BBa_K1676099 1 BBa_K1676099 Wildtype Lactate Permease and Lactate Dehydrogenase Operon 2015-09-17T11:00:00Z 2015-09-18T10:45:05Z Wild type LDP was obtained from a Pseudomonas Strain and then ligated together to obtain this construct This construct is an operon for expression of wildtype LDH and LDP with pLac as a promoter. However, this in an intermediate part which lacks a transcription terminator. false false _2094_ 25361 25361 9 false - false Ooi Kean Hean component2476058 1 BBa_K1676112 component2476051 1 BBa_R0011 component2476061 1 BBa_K1676097 component2476060 1 BBa_B0034 component2476057 1 BBa_B0034 annotation2476058 1 BBa_K1676112 range2476058 1 82 1227 annotation2476057 1 BBa_B0034 range2476057 1 64 75 annotation2476051 1 BBa_R0011 range2476051 1 1 54 annotation2476061 1 BBa_K1676097 range2476061 1 1254 2942 annotation2476060 1 BBa_B0034 range2476060 1 1236 1247 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1676112 1 BBa_K1676112 Wildtype Lactate Dehydrogenase 2015-09-17T11:00:00Z 2015-09-18T10:33:42Z - This enzyme is from the species Psedomonas aeruginosa, which is found to have high lactate oxidizing activity in Shewanella oneidensis MR1 compared to other species. This enzyme catalyses the oxidation of lactate to pyruvate with NAD+ as a cofactor. false false _2094_ 25361 25361 9 false - false Ooi Kean Hean BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation1999 1 lac O1 range1999 1 3 19 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation2000 1 -35 range2000 1 20 25 annotation2001 1 lac O1 range2001 1 26 42 annotation2002 1 -10 range2002 1 43 48 BBa_K1676097 1 BBa_K1676097 Mutant 1 of Lactate Permease 2015-09-17T11:00:00Z 2015-09-18T10:41:25Z Random mutagenesis was carried out via PCR to obtain the mutants. Lactate Permease from Pseudomonas. Function in our project is to transport L-lactate into the Shewanella Oneidensis MR1. This mutant contains the mutation of L105V, F120L, V169A, I190K, D183G false false _2094_ 25361 25361 9 false Primers were designed to have the start and stop codon so that they will not be mutated during PCR false Ooi Kean Hean BBa_B0034_sequence 1 aaagaggagaaa BBa_K1676112_sequence 1 atgatcatctctgcttctactgattaccgtgctgctgctcaacgtaaattaccaccattcttattccactacatcgatggtggtgcttacgctgaatacactttacgtcgtaacgttgaagatttatctgctatcgctttacgtcaacgtgttttaaaaaacatgtctgaattatctttagaaactcgtttattcgatgaaactttagctatgccagttgctttagctccagttggtttaactggtatgtacgctcgtcgtggtgaagttcaagctgctcgtgctgctgctgctaaaggtgttccattcactttatctactgtttctgtttgtccaatcgaagaagttgctccagctatcgatcgtccaatgtggttccaattatacgttttaaaagatcgtggtttcatgcgtaacgctttagaacgtgctaaagctgctggtgttactactttagttttcactgttgatatgccagttccaggtgctcgttaccgtgatgctcactctggtatgtctggtccatacgctgctccacgtcgtatcttacaagctatgactcacccagcttgggcttgggatgttggtttattaggcaagccacacgatctcggtaacatctcggcttaccgtggtaacccaactggtttagaagattacatcggttggttaggtgctaacttcgatccatctatctcttggaaagatttagaatggatccgtgagttttgggatggtccaatggttatcaaaggtatcttagatccagaagatgctcgtgatgctgttaaattcggtgctgatggcatagttgtaagcaaccacggtggtcgtcaattagatggtgttttatcttctgctcgtgctttaccagctatcgctgatgctgttaaaggtgaattagctatcttagctgattctggtatccgtactggtttagatgttgttcgtatgatcgctttaggtgctgattctgttttattaggtcgtgctttcgtttacgctttagctgctgctggtgaagctggtgttcgtaacttattagaattaatcgaaaaagaaatgcgtgttgctatggttttaactggtgctaaatctatcggtgaaatctctgctgattctttagttcgtgaattaggtgcttaa BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca BBa_K1676099_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgatcatctctgcttctactgattaccgtgctgctgctcaacgtaaattaccaccattcttattccactacatcgatggtggtgcttacgctgaatacactttacgtcgtaacgttgaagatttatctgctatcgctttacgtcaacgtgttttaaaaaacatgtctgaattatctttagaaactcgtttattcgatgaaactttagctatgccagttgctttagctccagttggtttaactggtatgtacgctcgtcgtggtgaagttcaagctgctcgtgctgctgctgctaaaggtgttccattcactttatctactgtttctgtttgtccaatcgaagaagttgctccagctatcgatcgtccaatgtggttccaattatacgttttaaaagatcgtggtttcatgcgtaacgctttagaacgtgctaaagctgctggtgttactactttagttttcactgttgatatgccagttccaggtgctcgttaccgtgatgctcactctggtatgtctggtccatacgctgctccacgtcgtatcttacaagctatgactcacccagcttgggcttgggatgttggtttattaggcaagccacacgatctcggtaacatctcggcttaccgtggtaacccaactggtttagaagattacatcggttggttaggtgctaacttcgatccatctatctcttggaaagatttagaatggatccgtgagttttgggatggtccaatggttatcaaaggtatcttagatccagaagatgctcgtgatgctgttaaattcggtgctgatggcatagttgtaagcaaccacggtggtcgtcaattagatggtgttttatcttctgctcgtgctttaccagctatcgctgatgctgttaaaggtgaattagctatcttagctgattctggtatccgtactggtttagatgttgttcgtatgatcgctttaggtgctgattctgttttattaggtcgtgctttcgtttacgctttagctgctgctggtgaagctggtgttcgtaacttattagaattaatcgaaaaagaaatgcgtgttgctatggttttaactggtgctaaatctatcggtgaaatctctgctgattctttagttcgtgaattaggtgcttaatactagagaaagaggagaaatactagatgcaagcttggcaacaattatacactccattaggttctttaggtttatctgctgctgctgctgttatcccaatcgttttcttcttcttagctttagctgttttccgtttaaaaggtcacatcgctggttctatcactttagctttagctatcgctgttgctatcttcgctttccacatgcccgctgacatggcgttcgctgctgctggctacggtttcttatacggtttatggccaatcgcttggatcatcgttgctgctgttttcttatacaaattaactgttaaatctggtcaattcgaagttatccgttcttctgttttatctatcactggtgatcaacgtttacaagttttattaatcggtttctctttcggtgctttcttagaaggggcggctggcttcggtgctccagttgctatcactgctgctttattagttggtttaggtttcaacccattatacgctgctggtttatgtttaatcgctaacactgctccagttgctttcggtgctttaggtatcccaatcatcgttgctggtcaagttactggcatagatgcgttcaaaatcggtgcgatgactggtcgtcaattaccattcttatctatcttagttccattctggttagttttcatgatggatggttggcgtggtatccgtgaaacttggccagctgctatcgttgctggtggttctttcgctatcactcaatacttaacttctaacttcatcggtccagaattaccagatatcacttctgctttagtttctttagtttctttaactttattcttaaaagtttggcaaccacaaggtgcgtcggaagctctcgctgctggtggcggtgctgctgttgctgttgctggtggtggtttcggtggtcaacgttctgttgaaccatctgcttactctttcggtgaaatcttaaaagcttggtctccattcttagttttaactgttttagttactatctggactttaaaaccattcaaagctttattcgctccaggtggtgaattatactggttagttttcaacttcgctatcccacacttagatcaattagttgttaaaggtgctccaatcgctacttctgctactccaatcgctgctgttttcaaattcgatccaatctctgctactggtactgctatcttcttatctgctttagtttctatgttcatcttacgtatcggtgctaaaatcggtatcactacttttaaggaagtgctggttgaattaaaatggccaatcttatctatcggtatggttttagctttcgctttcgttactaactactctggtatgtctactactttagctttagttttagctggtactggtgctgctttcccattcttctctccattcttaggttggttaggtgttttcttaactggttctgatacttcttctaacgctttattctcttctttacaatctactactgctcatcaaataggtgtaagcgatactttattagttgctgctaacacttctggtggtgttactggtaaaatgatctctccacaatctatcgctgttgcttgtgctgctactggtatggttggtaaagaatctgatttattccgtttcactttaaaacactctttaatcttcgctgctttagttggtttaatcactttagctcaagcttacttattcactggtatgttagttcactaa BBa_K1676097_sequence 1 atgcaagcttggcaacaattatacactccattaggttctttaggtttatctgctgctgctgctgttatcccaatcgttttcttcttcttagctttagctgttttccgtttaaaaggtcacatcgctggttctatcactttagctttagctatcgctgttgctatcttcgctttccacatgcccgctgacatggcgttcgctgctgctggctacggtttcttatacggtttatggccaatcgcttggatcatcgttgctgctgttttcttatacaaattaactgttaaatctggtcaattcgaagttatccgttcttctgttttatctatcactggtgatcaacgtttacaagttttattaatcggtttctctttcggtgctttcttagaaggggcggctggcttcggtgctccagttgctatcactgctgctttattagttggtttaggtttcaacccattatacgctgctggtttatgtttaatcgctaacactgctccagttgctttcggtgctttaggtatcccaatcatcgttgctggtcaagttactggcatagatgcgttcaaaatcggtgcgatgactggtcgtcaattaccattcttatctatcttagttccattctggttagttttcatgatggatggttggcgtggtatccgtgaaacttggccagctgctatcgttgctggtggttctttcgctatcactcaatacttaacttctaacttcatcggtccagaattaccagatatcacttctgctttagtttctttagtttctttaactttattcttaaaagtttggcaaccacaaggtgcgtcggaagctctcgctgctggtggcggtgctgctgttgctgttgctggtggtggtttcggtggtcaacgttctgttgaaccatctgcttactctttcggtgaaatcttaaaagcttggtctccattcttagttttaactgttttagttactatctggactttaaaaccattcaaagctttattcgctccaggtggtgaattatactggttagttttcaacttcgctatcccacacttagatcaattagttgttaaaggtgctccaatcgctacttctgctactccaatcgctgctgttttcaaattcgatccaatctctgctactggtactgctatcttcttatctgctttagtttctatgttcatcttacgtatcggtgctaaaatcggtatcactacttttaaggaagtgctggttgaattaaaatggccaatcttatctatcggtatggttttagctttcgctttcgttactaactactctggtatgtctactactttagctttagttttagctggtactggtgctgctttcccattcttctctccattcttaggttggttaggtgttttcttaactggttctgatacttcttctaacgctttattctcttctttacaatctactactgctcatcaaataggtgtaagcgatactttattagttgctgctaacacttctggtggtgttactggtaaaatgatctctccacaatctatcgctgttgcttgtgctgctactggtatggttggtaaagaatctgatttattccgtttcactttaaaacactctttaatcttcgctgctttagttggtttaatcactttagctcaagcttacttattcactggtatgttagttcactaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z