BBa_K1676107 1 BBa_K1676107 Mutant 4 of Lactate Dehydrogenase 2015-09-17T11:00:00Z 2015-09-25T02:21:31Z Random mutagenesis via error prone PCR was carried out on the gene. The is a mutant of lactate dehydrogenase which contain mutation to these positions: S6Y, R10P, Q14K, L60S, A95D, F126L, K132I, R138P, A177T, W193R, W233S, V271F, T311I false false _2094_ 25361 25361 9 false Primers were designed to contain the start and the stop codons so that no mutations would occur on them. false Ooi Kean Hean BBa_K1676107_sequence 1 atgatcatctctgcttatactgattaccctgctgctgctaaacgtaaattaccaccattcttattccactacatcgatggtggtgcttacgctgaatacactttacgtcgtaacgttgaagatttatctgctatcgctttacgtcaacgtgttttaaaaaacatgtctgaattatcttcagaaactcgtttattcgatgaaactttagctatgccagttgctttagctccagttggtttaactggtatgtacgctcgtcgtggtgaagttcaagctgctcgtgatgctgctgctaaaggtgttccattcactttatctactgtttctgtttgtccaatcgaagaagttgctccagctatcgatcgtccaatgtggctccaattatacgttttaatagatcgtggtttcatgcctaacgctttagaacgtgctaaagctgctggtgttactactttagttttcactgttgatatgccagttccaggtgctcgttaccgtgatgctcactctggtatgtctggtccatacactgctccacgtcgtatcttacaagctatgactcacccagcttgggctagggatgttggtttattaggcaagccacacgatctcggtaacatctcggcttaccgtggtaacccaactggtttagaagattacatcggttggttaggtgctaacttcgatccatctatctcttcgaaagatttagaatggatccgtgagttttgggatggtccaatggttatcaaaggtatcttagatccagaagatgctcgtgatgctgttaaattcggtgctgatggcatatttgtaagcaaccacggtggtcgtcaattagatggtgttttatcttctgctcgtgctttaccagctatcgctgatgctgttaaaggtgaattagctatcttagctgattctggtatccgtattggtttagatgttgttcgtatgatcgctttaggtgctgattctgttttattaggtcgtgctttcgtttacgctttagctgctgctggtgaagccggtgttcgtaacttattagaattaatcgaaaaagaaatgcgtgttgctatggttttaactggtgctaaatctatcggtgaaatctctgctgattctttagttcgtgaattaggtgcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z