LOCUS       BBa_K1677010            2757 bp    DNA     linear       25-Nov-2024
DEFINITION  IPTG inducible invasin
ACCESSION   BBa_K1677010
VERSION     BBa_K1677010.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     RBS             229..241
                     /label=RBS-3\Weak
     RBS             235..238
                     /label=RBS
     misc_feature    229..241
                     /label=BBa_B0032
     RBS             229..241
                     /label=BBa_B0032
     CDS             250..3075
                     /label=protein
     CDS             250..2757
                     /label=BBa_K177010
     misc_feature    229..2757
                     /label=BBa_K299810
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagtc acacaggaaa
      241 gtactagagc tattgaggct ccgcacacag cggatacgcc gtagccgcag atgttggtat
      301 gtcgccggtg gtcatatcca tagtgacaaa atctgtactg gtctttttag tccaatagtt
      361 acccgatggc cactcagcgc tatcataggt tgccaaactt ccccactctc cccacagagt
      421 cccgtctggt gaacgcgaac cattactggc gcgtgcggac tcgatcagcg cagtaaaatc
      481 agtgcgctgg caatttcgac tcgcctcgac acttgattgc agtgacatgg tgccggagaa
      541 cacccacaaa ttaggtttga attgatatgt tgccgtataa ctcgggaatt ttttactttt
      601 tgccgtcaca gtgacggtgc taccataggt tttataggca atattgactt taccctgatt
      661 atcaaccggc gcactggccg cataggatga tgtccagtca tattgagtat tattcgccac
      721 atcgtcattc atcaccaatt ggaacgtggc tttattaaag gtagttttcg ggaagccttt
      781 atctgtggca aattgctcac cattcacctt aatgccggtt atcttcggta ctgggtacag
      841 ggtgattctt ttctgcaaca agtctagtga ttcccccccc acctgcggcg taatatcgac
      901 atctcctacg gaatttccca ccacactggc ggtatagttg tcagcatttt cggttaccga
      961 actaatagtt accggaacac cactttgtat aaattcaaga tctgttatcc cactgacaaa
     1021 ctcattattt ttattacgcg gaacaaaggt gagtatcgac tgcatggagc catcggcaac
     1081 aatatcagat ggagaaacgt taaaacttga gtttgtgaca tctggcggca gcacggcaaa
     1141 tcgtacatca acagtagcct gttgcccctg aatatctaaa gtgacgactg acaccccaac
     1201 agtgaagcta gttaatgcaa tgcttatcac accctgtgca tcggtttttt ttgtgacttt
     1261 actgggaagc gcaccattac tggtggttat caccccctct tgctcggcaa ccggcgtact
     1321 atcaatatta gtcactgtaa atttcaccgt gatgggagtc ctgccatcag caagcgcacc
     1381 atcatcagtc acttccgagg taataacgaa ctgaggttgc tgcacaataa cggtcaacgt
     1441 agctggatta gaactattgc cgtggttatc gatagccgtg gcgctgaggg tataggtatt
     1501 gaccgccgct tcgctctcgc cggttttccc cacagtacga ctcgctggtg ccatcggttt
     1561 ataaggcggt aagaccacat tgtaatctgt tgcactgacg ggtattaatt tgccgccggc
     1621 agcaacccat tgcgcatcat tccagagaat acgttgtagt gcagattgag actgtatttg
     1681 tgcactaacg gaataaacct gccccggcag gccggagagt acggcgggtg aaaatgccaa
     1741 tttgatagta ttctgttttt ggtattccag aacaatattt ggattgcgct caaccagatt
     1801 ataacggctc tcagccagta aacgagttcc ggccaccact gcggggctaa actgcgaacg
     1861 aaaactttcg ccgaggcgat aatccatttg taagttccat tgtatttcat gctgccgact
     1921 ttttcccatt cgttgatcga cccccagtgt aatgaagggg atcggcgtat aaataagccc
     1981 tgtggtcacc gcataagggt tactttgcag gttatcttta ccaaataaag ccacccgctc
     2041 accacggtac tgctcatatt ttaatttccc gcccaattgt ggcagcgcag gtaaatacgc
     2101 tttgacgtga atgtcgcccc cgcttgccgg gcgctcatta tagtccgcga aatcacgaga
     2161 ttgatgccaa ccattgaggc gaaaataacc attggccgat aattgtaaat aatcagtcca
     2221 ggcttctgca cccacgccaa tacgatgatt gtgcccggtc atatcattgt catagaaagt
     2281 gttaaagcca tacatccaac tttgttggaa ggtacgcacc ccagcgccga tattaagggt
     2341 attgcgactg tctttatttc ttatacccaa ctgactaaaa aagacatatg aagcagagtc
     2401 ataccaaggc aacagccaat caagagaact ttctttgagg gagaactttt tatcaaagtt
     2461 aacattgacc tgagttgtcc caaatctatt taaccagtgt tttacttcct gatttgccgc
     2521 gtcatttacc attgacctgg tgatattgga tgcattaccc gataccgcca gactggttcc
     2581 ggtactgatt atcttattga aagcctcatt atgttcagcg gggttctcta acgcttctga
     2641 attaaaataa tgttgctgtg agaacccata agtaaatatt ccaaatatta aaccgatcga
     2701 taatattagc ctgctaatta ttttagtcac agttagcgta ttaaaaaatg aatacat
//