LOCUS BBa_K1677011 3641 bp DNA linear 22-Nov-2024 DEFINITION IPTG inducible invasin with a GFP reporter ACCESSION BBa_K1677011 VERSION BBa_K1677011.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 1..220 /label=BBa_J04500 RBS 229..241 /label=RBS-3\Weak RBS 235..238 /label=RBS misc_feature 229..241 /label=BBa_B0032 RBS 229..241 /label=BBa_B0032 CDS 250..3075 /label=protein CDS 250..2757 /label=BBa_K177010 misc_feature 229..2757 /label=BBa_K299810 RBS 2766..2778 /label=RBS-3\Weak RBS 2772..2775 /label=RBS misc_feature 2766..2778 /label=BBa_B0032 RBS 2766..2778 /label=BBa_B0032 CDS 2785..3504 /label=GFP protein CDS 2785..3504 /label=BBa_E0040 stem_loop 3524..3567 /label=stem_loop misc_feature 3513..3592 /label=BBa_B0010 terminator 3513..3592 /label=BBa_B0010 stem_loop 3608..3627 /label=T7 TE misc_feature 3634..3634 /label=stop polyA_site 3628..3641 /label=polya misc_feature 3601..3641 /label=BBa_B0012 terminator 3601..3641 /label=BBa_B0012 misc_feature 2766..3641 /label=BBa_E0240 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagtc acacaggaaa 241 gtactagagc tattgaggct ccgcacacag cggatacgcc gtagccgcag atgttggtat 301 gtcgccggtg gtcatatcca tagtgacaaa atctgtactg gtctttttag tccaatagtt 361 acccgatggc cactcagcgc tatcataggt tgccaaactt ccccactctc cccacagagt 421 cccgtctggt gaacgcgaac cattactggc gcgtgcggac tcgatcagcg cagtaaaatc 481 agtgcgctgg caatttcgac tcgcctcgac acttgattgc agtgacatgg tgccggagaa 541 cacccacaaa ttaggtttga attgatatgt tgccgtataa ctcgggaatt ttttactttt 601 tgccgtcaca gtgacggtgc taccataggt tttataggca atattgactt taccctgatt 661 atcaaccggc gcactggccg cataggatga tgtccagtca tattgagtat tattcgccac 721 atcgtcattc atcaccaatt ggaacgtggc tttattaaag gtagttttcg ggaagccttt 781 atctgtggca aattgctcac cattcacctt aatgccggtt atcttcggta ctgggtacag 841 ggtgattctt ttctgcaaca agtctagtga ttcccccccc acctgcggcg taatatcgac 901 atctcctacg gaatttccca ccacactggc ggtatagttg tcagcatttt cggttaccga 961 actaatagtt accggaacac cactttgtat aaattcaaga tctgttatcc cactgacaaa 1021 ctcattattt ttattacgcg gaacaaaggt gagtatcgac tgcatggagc catcggcaac 1081 aatatcagat ggagaaacgt taaaacttga gtttgtgaca tctggcggca gcacggcaaa 1141 tcgtacatca acagtagcct gttgcccctg aatatctaaa gtgacgactg acaccccaac 1201 agtgaagcta gttaatgcaa tgcttatcac accctgtgca tcggtttttt ttgtgacttt 1261 actgggaagc gcaccattac tggtggttat caccccctct tgctcggcaa ccggcgtact 1321 atcaatatta gtcactgtaa atttcaccgt gatgggagtc ctgccatcag caagcgcacc 1381 atcatcagtc acttccgagg taataacgaa ctgaggttgc tgcacaataa cggtcaacgt 1441 agctggatta gaactattgc cgtggttatc gatagccgtg gcgctgaggg tataggtatt 1501 gaccgccgct tcgctctcgc cggttttccc cacagtacga ctcgctggtg ccatcggttt 1561 ataaggcggt aagaccacat tgtaatctgt tgcactgacg ggtattaatt tgccgccggc 1621 agcaacccat tgcgcatcat tccagagaat acgttgtagt gcagattgag actgtatttg 1681 tgcactaacg gaataaacct gccccggcag gccggagagt acggcgggtg aaaatgccaa 1741 tttgatagta ttctgttttt ggtattccag aacaatattt ggattgcgct caaccagatt 1801 ataacggctc tcagccagta aacgagttcc ggccaccact gcggggctaa actgcgaacg 1861 aaaactttcg ccgaggcgat aatccatttg taagttccat tgtatttcat gctgccgact 1921 ttttcccatt cgttgatcga cccccagtgt aatgaagggg atcggcgtat aaataagccc 1981 tgtggtcacc gcataagggt tactttgcag gttatcttta ccaaataaag ccacccgctc 2041 accacggtac tgctcatatt ttaatttccc gcccaattgt ggcagcgcag gtaaatacgc 2101 tttgacgtga atgtcgcccc cgcttgccgg gcgctcatta tagtccgcga aatcacgaga 2161 ttgatgccaa ccattgaggc gaaaataacc attggccgat aattgtaaat aatcagtcca 2221 ggcttctgca cccacgccaa tacgatgatt gtgcccggtc atatcattgt catagaaagt 2281 gttaaagcca tacatccaac tttgttggaa ggtacgcacc ccagcgccga tattaagggt 2341 attgcgactg tctttatttc ttatacccaa ctgactaaaa aagacatatg aagcagagtc 2401 ataccaaggc aacagccaat caagagaact ttctttgagg gagaactttt tatcaaagtt 2461 aacattgacc tgagttgtcc caaatctatt taaccagtgt tttacttcct gatttgccgc 2521 gtcatttacc attgacctgg tgatattgga tgcattaccc gataccgcca gactggttcc 2581 ggtactgatt atcttattga aagcctcatt atgttcagcg gggttctcta acgcttctga 2641 attaaaataa tgttgctgtg agaacccata agtaaatatt ccaaatatta aaccgatcga 2701 taatattagc ctgctaatta ttttagtcac agttagcgta ttaaaaaatg aatacattac 2761 tagagtcaca caggaaagta ctagatgcgt aaaggagaag aacttttcac tggagttgtc 2821 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag 2881 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa 2941 ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg 3001 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat 3061 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc 3121 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa 3181 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc 3241 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa 3301 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct 3361 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac 3421 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc 3481 atggatgaac tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca 3541 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 3601 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //