BBa_K1676089 1 BBa_K1676089 Mutant 13 of LDH and wildtype LDP 2015-09-17T11:00:00Z 2015-09-25T08:24:22Z Wild type LDH and LDP was obtained from a Pseudomonas Strain and random mutagenesis was carried out by PCR to make lots of Mutant LDH. PCR fragments carrying the mutants were then ligated to the wild type LDP to make a complete operon. This construct is an operon for expression of our mutant LDH and wildtype LDP. false false _2094_ 25361 25361 9 false Primers were designed to have the start and stop codon so that they will not be mutated during PCR false Ooi Kean Hean component2477544 1 BBa_B0015 component2477537 1 BBa_K1676076 component2477536 1 BBa_B0034 component2477533 1 BBa_B0034 component2477527 1 BBa_R0011 component2477534 1 BBa_K1676117 annotation2477534 1 BBa_K1676117 range2477534 1 82 1227 annotation2477536 1 BBa_B0034 range2477536 1 1236 1247 annotation2477544 1 BBa_B0015 range2477544 1 2951 3079 annotation2477533 1 BBa_B0034 range2477533 1 64 75 annotation2477537 1 BBa_K1676076 range2477537 1 1254 2942 annotation2477527 1 BBa_R0011 range2477527 1 1 54 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1676117 1 BBa_K1676117 Mutant 13 of Lactate Dehydrogenase 2015-09-24T11:00:00Z 2015-09-25T01:46:08Z Random mutagenesis via error prone PCR was carried out on the gene The is a mutant of lactate dehydrogenase which contain mutation to these positions: false false _2094_ 25361 25361 9 false Primers were designed to contain the start and the stop codons so that no mutations would occur on them false Ooi Kean Hean BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2002 1 -10 range2002 1 43 48 annotation1999 1 lac O1 range1999 1 3 19 annotation2000 1 -35 range2000 1 20 25 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation2001 1 lac O1 range2001 1 26 42 BBa_K1676076 1 BBa_K1676076 Lactate Permease 2015-09-17T11:00:00Z 2015-09-18T03:37:23Z - Lactate Permease from Pseudomonas. Function in our project is to transport L-lactate into the Shewanella Oneidensis MR1. false false _2094_ 25361 25361 9 false - false Ooi Kean Hean BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K1676117_sequence 1 atgatcatctctgcttctactgattaccgtgctgctgctcaacgtaaattaccatcattcttattccactacatcgatggtggtgcttacgatgaatacactttatgtcgtaacgttgaagatttatctgctatcgctttacgtcaacgtgttttaaaaaacatgtctgaattatctttagaaactcgtatattcgatgaaactttagctatgccagttgctttagctccagttggtttaactggtatgtacgctcgtcgtggtgaagttcaagctgctcgtgctgctgctgctaaaggtgttccattcactttatctactgtttctgtttgtccaatcgaagaagttgctccagctatcgatcgtccaatgtggttccaatcatacgttttaaaagatcgtggtttcatgcgtaacgctttagaacgtgctaaaggtgctggtgttactactttagttttcactgttgatatgccagttccaggtgctcgttaccgtgacgctcactctggtatgtctggtccatacgctgctccacgtcgtatcttacaagctatgactcacccagcttgggcttgggatgttggtttattaggcaagccacacgatctcggtaacatctcggcttaccgtggtaacccaactggtttagaagattacatcggttggttaggtgctaacttcgatccatctatctcttggaaagatttagaatggatccgtgagttttgggatggtccaatggttattaaaggtatcttagatccagaagatgctcgtgatgctgttaaattcggtgctgatggcatagttgtaagcaaccacggtggtcgtcaattagatggtgttttatcttctgctcgtgctttaccatctatcgctgatgctgttaaaggtgaattagctatcttagctgattctggtatccgtacaggtttagatgttgttcgtatgatcgctctaggtgctgattctgttttattaggtcgtgctttcatttacgctttagctgctgctggtgaagctggtgttcgtaacttattagaattaatcgaaaaagaaatgcgtgttgctatagttttaactggtgctaaatctatgggtgaaatctctgctgattctttagttcgtgaattagatgcttaa BBa_K1676089_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgatcatctctgcttctactgattaccgtgctgctgctcaacgtaaattaccatcattcttattccactacatcgatggtggtgcttacgatgaatacactttatgtcgtaacgttgaagatttatctgctatcgctttacgtcaacgtgttttaaaaaacatgtctgaattatctttagaaactcgtatattcgatgaaactttagctatgccagttgctttagctccagttggtttaactggtatgtacgctcgtcgtggtgaagttcaagctgctcgtgctgctgctgctaaaggtgttccattcactttatctactgtttctgtttgtccaatcgaagaagttgctccagctatcgatcgtccaatgtggttccaatcatacgttttaaaagatcgtggtttcatgcgtaacgctttagaacgtgctaaaggtgctggtgttactactttagttttcactgttgatatgccagttccaggtgctcgttaccgtgacgctcactctggtatgtctggtccatacgctgctccacgtcgtatcttacaagctatgactcacccagcttgggcttgggatgttggtttattaggcaagccacacgatctcggtaacatctcggcttaccgtggtaacccaactggtttagaagattacatcggttggttaggtgctaacttcgatccatctatctcttggaaagatttagaatggatccgtgagttttgggatggtccaatggttattaaaggtatcttagatccagaagatgctcgtgatgctgttaaattcggtgctgatggcatagttgtaagcaaccacggtggtcgtcaattagatggtgttttatcttctgctcgtgctttaccatctatcgctgatgctgttaaaggtgaattagctatcttagctgattctggtatccgtacaggtttagatgttgttcgtatgatcgctctaggtgctgattctgttttattaggtcgtgctttcatttacgctttagctgctgctggtgaagctggtgttcgtaacttattagaattaatcgaaaaagaaatgcgtgttgctatagttttaactggtgctaaatctatgggtgaaatctctgctgattctttagttcgtgaattagatgcttaatactagagaaagaggagaaatactagatgcaagcttggcaacaattatacactccattaggttctttaggtttatctgctgctgctgctgttatcccaatcgttttcttcttcttagctttagctgttttccgtttaaaaggtcacatcgctggttctatcactttagctttagctatcgctgttgctatcttcgctttccacatgcccgctgacatggcgttcgctgctgctggctacggtttcttatacggtttatggccaatcgcttggatcatcgttgctgctgttttcttatacaaattaactgttaaatctggtcaattcgaagttatccgttcttctgttttatctatcactggtgatcaacgtttacaagttttattaatcggtttctctttcggtgctttcttagaaggggcggctggcttcggtgctccagttgctatcactgctgctttattagttggtttaggtttcaacccattatacgctgctggtttatgtttaatcgctaacactgctccagttgctttcggtgctttaggtatcccaatcatcgttgctggtcaagttactggcatagatgcgttcaaaatcggtgcgatgactggtcgtcaattaccattcttatctatcttagttccattctggttagttttcatgatggatggttggcgtggtatccgtgaaacttggccagctgctatcgttgctggtggttctttcgctatcactcaatacttaacttctaacttcatcggtccagaattaccagatatcacttctgctttagtttctttagtttctttaactttattcttaaaagtttggcaaccacaaggtgcgtcggaagctctcgctgctggtggcggtgctgctgttgctgttgctggtggtggtttcggtggtcaacgttctgttgaaccatctgcttactctttcggtgaaatcttaaaagcttggtctccattcttagttttaactgttttagttactatctggactttaaaaccattcaaagctttattcgctccaggtggtgaattatactggttagttttcaacttcgctatcccacacttagatcaattagttgttaaaggtgctccaatcgctacttctgctactccaatcgctgctgttttcaaattcgatccaatctctgctactggtactgctatcttcttatctgctttagtttctatgttcatcttacgtatcggtgctaaaatcggtatcactacttttaaggaagtgctggttgaattaaaatggccaatcttatctatcggtatggttttagctttcgctttcgttactaactactctggtatgtctactactttagctttagttttagctggtactggtgctgctttcccattcttctctccattcttaggttggttaggtgttttcttaactggttctgatacttcttctaacgctttattctcttctttacaatctactactgctcatcaaataggtgtaagcgatactttattagttgctgctaacacttctggtggtgttactggtaaaatgatctctccacaatctatcgctgttgcttgtgctgctactggtatggttggtaaagaatctgatttattccgtttcactttaaaacactctttaatcttcgctgctttagttggtttaatcactttagctcaagcttacttattcactggtatgttagttcactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1676076_sequence 1 atgcaagcttggcaacaattatacactccattaggttctttaggtttatctgctgctgctgctgttatcccaatcgttttcttcttcttagctttagctgttttccgtttaaaaggtcacatcgctggttctatcactttagctttagctatcgctgttgctatcttcgctttccacatgcccgctgacatggcgttcgctgctgctggctacggtttcttatacggtttatggccaatcgcttggatcatcgttgctgctgttttcttatacaaattaactgttaaatctggtcaattcgaagttatccgttcttctgttttatctatcactggtgatcaacgtttacaagttttattaatcggtttctctttcggtgctttcttagaaggggcggctggcttcggtgctccagttgctatcactgctgctttattagttggtttaggtttcaacccattatacgctgctggtttatgtttaatcgctaacactgctccagttgctttcggtgctttaggtatcccaatcatcgttgctggtcaagttactggcatagatgcgttcaaaatcggtgcgatgactggtcgtcaattaccattcttatctatcttagttccattctggttagttttcatgatggatggttggcgtggtatccgtgaaacttggccagctgctatcgttgctggtggttctttcgctatcactcaatacttaacttctaacttcatcggtccagaattaccagatatcacttctgctttagtttctttagtttctttaactttattcttaaaagtttggcaaccacaaggtgcgtcggaagctctcgctgctggtggcggtgctgctgttgctgttgctggtggtggtttcggtggtcaacgttctgttgaaccatctgcttactctttcggtgaaatcttaaaagcttggtctccattcttagttttaactgttttagttactatctggactttaaaaccattcaaagctttattcgctccaggtggtgaattatactggttagttttcaacttcgctatcccacacttagatcaattagttgttaaaggtgctccaatcgctacttctgctactccaatcgctgctgttttcaaattcgatccaatctctgctactggtactgctatcttcttatctgctttagtttctatgttcatcttacgtatcggtgctaaaatcggtatcactacttttaaggaagtgctggttgaattaaaatggccaatcttatctatcggtatggttttagctttcgctttcgttactaactactctggtatgtctactactttagctttagttttagctggtactggtgctgctttcccattcttctctccattcttaggttggttaggtgttttcttaactggttctgatacttcttctaacgctttattctcttctttacaatctactactgctcatcaaataggtgtaagcgatactttattagttgctgctaacacttctggtggtgttactggtaaaatgatctctccacaatctatcgctgttgcttgtgctgctactggtatggttggtaaagaatctgatttattccgtttcactttaaaacactctttaatcttcgctgctttagttggtttaatcactttagctcaagcttacttattcactggtatgttagttcactaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z