LOCUS       BBa_K1676096            1927 bp    DNA     linear       27-Dec-2024
DEFINITION  Mutant 20 of LDH and wildtype LDP
ACCESSION   BBa_K1676096
VERSION     BBa_K1676096.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    88..91
                     /label=conserved
     RBS             84..95
                     /label=BBa_B0034
     CDS             102..1790
                     /label=BBa_K1676076
     stem_loop       1810..1853
                     /label=stem_loop
     misc_feature    1799..1878
                     /label=BBa_B0010
     terminator      1799..1878
                     /label=BBa_B0010
     stem_loop       1894..1913
                     /label=T7 TE
     misc_feature    1920..1920
                     /label=stop
     polyA_site      1914..1927
                     /label=polya
     misc_feature    1887..1927
                     /label=BBa_B0012
     terminator      1887..1927
                     /label=BBa_B0012
     terminator      1799..1927
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gagaaagagg agaaatacta gatgcaagct tggcaacaat
      121 tatacactcc attaggttct ttaggtttat ctgctgctgc tgctgttatc ccaatcgttt
      181 tcttcttctt agctttagct gttttccgtt taaaaggtca catcgctggt tctatcactt
      241 tagctttagc tatcgctgtt gctatcttcg ctttccacat gcccgctgac atggcgttcg
      301 ctgctgctgg ctacggtttc ttatacggtt tatggccaat cgcttggatc atcgttgctg
      361 ctgttttctt atacaaatta actgttaaat ctggtcaatt cgaagttatc cgttcttctg
      421 ttttatctat cactggtgat caacgtttac aagttttatt aatcggtttc tctttcggtg
      481 ctttcttaga aggggcggct ggcttcggtg ctccagttgc tatcactgct gctttattag
      541 ttggtttagg tttcaaccca ttatacgctg ctggtttatg tttaatcgct aacactgctc
      601 cagttgcttt cggtgcttta ggtatcccaa tcatcgttgc tggtcaagtt actggcatag
      661 atgcgttcaa aatcggtgcg atgactggtc gtcaattacc attcttatct atcttagttc
      721 cattctggtt agttttcatg atggatggtt ggcgtggtat ccgtgaaact tggccagctg
      781 ctatcgttgc tggtggttct ttcgctatca ctcaatactt aacttctaac ttcatcggtc
      841 cagaattacc agatatcact tctgctttag tttctttagt ttctttaact ttattcttaa
      901 aagtttggca accacaaggt gcgtcggaag ctctcgctgc tggtggcggt gctgctgttg
      961 ctgttgctgg tggtggtttc ggtggtcaac gttctgttga accatctgct tactctttcg
     1021 gtgaaatctt aaaagcttgg tctccattct tagttttaac tgttttagtt actatctgga
     1081 ctttaaaacc attcaaagct ttattcgctc caggtggtga attatactgg ttagttttca
     1141 acttcgctat cccacactta gatcaattag ttgttaaagg tgctccaatc gctacttctg
     1201 ctactccaat cgctgctgtt ttcaaattcg atccaatctc tgctactggt actgctatct
     1261 tcttatctgc tttagtttct atgttcatct tacgtatcgg tgctaaaatc ggtatcacta
     1321 cttttaagga agtgctggtt gaattaaaat ggccaatctt atctatcggt atggttttag
     1381 ctttcgcttt cgttactaac tactctggta tgtctactac tttagcttta gttttagctg
     1441 gtactggtgc tgctttccca ttcttctctc cattcttagg ttggttaggt gttttcttaa
     1501 ctggttctga tacttcttct aacgctttat tctcttcttt acaatctact actgctcatc
     1561 aaataggtgt aagcgatact ttattagttg ctgctaacac ttctggtggt gttactggta
     1621 aaatgatctc tccacaatct atcgctgttg cttgtgctgc tactggtatg gttggtaaag
     1681 aatctgattt attccgtttc actttaaaac actctttaat cttcgctgct ttagttggtt
     1741 taatcacttt agctcaagct tacttattca ctggtatgtt agttcactaa tactagagcc
     1801 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1861 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1921 gtttata
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