcgaagaagttgctccagctatcgatcgtccaatgtggttccaattatacgttttaaaagatcgtggtttcatgcgtaacgctttagaacgtgctaaagctgctggtgttactactttagttttcactgttgatatgccagttccaggtgctcgttaccgtgatgctcactctggtatgtctggtccatacgctgctccacgtcgtatcttacaagctatgactcacccagcttgggcttgggatgttggtttattaggcaagccacacgatctcggtaacatctcggcttaccgtggtaacccaactggtttagaagattacatcggttggttaggtgctaacttcgatccatctatctcttggaaagatttagaatggatccgtgagttttgggatggtccaatggttatcaaaggtatcttagatccagaagatgctcgtgatgctgttaaattcggtgctgatggcatagttgtaagcaaccacggtggtcgtcaattagatggtgttttatcttctgctcgtgctttaccagctatcgctgatgctgttaaaggtgaattagctatcttagctgattctggtatcagtactggtttagatgttgttcgtatgatcgctttaggtgctgattctgttttattaggtcgtgctttcgtttacgctttagctgctgctggtgaagctggtgttcgtaacttattagaattaatcgaaaaagaaatgcgtgttgctatggttttaactggtgctaaatctatcggtgaaatctctgctgattctttagttcgtgaattaggtgcttaatactagagaaagaggagaaatactagatgcaagcttggcaacaattatacactccattaggttctttaggtttatctgctgctgctgctgttatcccaatcgttttcttcttcttagctttagctgttttccgtttaaaaggtcacatcgctggttctatcactttagctttagctatcgctgttgctatcttcgctttccacatgcccgctgacatggcgttcgctgctgctggctacggtttcttatacggtttatggccaatcgcttggatcatcgttgctgctgttttcttatacaaattaactgttaaatctggtcaattcgaagttatccgttcttctgttttatctatcactggtgatcaacgtttacaagttttattaatcggtttctctttcggtgctttcttagaaggggcggctggcttcggtgctccagttgctatcactgctgctttattagttggtttaggtttcaacccattatacgctgctggtttatgtttaatcgctaacactgctccagttgctttcggtgctttaggtatcccaatcatcgttgctggtcaagttactggcatagatgcgttcaaaatcggtgcgatgactggtcgtcaattaccattcttatctatcttagttccattctggttagttttcatgatggatggttggcgtggtatccgtgaaacttggccagctgctatcgttgctggtggttctttcgctatcactcaatacttaacttctaacttcatcggtccagaattaccagatatcacttctgctttagtttctttagtttctttaactttattcttaaaagtttggcaaccacaaggtgcgtcggaagctctcgctgctggtggcggtgctgctgttgctgttgctggtggtggtttcggtggtcaacgttctgttgaaccatctgcttactctttcggtgaaatcttaaaagcttggtctccattcttagttttaactgttttagttactatctggactttaaaaccattcaaagctttattcgctccaggtggtgaattatactggttagttttcaacttcgctatcccacacttagatcaattagttgttaaaggtgctccaatcgctacttctgctactccaatcgctgctgttttcaaattcgatccaatctctgctactggtactgctatcttcttatctgctttagtttctatgttcatcttacgtatcggtgctaaaatcggtatcactacttttaaggaagtgctggttgaattaaaatggccaatcttatctatcggtatggttttagctttcgctttcgttactaactactctggtatgtctactactttagctttagttttagctggtactggtgctgctttcccattcttctctccattcttaggttggttaggtgttttcttaactggttctgatacttcttctaacgctttattctcttctttacaatctactactgctcatcaaataggtgtaagcgatactttattagttgctgctaacacttctggtggtgttactggtaaaatgatctctccacaatctatcgctgttgcttgtgctgctactggtatggttggtaaagaatctgatttattccgtttcactttaaaacactctttaatcttcgctgctttagttggtttaatcactttagctcaagcttacttattcactggtatgttagttcactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1676076_sequence
1
atgcaagcttggcaacaattatacactccattaggttctttaggtttatctgctgctgctgctgttatcccaatcgttttcttcttcttagctttagctgttttccgtttaaaaggtcacatcgctggttctatcactttagctttagctatcgctgttgctatcttcgctttccacatgcccgctgacatggcgttcgctgctgctggctacggtttcttatacggtttatggccaatcgcttggatcatcgttgctgctgttttcttatacaaattaactgttaaatctggtcaattcgaagttatccgttcttctgttttatctatcactggtgatcaacgtttacaagttttattaatcggtttctctttcggtgctttcttagaaggggcggctggcttcggtgctccagttgctatcactgctgctttattagttggtttaggtttcaacccattatacgctgctggtttatgtttaatcgctaacactgctccagttgctttcggtgctttaggtatcccaatcatcgttgctggtcaagttactggcatagatgcgttcaaaatcggtgcgatgactggtcgtcaattaccattcttatctatcttagttccattctggttagttttcatgatggatggttggcgtggtatccgtgaaacttggccagctgctatcgttgctggtggttctttcgctatcactcaatacttaacttctaacttcatcggtccagaattaccagatatcacttctgctttagtttctttagtttctttaactttattcttaaaagtttggcaaccacaaggtgcgtcggaagctctcgctgctggtggcggtgctgctgttgctgttgctggtggtggtttcggtggtcaacgttctgttgaaccatctgcttactctttcggtgaaatcttaaaagcttggtctccattcttagttttaactgttttagttactatctggactttaaaaccattcaaagctttattcgctccaggtggtgaattatactggttagttttcaacttcgctatcccacacttagatcaattagttgttaaaggtgctccaatcgctacttctgctactccaatcgctgctgttttcaaattcgatccaatctctgctactggtactgctatcttcttatctgctttagtttctatgttcatcttacgtatcggtgctaaaatcggtatcactacttttaaggaagtgctggttgaattaaaatggccaatcttatctatcggtatggttttagctttcgctttcgttactaactactctggtatgtctactactttagctttagttttagctggtactggtgctgctttcccattcttctctccattcttaggttggttaggtgttttcttaactggttctgatacttcttctaacgctttattctcttctttacaatctactactgctcatcaaataggtgtaagcgatactttattagttgctgctaacacttctggtggtgttactggtaaaatgatctctccacaatctatcgctgttgcttgtgctgctactggtatggttggtaaagaatctgatttattccgtttcactttaaaacactctttaatcttcgctgctttagttggtttaatcactttagctcaagcttacttattcactggtatgttagttcactaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z