BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961225 1 -10 range1961225 1 161 166 annotation1961227 1 start range1961227 1 173 173 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961224 1 -35 range1961224 1 137 142 BBa_K177010 1 BBa_K177010 invasine locus from yersinia 2009-07-06T11:00:00Z 2015-05-08T01:11:05Z Genomic DNA from Yersinia enable bacteria to enter a cell false false _284_ 0 4696 9 Not in stock false none false Kamila Ornoch annotation2010434 1 protein range2010434 1 1 2826 BBa_J04500 1 BBa_J04500 IPTG inducible promoter with RBS 2005-06-08T11:00:00Z 2015-08-31T04:08:14Z Davidson Synth-Aces Released HQ 2013 R0010.B0034 false true _16_ 0 326 16 In stock false false Kristen DeCelle component1508149 1 BBa_R0010 component1508159 1 BBa_B0034 annotation1508159 1 BBa_B0034 range1508159 1 209 220 annotation1508149 1 BBa_R0010 range1508149 1 1 200 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K299810 1 BBa_K299810 B0032+Invasin 2010-10-12T11:00:00Z 2015-05-08T01:11:50Z false false _419_ 0 7677 9 It's complicated true false Micha&#322; Lower component2085758 1 BBa_K177010 component2085755 1 BBa_B0032 annotation2085755 1 BBa_B0032 range2085755 1 1 13 annotation2085758 1 BBa_K177010 range2085758 1 22 2529 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1710 1 RBS range1710 1 7 10 annotation1709 1 RBS-3\Weak range1709 1 1 13 BBa_K1677010 1 BBa_K1677010 IPTG inducible invasin 2015-07-14T11:00:00Z 2015-07-26T08:11:47Z The gene is from Yersinia pestis The invasin gene under the control of a IPTG inducible promoter. false false _2095_ 24918 25020 9 false - false Manan Shah, Isabelle Capell-Hattam component2433142 1 BBa_J04500 component2433148 1 BBa_K299810 annotation2433148 1 BBa_K299810 range2433148 1 229 2757 annotation2433142 1 BBa_J04500 range2433142 1 1 220 BBa_K299810_sequence 1 tcacacaggaaagtactagagctattgaggctccgcacacagcggatacgccgtagccgcagatgttggtatgtcgccggtggtcatatccatagtgacaaaatctgtactggtctttttagtccaatagttacccgatggccactcagcgctatcataggttgccaaacttccccactctccccacagagtcccgtctggtgaacgcgaaccattactggcgcgtgcggactcgatcagcgcagtaaaatcagtgcgctggcaatttcgactcgcctcgacacttgattgcagtgacatggtgccggagaacacccacaaattaggtttgaattgatatgttgccgtataactcgggaattttttactttttgccgtcacagtgacggtgctaccataggttttataggcaatattgactttaccctgattatcaaccggcgcactggccgcataggatgatgtccagtcatattgagtattattcgccacatcgtcattcatcaccaattggaacgtggctttattaaaggtagttttcgggaagcctttatctgtggcaaattgctcaccattcaccttaatgccggttatcttcggtactgggtacagggtgattcttttctgcaacaagtctagtgattccccccccacctgcggcgtaatatcgacatctcctacggaatttcccaccacactggcggtatagttgtcagcattttcggttaccgaactaatagttaccggaacaccactttgtataaattcaagatctgttatcccactgacaaactcattatttttattacgcggaacaaaggtgagtatcgactgcatggagccatcggcaacaatatcagatggagaaacgttaaaacttgagtttgtgacatctggcggcagcacggcaaatcgtacatcaacagtagcctgttgcccctgaatatctaaagtgacgactgacaccccaacagtgaagctagttaatgcaatgcttatcacaccctgtgcatcggttttttttgtgactttactgggaagcgcaccattactggtggttatcaccccctcttgctcggcaaccggcgtactatcaatattagtcactgtaaatttcaccgtgatgggagtcctgccatcagcaagcgcaccatcatcagtcacttccgaggtaataacgaactgaggttgctgcacaataacggtcaacgtagctggattagaactattgccgtggttatcgatagccgtggcgctgagggtataggtattgaccgccgcttcgctctcgccggttttccccacagtacgactcgctggtgccatcggtttataaggcggtaagaccacattgtaatctgttgcactgacgggtattaatttgccgccggcagcaacccattgcgcatcattccagagaatacgttgtagtgcagattgagactgtatttgtgcactaacggaataaacctgccccggcaggccggagagtacggcgggtgaaaatgccaatttgatagtattctgtttttggtattccagaacaatatttggattgcgctcaaccagattataacggctctcagccagtaaacgagttccggccaccactgcggggctaaactgcgaacgaaaactttcgccgaggcgataatccatttgtaagttccattgtatttcatgctgccgactttttcccattcgttgatcgacccccagtgtaatgaaggggatcggcgtataaataagccctgtggtcaccgcataagggttactttgcaggttatctttaccaaataaagccacccgctcaccacggtactgctcatattttaatttcccgcccaattgtggcagcgcaggtaaatacgctttgacgtgaatgtcgcccccgcttgccgggcgctcattatagtccgcgaaatcacgagattgatgccaaccattgaggcgaaaataaccattggccgataattgtaaataatcagtccaggcttctgcacccacgccaatacgatgattgtgcccggtcatatcattgtcatagaaagtgttaaagccatacatccaactttgttggaaggtacgcaccccagcgccgatattaagggtattgcgactgtctttatttcttatacccaactgactaaaaaagacatatgaagcagagtcataccaaggcaacagccaatcaagagaactttctttgagggagaactttttatcaaagttaacattgacctgagttgtcccaaatctatttaaccagtgttttacttcctgatttgccgcgtcatttaccattgacctggtgatattggatgcattacccgataccgccagactggttccggtactgattatcttattgaaagcctcattatgttcagcggggttctctaacgcttctgaattaaaataatgttgctgtgagaacccataagtaaatattccaaatattaaaccgatcgataatattagcctgctaattattttagtcacagttagcgtattaaaaaatgaatacat BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_J04500_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa BBa_B0032_sequence 1 tcacacaggaaag BBa_K1677010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagtcacacaggaaagtactagagctattgaggctccgcacacagcggatacgccgtagccgcagatgttggtatgtcgccggtggtcatatccatagtgacaaaatctgtactggtctttttagtccaatagttacccgatggccactcagcgctatcataggttgccaaacttccccactctccccacagagtcccgtctggtgaacgcgaaccattactggcgcgtgcggactcgatcagcgcagtaaaatcagtgcgctggcaatttcgactcgcctcgacacttgattgcagtgacatggtgccggagaacacccacaaattaggtttgaattgatatgttgccgtataactcgggaattttttactttttgccgtcacagtgacggtgctaccataggttttataggcaatattgactttaccctgattatcaaccggcgcactggccgcataggatgatgtccagtcatattgagtattattcgccacatcgtcattcatcaccaattggaacgtggctttattaaaggtagttttcgggaagcctttatctgtggcaaattgctcaccattcaccttaatgccggttatcttcggtactgggtacagggtgattcttttctgcaacaagtctagtgattccccccccacctgcggcgtaatatcgacatctcctacggaatttcccaccacactggcggtatagttgtcagcattttcggttaccgaactaatagttaccggaacaccactttgtataaattcaagatctgttatcccactgacaaactcattatttttattacgcggaacaaaggtgagtatcgactgcatggagccatcggcaacaatatcagatggagaaacgttaaaacttgagtttgtgacatctggcggcagcacggcaaatcgtacatcaacagtagcctgttgcccctgaatatctaaagtgacgactgacaccccaacagtgaagctagttaatgcaatgcttatcacaccctgtgcatcggttttttttgtgactttactgggaagcgcaccattactggtggttatcaccccctcttgctcggcaaccggcgtactatcaatattagtcactgtaaatttcaccgtgatgggagtcctgccatcagcaagcgcaccatcatcagtcacttccgaggtaataacgaactgaggttgctgcacaataacggtcaacgtagctggattagaactattgccgtggttatcgatagccgtggcgctgagggtataggtattgaccgccgcttcgctctcgccggttttccccacagtacgactcgctggtgccatcggtttataaggcggtaagaccacattgtaatctgttgcactgacgggtattaatttgccgccggcagcaacccattgcgcatcattccagagaatacgttgtagtgcagattgagactgtatttgtgcactaacggaataaacctgccccggcaggccggagagtacggcgggtgaaaatgccaatttgatagtattctgtttttggtattccagaacaatatttggattgcgctcaaccagattataacggctctcagccagtaaacgagttccggccaccactgcggggctaaactgcgaacgaaaactttcgccgaggcgataatccatttgtaagttccattgtatttcatgctgccgactttttcccattcgttgatcgacccccagtgtaatgaaggggatcggcgtataaataagccctgtggtcaccgcataagggttactttgcaggttatctttaccaaataaagccacccgctcaccacggtactgctcatattttaatttcccgcccaattgtggcagcgcaggtaaatacgctttgacgtgaatgtcgcccccgcttgccgggcgctcattatagtccgcgaaatcacgagattgatgccaaccattgaggcgaaaataaccattggccgataattgtaaataatcagtccaggcttctgcacccacgccaatacgatgattgtgcccggtcatatcattgtcatagaaagtgttaaagccatacatccaactttgttggaaggtacgcaccccagcgccgatattaagggtattgcgactgtctttatttcttatacccaactgactaaaaaagacatatgaagcagagtcataccaaggcaacagccaatcaagagaactttctttgagggagaactttttatcaaagttaacattgacctgagttgtcccaaatctatttaaccagtgttttacttcctgatttgccgcgtcatttaccattgacctggtgatattggatgcattacccgataccgccagactggttccggtactgattatcttattgaaagcctcattatgttcagcggggttctctaacgcttctgaattaaaataatgttgctgtgagaacccataagtaaatattccaaatattaaaccgatcgataatattagcctgctaattattttagtcacagttagcgtattaaaaaatgaatacat BBa_K177010_sequence 1 ctattgaggctccgcacacagcggatacgccgtagccgcagatgttggtatgtcgccggtggtcatatccatagtgacaaaatctgtactggtctttttagtccaatagttacccgatggccactcagcgctatcataggttgccaaacttccccactctccccacagagtcccgtctggtgaacgcgaaccattactggcgcgtgcggactcgatcagcgcagtaaaatcagtgcgctggcaatttcgactcgcctcgacacttgattgcagtgacatggtgccggagaacacccacaaattaggtttgaattgatatgttgccgtataactcgggaattttttactttttgccgtcacagtgacggtgctaccataggttttataggcaatattgactttaccctgattatcaaccggcgcactggccgcataggatgatgtccagtcatattgagtattattcgccacatcgtcattcatcaccaattggaacgtggctttattaaaggtagttttcgggaagcctttatctgtggcaaattgctcaccattcaccttaatgccggttatcttcggtactgggtacagggtgattcttttctgcaacaagtctagtgattccccccccacctgcggcgtaatatcgacatctcctacggaatttcccaccacactggcggtatagttgtcagcattttcggttaccgaactaatagttaccggaacaccactttgtataaattcaagatctgttatcccactgacaaactcattatttttattacgcggaacaaaggtgagtatcgactgcatggagccatcggcaacaatatcagatggagaaacgttaaaacttgagtttgtgacatctggcggcagcacggcaaatcgtacatcaacagtagcctgttgcccctgaatatctaaagtgacgactgacaccccaacagtgaagctagttaatgcaatgcttatcacaccctgtgcatcggttttttttgtgactttactgggaagcgcaccattactggtggttatcaccccctcttgctcggcaaccggcgtactatcaatattagtcactgtaaatttcaccgtgatgggagtcctgccatcagcaagcgcaccatcatcagtcacttccgaggtaataacgaactgaggttgctgcacaataacggtcaacgtagctggattagaactattgccgtggttatcgatagccgtggcgctgagggtataggtattgaccgccgcttcgctctcgccggttttccccacagtacgactcgctggtgccatcggtttataaggcggtaagaccacattgtaatctgttgcactgacgggtattaatttgccgccggcagcaacccattgcgcatcattccagagaatacgttgtagtgcagattgagactgtatttgtgcactaacggaataaacctgccccggcaggccggagagtacggcgggtgaaaatgccaatttgatagtattctgtttttggtattccagaacaatatttggattgcgctcaaccagattataacggctctcagccagtaaacgagttccggccaccactgcggggctaaactgcgaacgaaaactttcgccgaggcgataatccatttgtaagttccattgtatttcatgctgccgactttttcccattcgttgatcgacccccagtgtaatgaaggggatcggcgtataaataagccctgtggtcaccgcataagggttactttgcaggttatctttaccaaataaagccacccgctcaccacggtactgctcatattttaatttcccgcccaattgtggcagcgcaggtaaatacgctttgacgtgaatgtcgcccccgcttgccgggcgctcattatagtccgcgaaatcacgagattgatgccaaccattgaggcgaaaataaccattggccgataattgtaaataatcagtccaggcttctgcacccacgccaatacgatgattgtgcccggtcatatcattgtcatagaaagtgttaaagccatacatccaactttgttggaaggtacgcaccccagcgccgatattaagggtattgcgactgtctttatttcttatacccaactgactaaaaaagacatatgaagcagagtcataccaaggcaacagccaatcaagagaactttctttgagggagaactttttatcaaagttaacattgacctgagttgtcccaaatctatttaaccagtgttttacttcctgatttgccgcgtcatttaccattgacctggtgatattggatgcattacccgataccgccagactggttccggtactgattatcttattgaaagcctcattatgttcagcggggttctctaacgcttctgaattaaaataatgttgctgtgagaacccataagtaaatattccaaatattaaaccgatcgataatattagcctgctaattattttagtcacagttagcgtattaaaaaatgaatacat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z