BBa_K1676110 1 BBa_K1676110 Mutant 7 of Lactate Dehydrogenase 2015-09-17T11:00:00Z 2015-09-18T10:19:10Z Random mutagenesis via error prone PCR was carried out on the gene. The is a mutant of lactate dehydrogenase which contain mutation to these positions: R10H, A31T, I113F, P159L, S170Y, K200M false false _2094_ 25361 25361 9 false Primers were designed to contain the start and the stop codons so that no mutations would occur on them. false Ooi Kean Hean BBa_K1676110_sequence 1 atgatcatctctgcttctactgattaccatgctgctgctcaacgtaaattaccaccattcttattccactacatcgatggtggtgcttacactgaatacactttgcgtcgtaacgttgaagatttatctgctatcgctttacgtcaacgtgttttaaaaaacatgtctgaattatctttagaaactcgtttattcgatgaaactttagctatgccagttgctttagctccagttggtttaactggtatgtacgctcgtcgtggtgaagttcaagctgctcgtgctgctgctgctaaaggtgttccattcactttatctactgtttctgtttgtccattcgaagaagttgctccagctatcgatcgtccaatgtggttccaattatacgttttaaaagatcgtggtttcatgcgtaacgctttagaacgtgctaaagctgctggtgttactactttagttttcactgttgatatgctagttccaggtgctcgttaccgtgatgctcactatggtatgtctggtccatacgctgctccacgtcgtatcttacaagctatgactcacccagcttgggcttgggatgttggtttattaggcatgccacacgatctcggtaacatctcggcttaccgtggtaacccaactggtttagaagattacatcggttggttaggtgctaacttcgatccatctatctcttggaaagatttagaatggatccgtgagttttgggatggtccaatggttatcaaaggtatcttagatccagaagatgctcgtgatgctgttaaattcggtgctgatggcatagttgtaagcaaccacggtggtcgtcaattagatggtgttttatcttctgctcgtgctttaccagctatcgctgatgctgttaaaggtgaattagctatcttagctgattctggtatccgtactggtttagatgttgttcgtatgatcgctttaggtgctgattctgttttattaggtcgtgctttcgtttacgctttagctgctgctggtgaagctggtgttcgtaacttattagaattaatcgaaaaagaaatgcgtgttgctatggttttaactggtgctaaatctatcggtgaaatctctgctgattctttagttcgtgaattaggtgctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z