BBa_K1676097 1 BBa_K1676097 Mutant 1 of Lactate Permease 2015-09-17T11:00:00Z 2015-09-18T10:41:25Z Random mutagenesis was carried out via PCR to obtain the mutants. Lactate Permease from Pseudomonas. Function in our project is to transport L-lactate into the Shewanella Oneidensis MR1. This mutant contains the mutation of L105V, F120L, V169A, I190K, D183G false false _2094_ 25361 25361 9 false Primers were designed to have the start and stop codon so that they will not be mutated during PCR false Ooi Kean Hean BBa_K1676097_sequence 1 atgcaagcttggcaacaattatacactccattaggttctttaggtttatctgctgctgctgctgttatcccaatcgttttcttcttcttagctttagctgttttccgtttaaaaggtcacatcgctggttctatcactttagctttagctatcgctgttgctatcttcgctttccacatgcccgctgacatggcgttcgctgctgctggctacggtttcttatacggtttatggccaatcgcttggatcatcgttgctgctgttttcttatacaaattaactgttaaatctggtcaattcgaagttatccgttcttctgttttatctatcactggtgatcaacgtttacaagttttattaatcggtttctctttcggtgctttcttagaaggggcggctggcttcggtgctccagttgctatcactgctgctttattagttggtttaggtttcaacccattatacgctgctggtttatgtttaatcgctaacactgctccagttgctttcggtgctttaggtatcccaatcatcgttgctggtcaagttactggcatagatgcgttcaaaatcggtgcgatgactggtcgtcaattaccattcttatctatcttagttccattctggttagttttcatgatggatggttggcgtggtatccgtgaaacttggccagctgctatcgttgctggtggttctttcgctatcactcaatacttaacttctaacttcatcggtccagaattaccagatatcacttctgctttagtttctttagtttctttaactttattcttaaaagtttggcaaccacaaggtgcgtcggaagctctcgctgctggtggcggtgctgctgttgctgttgctggtggtggtttcggtggtcaacgttctgttgaaccatctgcttactctttcggtgaaatcttaaaagcttggtctccattcttagttttaactgttttagttactatctggactttaaaaccattcaaagctttattcgctccaggtggtgaattatactggttagttttcaacttcgctatcccacacttagatcaattagttgttaaaggtgctccaatcgctacttctgctactccaatcgctgctgttttcaaattcgatccaatctctgctactggtactgctatcttcttatctgctttagtttctatgttcatcttacgtatcggtgctaaaatcggtatcactacttttaaggaagtgctggttgaattaaaatggccaatcttatctatcggtatggttttagctttcgctttcgttactaactactctggtatgtctactactttagctttagttttagctggtactggtgctgctttcccattcttctctccattcttaggttggttaggtgttttcttaactggttctgatacttcttctaacgctttattctcttctttacaatctactactgctcatcaaataggtgtaagcgatactttattagttgctgctaacacttctggtggtgttactggtaaaatgatctctccacaatctatcgctgttgcttgtgctgctactggtatggttggtaaagaatctgatttattccgtttcactttaaaacactctttaatcttcgctgctttagttggtttaatcactttagctcaagcttacttattcactggtatgttagttcactaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z