##gff-version 3
##sequence-region BBa_K1677011 1 3641
BBa_K1677011	.	engineered_region	1	220	.	+	0	ID=BBa_J04500;Name=BBa_J04500
BBa_K1677011	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K1677011	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1677011	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1677011	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1677011	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1677011	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1677011	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1677011	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1677011	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K1677011	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1677011	.	engineered_region	229	2757	.	+	0	ID=BBa_K299810;Name=BBa_K299810
BBa_K1677011	.	ribosome_entry_site	229	241	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K299810
BBa_K1677011	.	ribosome_entry_site	235	238	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1677011	.	ribosome_entry_site	229	241	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1677011	.	engineered_region	229	241	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1677011	.	CDS	250	2757	.	+	0	ID=BBa_K177010;Name=BBa_K177010;Parent=BBa_K299810
BBa_K1677011	.	CDS	250	3075	.	+	0	ID=annotation2010434;Name=protein;Parent=BBa_K177010
BBa_K1677011	.	engineered_region	2766	3641	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K1677011	.	terminator	3513	3592	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K1677011	.	stem_loop	3524	3567	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1677011	.	engineered_region	3513	3592	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1677011	.	CDS	2785	3504	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K1677011	.	CDS	2785	3504	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1677011	.	terminator	3601	3641	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K1677011	.	polyA_site	3628	3641	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1677011	.	stem_loop	3608	3627	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1677011	.	engineered_region	3601	3641	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1677011	.	stop_codon	3634	3634	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1677011	.	ribosome_entry_site	2766	2778	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K1677011	.	ribosome_entry_site	2772	2775	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1677011	.	ribosome_entry_site	2766	2778	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1677011	.	engineered_region	2766	2778	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
>BBa_K1677011
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagtcacacagg
aaagtactagagctattgaggctccgcacacagcggatacgccgtagccgcagatgttggtatgtcgccggtggtcata
tccatagtgacaaaatctgtactggtctttttagtccaatagttacccgatggccactcagcgctatcataggttgcca
aacttccccactctccccacagagtcccgtctggtgaacgcgaaccattactggcgcgtgcggactcgatcagcgcagt
aaaatcagtgcgctggcaatttcgactcgcctcgacacttgattgcagtgacatggtgccggagaacacccacaaatta
ggtttgaattgatatgttgccgtataactcgggaattttttactttttgccgtcacagtgacggtgctaccataggttt
tataggcaatattgactttaccctgattatcaaccggcgcactggccgcataggatgatgtccagtcatattgagtatt
attcgccacatcgtcattcatcaccaattggaacgtggctttattaaaggtagttttcgggaagcctttatctgtggca
aattgctcaccattcaccttaatgccggttatcttcggtactgggtacagggtgattcttttctgcaacaagtctagtg
attccccccccacctgcggcgtaatatcgacatctcctacggaatttcccaccacactggcggtatagttgtcagcatt
ttcggttaccgaactaatagttaccggaacaccactttgtataaattcaagatctgttatcccactgacaaactcatta
tttttattacgcggaacaaaggtgagtatcgactgcatggagccatcggcaacaatatcagatggagaaacgttaaaac
ttgagtttgtgacatctggcggcagcacggcaaatcgtacatcaacagtagcctgttgcccctgaatatctaaagtgac
gactgacaccccaacagtgaagctagttaatgcaatgcttatcacaccctgtgcatcggttttttttgtgactttactg
ggaagcgcaccattactggtggttatcaccccctcttgctcggcaaccggcgtactatcaatattagtcactgtaaatt
tcaccgtgatgggagtcctgccatcagcaagcgcaccatcatcagtcacttccgaggtaataacgaactgaggttgctg
cacaataacggtcaacgtagctggattagaactattgccgtggttatcgatagccgtggcgctgagggtataggtattg
accgccgcttcgctctcgccggttttccccacagtacgactcgctggtgccatcggtttataaggcggtaagaccacat
tgtaatctgttgcactgacgggtattaatttgccgccggcagcaacccattgcgcatcattccagagaatacgttgtag
tgcagattgagactgtatttgtgcactaacggaataaacctgccccggcaggccggagagtacggcgggtgaaaatgcc
aatttgatagtattctgtttttggtattccagaacaatatttggattgcgctcaaccagattataacggctctcagcca
gtaaacgagttccggccaccactgcggggctaaactgcgaacgaaaactttcgccgaggcgataatccatttgtaagtt
ccattgtatttcatgctgccgactttttcccattcgttgatcgacccccagtgtaatgaaggggatcggcgtataaata
agccctgtggtcaccgcataagggttactttgcaggttatctttaccaaataaagccacccgctcaccacggtactgct
catattttaatttcccgcccaattgtggcagcgcaggtaaatacgctttgacgtgaatgtcgcccccgcttgccgggcg
ctcattatagtccgcgaaatcacgagattgatgccaaccattgaggcgaaaataaccattggccgataattgtaaataa
tcagtccaggcttctgcacccacgccaatacgatgattgtgcccggtcatatcattgtcatagaaagtgttaaagccat
acatccaactttgttggaaggtacgcaccccagcgccgatattaagggtattgcgactgtctttatttcttatacccaa
ctgactaaaaaagacatatgaagcagagtcataccaaggcaacagccaatcaagagaactttctttgagggagaacttt
ttatcaaagttaacattgacctgagttgtcccaaatctatttaaccagtgttttacttcctgatttgccgcgtcattta
ccattgacctggtgatattggatgcattacccgataccgccagactggttccggtactgattatcttattgaaagcctc
attatgttcagcggggttctctaacgcttctgaattaaaataatgttgctgtgagaacccataagtaaatattccaaat
attaaaccgatcgataatattagcctgctaattattttagtcacagttagcgtattaaaaaatgaatacattactagag
tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggt
gatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaattta
tttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgag
atacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatattt
ttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaa
aaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacat
catggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaacta
gcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaat
ctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacaca
tggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttata