LOCUS       BBa_K1677001            1344 bp    DNA     linear       16-Jun-2024
DEFINITION  IPTG Inducible Tsi2 Antitoxin with a GFP reporter
ACCESSION   BBa_K1677001
VERSION     BBa_K1677001.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..460
                     /label=tsi2
     CDS             227..460
                     /label=BBa_K314201
     misc_feature    1..460
                     /label=BBa_K1677000
     RBS             469..481
                     /label=RBS-3\Weak
     RBS             475..478
                     /label=RBS
     misc_feature    469..481
                     /label=BBa_B0032
     RBS             469..481
                     /label=BBa_B0032
     CDS             488..1207
                     /label=GFP protein
     CDS             488..1207
                     /label=BBa_E0040
     stem_loop       1227..1270
                     /label=stem_loop
     misc_feature    1216..1295
                     /label=BBa_B0010
     terminator      1216..1295
                     /label=BBa_B0010
     stem_loop       1311..1330
                     /label=T7 TE
     misc_feature    1337..1337
                     /label=stop
     polyA_site      1331..1344
                     /label=polya
     misc_feature    1304..1344
                     /label=BBa_B0012
     terminator      1304..1344
                     /label=BBa_B0012
     misc_feature    469..1344
                     /label=BBa_E0240
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga acctgaaacc
      241 ccagaccctg atggtggcga tccagtgcgt cgccgcgcgc acccgcgaac tcgacgcgca
      301 gttgcagaac gacgacccgc agaacgccgc tgaactcgaa cagttgctgg tcggctacga
      361 cctcgccgcc gacgacctga agaacgccta cgagcaagcc ctgggccaat acagcggcct
      421 gccgccctat gaccggctga tcgaagagcc ggcatcctga tactagagtc acacaggaaa
      481 gtactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      541 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      601 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
      661 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
      721 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
      781 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
      841 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
      901 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
      961 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
     1021 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     1081 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
     1141 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     1201 ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1261 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1321 cgggtgggcc tttctgcgtt tata
//