LOCUS       BBa_K1677000             460 bp    DNA     linear       23-Dec-2024
DEFINITION  IPTG Inducible Tsi2 Antitoxin
ACCESSION   BBa_K1677000
VERSION     BBa_K1677000.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..460
                     /label=tsi2
     CDS             227..460
                     /label=BBa_K314201
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga acctgaaacc
      241 ccagaccctg atggtggcga tccagtgcgt cgccgcgcgc acccgcgaac tcgacgcgca
      301 gttgcagaac gacgacccgc agaacgccgc tgaactcgaa cagttgctgg tcggctacga
      361 cctcgccgcc gacgacctga agaacgccta cgagcaagcc ctgggccaat acagcggcct
      421 gccgccctat gaccggctga tcgaagagcc ggcatcctga
//