LOCUS       BBa_K1676098            1907 bp    DNA     linear       09-Dec-2025
DEFINITION  Mutant 1 of Lactate Permease Expression Part
ACCESSION   BBa_K1676098
VERSION     BBa_K1676098.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..1770
                     /label=BBa_K1676097
     stem_loop       1790..1833
                     /label=stem_loop
     misc_feature    1779..1858
                     /label=BBa_B0010
     terminator      1779..1858
                     /label=BBa_B0010
     stem_loop       1874..1893
                     /label=T7 TE
     misc_feature    1900..1900
                     /label=stop
     polyA_site      1894..1907
                     /label=polya
     misc_feature    1867..1907
                     /label=BBa_B0012
     terminator      1867..1907
                     /label=BBa_B0012
     terminator      1779..1907
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgcaagct tggcaacaat tatacactcc attaggttct
      121 ttaggtttat ctgctgctgc tgctgttatc ccaatcgttt tcttcttctt agctttagct
      181 gttttccgtt taaaaggtca catcgctggt tctatcactt tagctttagc tatcgctgtt
      241 gctatcttcg ctttccacat gcccgctgac atggcgttcg ctgctgctgg ctacggtttc
      301 ttatacggtt tatggccaat cgcttggatc atcgttgctg ctgttttctt atacaaatta
      361 actgttaaat ctggtcaatt cgaagttatc cgttcttctg ttttatctat cactggtgat
      421 caacgtttac aagttttatt aatcggtttc tctttcggtg ctttcttaga aggggcggct
      481 ggcttcggtg ctccagttgc tatcactgct gctttattag ttggtttagg tttcaaccca
      541 ttatacgctg ctggtttatg tttaatcgct aacactgctc cagttgcttt cggtgcttta
      601 ggtatcccaa tcatcgttgc tggtcaagtt actggcatag atgcgttcaa aatcggtgcg
      661 atgactggtc gtcaattacc attcttatct atcttagttc cattctggtt agttttcatg
      721 atggatggtt ggcgtggtat ccgtgaaact tggccagctg ctatcgttgc tggtggttct
      781 ttcgctatca ctcaatactt aacttctaac ttcatcggtc cagaattacc agatatcact
      841 tctgctttag tttctttagt ttctttaact ttattcttaa aagtttggca accacaaggt
      901 gcgtcggaag ctctcgctgc tggtggcggt gctgctgttg ctgttgctgg tggtggtttc
      961 ggtggtcaac gttctgttga accatctgct tactctttcg gtgaaatctt aaaagcttgg
     1021 tctccattct tagttttaac tgttttagtt actatctgga ctttaaaacc attcaaagct
     1081 ttattcgctc caggtggtga attatactgg ttagttttca acttcgctat cccacactta
     1141 gatcaattag ttgttaaagg tgctccaatc gctacttctg ctactccaat cgctgctgtt
     1201 ttcaaattcg atccaatctc tgctactggt actgctatct tcttatctgc tttagtttct
     1261 atgttcatct tacgtatcgg tgctaaaatc ggtatcacta cttttaagga agtgctggtt
     1321 gaattaaaat ggccaatctt atctatcggt atggttttag ctttcgcttt cgttactaac
     1381 tactctggta tgtctactac tttagcttta gttttagctg gtactggtgc tgctttccca
     1441 ttcttctctc cattcttagg ttggttaggt gttttcttaa ctggttctga tacttcttct
     1501 aacgctttat tctcttcttt acaatctact actgctcatc aaataggtgt aagcgatact
     1561 ttattagttg ctgctaacac ttctggtggt gttactggta aaatgatctc tccacaatct
     1621 atcgctgttg cttgtgctgc tactggtatg gttggtaaag aatctgattt attccgtttc
     1681 actttaaaac actctttaat cttcgctgct ttagttggtt taatcacttt agctcaagct
     1741 tacttattca ctggtatgtt agttcactaa tactagagcc aggcatcaaa taaaacgaaa
     1801 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1861 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
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