##gff-version 3
##sequence-region BBa_K1677001 1 1344
BBa_K1677001	.	engineered_region	469	1344	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K1677001	.	terminator	1304	1344	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K1677001	.	polyA_site	1331	1344	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1677001	.	engineered_region	1304	1344	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1677001	.	stop_codon	1337	1337	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1677001	.	stem_loop	1311	1330	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1677001	.	CDS	488	1207	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K1677001	.	CDS	488	1207	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1677001	.	terminator	1216	1295	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K1677001	.	engineered_region	1216	1295	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1677001	.	stem_loop	1227	1270	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1677001	.	ribosome_entry_site	469	481	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K1677001	.	engineered_region	469	481	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1677001	.	ribosome_entry_site	475	478	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1677001	.	ribosome_entry_site	469	481	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1677001	.	engineered_region	1	460	.	+	0	ID=BBa_K1677000;Name=BBa_K1677000
BBa_K1677001	.	CDS	227	460	.	+	0	ID=BBa_K314201;Name=BBa_K314201;Parent=BBa_K1677000
BBa_K1677001	.	engineered_region	227	460	.	+	0	ID=annotation2089457;Name=tsi2;Parent=BBa_K314201
BBa_K1677001	.	engineered_region	1	220	.	+	0	ID=BBa_J04500;Name=BBa_J04500;Parent=BBa_K1677000
BBa_K1677001	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K1677001	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1677001	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K1677001	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1677001	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1677001	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1677001	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1677001	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1677001	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1677001	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
>BBa_K1677001
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaacctgaa
accccagaccctgatggtggcgatccagtgcgtcgccgcgcgcacccgcgaactcgacgcgcagttgcagaacgacgac
ccgcagaacgccgctgaactcgaacagttgctggtcggctacgacctcgccgccgacgacctgaagaacgcctacgagc
aagccctgggccaatacagcggcctgccgccctatgaccggctgatcgaagagccggcatcctgatactagagtcacac
aggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgtt
aatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgca
ctactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagataccc
agatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaa
gatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggta
ttgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggc
agacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagac
cattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccc
tttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcat
ggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
a