BBa_K1676099
1
BBa_K1676099
Wildtype Lactate Permease and Lactate Dehydrogenase Operon
2015-09-17T11:00:00Z
2015-09-18T10:45:05Z
Wild type LDP was obtained from a Pseudomonas Strain and then ligated together to obtain this construct
This construct is an operon for expression of wildtype LDH and LDP with pLac as a promoter. However, this in an intermediate part which lacks a transcription terminator.
false
false
_2094_
25361
25361
9
false
-
false
Ooi Kean Hean
component2476058
1
BBa_K1676112
component2476051
1
BBa_R0011
component2476061
1
BBa_K1676097
component2476060
1
BBa_B0034
component2476057
1
BBa_B0034
annotation2476058
1
BBa_K1676112
range2476058
1
82
1227
annotation2476057
1
BBa_B0034
range2476057
1
64
75
annotation2476051
1
BBa_R0011
range2476051
1
1
54
annotation2476061
1
BBa_K1676097
range2476061
1
1254
2942
annotation2476060
1
BBa_B0034
range2476060
1
1236
1247
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1676112
1
BBa_K1676112
Wildtype Lactate Dehydrogenase
2015-09-17T11:00:00Z
2015-09-18T10:33:42Z
-
This enzyme is from the species Psedomonas aeruginosa, which is found to have high lactate oxidizing activity in Shewanella oneidensis MR1 compared to other species. This enzyme catalyses the oxidation of lactate to pyruvate with NAD+ as a cofactor.
false
false
_2094_
25361
25361
9
false
-
false
Ooi Kean Hean
BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation1999
1
lac O1
range1999
1
3
19
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation2000
1
-35
range2000
1
20
25
annotation2001
1
lac O1
range2001
1
26
42
annotation2002
1
-10
range2002
1
43
48
BBa_K1676097
1
BBa_K1676097
Mutant 1 of Lactate Permease
2015-09-17T11:00:00Z
2015-09-18T10:41:25Z
Random mutagenesis was carried out via PCR to obtain the mutants.
Lactate Permease from Pseudomonas. Function in our project is to transport L-lactate into the Shewanella Oneidensis MR1. This mutant contains the mutation of L105V, F120L, V169A, I190K, D183G
false
false
_2094_
25361
25361
9
false
Primers were designed to have the start and stop codon so that they will not be mutated during PCR
false
Ooi Kean Hean
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1676112_sequence
1
atgatcatctctgcttctactgattaccgtgctgctgctcaacgtaaattaccaccattcttattccactacatcgatggtggtgcttacgctgaatacactttacgtcgtaacgttgaagatttatctgctatcgctttacgtcaacgtgttttaaaaaacatgtctgaattatctttagaaactcgtttattcgatgaaactttagctatgccagttgctttagctccagttggtttaactggtatgtacgctcgtcgtggtgaagttcaagctgctcgtgctgctgctgctaaaggtgttccattcactttatctactgtttctgtttgtccaatcgaagaagttgctccagctatcgatcgtccaatgtggttccaattatacgttttaaaagatcgtggtttcatgcgtaacgctttagaacgtgctaaagctgctggtgttactactttagttttcactgttgatatgccagttccaggtgctcgttaccgtgatgctcactctggtatgtctggtccatacgctgctccacgtcgtatcttacaagctatgactcacccagcttgggcttgggatgttggtttattaggcaagccacacgatctcggtaacatctcggcttaccgtggtaacccaactggtttagaagattacatcggttggttaggtgctaacttcgatccatctatctcttggaaagatttagaatggatccgtgagttttgggatggtccaatggttatcaaaggtatcttagatccagaagatgctcgtgatgctgttaaattcggtgctgatggcatagttgtaagcaaccacggtggtcgtcaattagatggtgttttatcttctgctcgtgctttaccagctatcgctgatgctgttaaaggtgaattagctatcttagctgattctggtatccgtactggtttagatgttgttcgtatgatcgctttaggtgctgattctgttttattaggtcgtgctttcgtttacgctttagctgctgctggtgaagctggtgttcgtaacttattagaattaatcgaaaaagaaatgcgtgttgctatggttttaactggtgctaaatctatcggtgaaatctctgctgattctttagttcgtgaattaggtgcttaa
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
BBa_K1676099_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgatcatctctgcttctactgattaccgtgctgctgctcaacgtaaattaccaccattcttattccactacatcgatggtggtgcttacgctgaatacactttacgtcgtaacgttgaagatttatctgctatcgctttacgtcaacgtgttttaaaaaacatgtctgaattatctttagaaactcgtttattcgatgaaactttagctatgccagttgctttagctccagttggtttaactggtatgtacgctcgtcgtggtgaagttcaagctgctcgtgctgctgctgctaaaggtgttccattcactttatctactgtttctgtttgtccaatcgaagaagttgctccagctatcgatcgtccaatgtggttccaattatacgttttaaaagatcgtggtttcatgcgtaacgctttagaacgtgctaaagctgctggtgttactactttagttttcactgttgatatgccagttccaggtgctcgttaccgtgatgctcactctggtatgtctggtccatacgctgctccacgtcgtatcttacaagctatgactcacccagcttgggcttgggatgttggtttattaggcaagccacacgatctcggtaacatctcggcttaccgtggtaacccaactggtttagaagattacatcggttggttaggtgctaacttcgatccatctatctcttggaaagatttagaatggatccgtgagttttgggatggtccaatggttatcaaaggtatcttagatccagaagatgctcgtgatgctgttaaattcggtgctgatggcatagttgtaagcaaccacggtggtcgtcaattagatggtgttttatcttctgctcgtgctttaccagctatcgctgatgctgttaaaggtgaattagctatcttagctgattctggtatccgtactggtttagatgttgttcgtatgatcgctttaggtgctgattctgttttattaggtcgtgctttcgtttacgctttagctgctgctggtgaagctggtgttcgtaacttattagaattaatcgaaaaagaaatgcgtgttgctatggttttaactggtgctaaatctatcggtgaaatctctgctgattctttagttcgtgaattaggtgcttaatactagagaaagaggagaaatactagatgcaagcttggcaacaattatacactccattaggttctttaggtttatctgctgctgctgctgttatcccaatcgttttcttcttcttagctttagctgttttccgtttaaaaggtcacatcgctggttctatcactttagctttagctatcgctgttgctatcttcgctttccacatgcccgctgacatggcgttcgctgctgctggctacggtttcttatacggtttatggccaatcgcttggatcatcgttgctgctgttttcttatacaaattaactgttaaatctggtcaattcgaagttatccgttcttctgttttatctatcactggtgatcaacgtttacaagttttattaatcggtttctctttcggtgctttcttagaaggggcggctggcttcggtgctccagttgctatcactgctgctttattagttggtttaggtttcaacccattatacgctgctggtttatgtttaatcgctaacactgctccagttgctttcggtgctttaggtatcccaatcatcgttgctggtcaagttactggcatagatgcgttcaaaatcggtgcgatgactggtcgtcaattaccattcttatctatcttagttccattctggttagttttcatgatggatggttggcgtggtatccgtgaaacttggccagctgctatcgttgctggtggttctttcgctatcactcaatacttaacttctaacttcatcggtccagaattaccagatatcacttctgctttagtttctttagtttctttaactttattcttaaaagtttggcaaccacaaggtgcgtcggaagctctcgctgctggtggcggtgctgctgttgctgttgctggtggtggtttcggtggtcaacgttctgttgaaccatctgcttactctttcggtgaaatcttaaaagcttggtctccattcttagttttaactgttttagttactatctggactttaaaaccattcaaagctttattcgctccaggtggtgaattatactggttagttttcaacttcgctatcccacacttagatcaattagttgttaaaggtgctccaatcgctacttctgctactccaatcgctgctgttttcaaattcgatccaatctctgctactggtactgctatcttcttatctgctttagtttctatgttcatcttacgtatcggtgctaaaatcggtatcactacttttaaggaagtgctggttgaattaaaatggccaatcttatctatcggtatggttttagctttcgctttcgttactaactactctggtatgtctactactttagctttagttttagctggtactggtgctgctttcccattcttctctccattcttaggttggttaggtgttttcttaactggttctgatacttcttctaacgctttattctcttctttacaatctactactgctcatcaaataggtgtaagcgatactttattagttgctgctaacacttctggtggtgttactggtaaaatgatctctccacaatctatcgctgttgcttgtgctgctactggtatggttggtaaagaatctgatttattccgtttcactttaaaacactctttaatcttcgctgctttagttggtttaatcactttagctcaagcttacttattcactggtatgttagttcactaa
BBa_K1676097_sequence
1
atgcaagcttggcaacaattatacactccattaggttctttaggtttatctgctgctgctgctgttatcccaatcgttttcttcttcttagctttagctgttttccgtttaaaaggtcacatcgctggttctatcactttagctttagctatcgctgttgctatcttcgctttccacatgcccgctgacatggcgttcgctgctgctggctacggtttcttatacggtttatggccaatcgcttggatcatcgttgctgctgttttcttatacaaattaactgttaaatctggtcaattcgaagttatccgttcttctgttttatctatcactggtgatcaacgtttacaagttttattaatcggtttctctttcggtgctttcttagaaggggcggctggcttcggtgctccagttgctatcactgctgctttattagttggtttaggtttcaacccattatacgctgctggtttatgtttaatcgctaacactgctccagttgctttcggtgctttaggtatcccaatcatcgttgctggtcaagttactggcatagatgcgttcaaaatcggtgcgatgactggtcgtcaattaccattcttatctatcttagttccattctggttagttttcatgatggatggttggcgtggtatccgtgaaacttggccagctgctatcgttgctggtggttctttcgctatcactcaatacttaacttctaacttcatcggtccagaattaccagatatcacttctgctttagtttctttagtttctttaactttattcttaaaagtttggcaaccacaaggtgcgtcggaagctctcgctgctggtggcggtgctgctgttgctgttgctggtggtggtttcggtggtcaacgttctgttgaaccatctgcttactctttcggtgaaatcttaaaagcttggtctccattcttagttttaactgttttagttactatctggactttaaaaccattcaaagctttattcgctccaggtggtgaattatactggttagttttcaacttcgctatcccacacttagatcaattagttgttaaaggtgctccaatcgctacttctgctactccaatcgctgctgttttcaaattcgatccaatctctgctactggtactgctatcttcttatctgctttagtttctatgttcatcttacgtatcggtgctaaaatcggtatcactacttttaaggaagtgctggttgaattaaaatggccaatcttatctatcggtatggttttagctttcgctttcgttactaactactctggtatgtctactactttagctttagttttagctggtactggtgctgctttcccattcttctctccattcttaggttggttaggtgttttcttaactggttctgatacttcttctaacgctttattctcttctttacaatctactactgctcatcaaataggtgtaagcgatactttattagttgctgctaacacttctggtggtgttactggtaaaatgatctctccacaatctatcgctgttgcttgtgctgctactggtatggttggtaaagaatctgatttattccgtttcactttaaaacactctttaatcttcgctgctttagttggtttaatcactttagctcaagcttacttattcactggtatgttagttcactaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z