BBa_K1676108
1
BBa_K1676108
Mutant 5 of Lactate Dehydrogenase
2015-09-17T11:00:00Z
2015-09-18T10:16:37Z
Random mutagenesis via error prone PCR was carried out on the gene.
The is a mutant of lactate dehydrogenase which contain mutation to these positions: R310S
false
false
_2094_
25361
25361
9
false
Primers were designed to contain the start and the stop codons so that no mutations would occur on them.
false
Ooi Kean Hean
BBa_K1676108_sequence
1
atgatcatctctgcttctactgattaccgtgctgctgctcaacgtaaattaccaccattcttattccactacatcgatggtggtgcttacgctgaatacactttacgtcgtaacgttgaagatttatctgctatcgctttacgtcaacgtgttttaaaaaacatgtctgaattatctttagaaactcgtttattcgatgaaactttagctatgccagttgctttagctccagttggtttaactggtatgtacgctcgtcgtggtgaagttcaagctgctcgtgctgctgctgctaaaggtgttccattcactttatctactgtttctgtttgtccaatcgaagaagttgctccagctatcgatcgtccaatgtggttccaattatacgttttaaaagatcgtggtttcatgcgtaacgctttagaacgtgctaaagctgctggtgttactactttagttttcactgttgatatgccagttccaggtgctcgttaccgtgatgctcactctggtatgtctggtccatacgctgctccacgtcgtatcttacaagctatgactcacccagcttgggcttgggatgttggtttattaggcaagccacacgatctcggtaacatctcggcttaccgtggtaacccaactggtttagaagattacatcggttggttaggtgctaacttcgatccatctatctcttggaaagatttagaatggatccgtgagttttgggatggtccaatggttatcaaaggtatcttagatccagaagatgctcgtgatgctgttaaattcggtgctgatggcatagttgtaagcaaccacggtggtcgtcaattagatggtgttttatcttctgctcgtgctttaccagctatcgctgatgctgttaaaggtgaattagctatcttagctgattctggtatcagtactggtttagatgttgttcgtatgatcgctttaggtgctgattctgttttattaggtcgtgctttcgtttacgctttagctgctgctggtgaagctggtgttcgtaacttattagaattaatcgaaaaagaaatgcgtgttgctatggttttaactggtgctaaatctatcggtgaaatctctgctgattctttagttcgtgaattaggtgcttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z