BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961224 1 -35 range1961224 1 137 142 annotation1961225 1 -10 range1961225 1 161 166 annotation1961227 1 start range1961227 1 173 173 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961226 1 LacI binding site range1961226 1 166 200 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 annotation7027 1 BBa_B0032 range7027 1 1 13 BBa_K1677011 1 BBa_K1677011 IPTG inducible invasin with a GFP reporter 2015-07-25T11:00:00Z 2015-07-26T07:44:11Z Invasin parts from Warsaw iGEM Team from registry, IPTG promoter and GFP is from the registry. The invasin gene and a GFP reporter gene under the control of an IPTG inducible promoter. false false _2095_ 25020 25020 9 false -- false Manan Shah component2433499 1 BBa_E0240 component2433488 1 BBa_K299810 component2433482 1 BBa_J04500 annotation2433482 1 BBa_J04500 range2433482 1 1 220 annotation2433488 1 BBa_K299810 range2433488 1 229 2757 annotation2433499 1 BBa_E0240 range2433499 1 2766 3641 BBa_K299810 1 BBa_K299810 B0032+Invasin 2010-10-12T11:00:00Z 2015-05-08T01:11:50Z false false _419_ 0 7677 9 It's complicated true false Micha&#322; Lower component2085755 1 BBa_B0032 component2085758 1 BBa_K177010 annotation2085758 1 BBa_K177010 range2085758 1 22 2529 annotation2085755 1 BBa_B0032 range2085755 1 1 13 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K177010 1 BBa_K177010 invasine locus from yersinia 2009-07-06T11:00:00Z 2015-05-08T01:11:05Z Genomic DNA from Yersinia enable bacteria to enter a cell false false _284_ 0 4696 9 Not in stock false none false Kamila Ornoch annotation2010434 1 protein range2010434 1 1 2826 BBa_E0240 1 GFP report GFP generator 2004-10-17T11:00:00Z 2015-08-31T04:07:26Z Released HQ 2013 B0032.E0040.B0015 false true _11_1_ 0 61 7 In stock false true Jennifer Braff component1249216 1 BBa_E0040 component1249221 1 BBa_B0010 component1249213 1 BBa_B0032 component1249231 1 BBa_B0012 annotation1249231 1 BBa_B0012 range1249231 1 836 876 annotation1249213 1 BBa_B0032 range1249213 1 1 13 annotation1249221 1 BBa_B0010 range1249221 1 748 827 annotation1249216 1 BBa_E0040 range1249216 1 20 739 BBa_J04500 1 BBa_J04500 IPTG inducible promoter with RBS 2005-06-08T11:00:00Z 2015-08-31T04:08:14Z Davidson Synth-Aces Released HQ 2013 R0010.B0034 false true _16_ 0 326 16 In stock false false Kristen DeCelle component1508149 1 BBa_R0010 component1508159 1 BBa_B0034 annotation1508149 1 BBa_R0010 range1508149 1 1 200 annotation1508159 1 BBa_B0034 range1508159 1 209 220 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K299810_sequence 1 tcacacaggaaagtactagagctattgaggctccgcacacagcggatacgccgtagccgcagatgttggtatgtcgccggtggtcatatccatagtgacaaaatctgtactggtctttttagtccaatagttacccgatggccactcagcgctatcataggttgccaaacttccccactctccccacagagtcccgtctggtgaacgcgaaccattactggcgcgtgcggactcgatcagcgcagtaaaatcagtgcgctggcaatttcgactcgcctcgacacttgattgcagtgacatggtgccggagaacacccacaaattaggtttgaattgatatgttgccgtataactcgggaattttttactttttgccgtcacagtgacggtgctaccataggttttataggcaatattgactttaccctgattatcaaccggcgcactggccgcataggatgatgtccagtcatattgagtattattcgccacatcgtcattcatcaccaattggaacgtggctttattaaaggtagttttcgggaagcctttatctgtggcaaattgctcaccattcaccttaatgccggttatcttcggtactgggtacagggtgattcttttctgcaacaagtctagtgattccccccccacctgcggcgtaatatcgacatctcctacggaatttcccaccacactggcggtatagttgtcagcattttcggttaccgaactaatagttaccggaacaccactttgtataaattcaagatctgttatcccactgacaaactcattatttttattacgcggaacaaaggtgagtatcgactgcatggagccatcggcaacaatatcagatggagaaacgttaaaacttgagtttgtgacatctggcggcagcacggcaaatcgtacatcaacagtagcctgttgcccctgaatatctaaagtgacgactgacaccccaacagtgaagctagttaatgcaatgcttatcacaccctgtgcatcggttttttttgtgactttactgggaagcgcaccattactggtggttatcaccccctcttgctcggcaaccggcgtactatcaatattagtcactgtaaatttcaccgtgatgggagtcctgccatcagcaagcgcaccatcatcagtcacttccgaggtaataacgaactgaggttgctgcacaataacggtcaacgtagctggattagaactattgccgtggttatcgatagccgtggcgctgagggtataggtattgaccgccgcttcgctctcgccggttttccccacagtacgactcgctggtgccatcggtttataaggcggtaagaccacattgtaatctgttgcactgacgggtattaatttgccgccggcagcaacccattgcgcatcattccagagaatacgttgtagtgcagattgagactgtatttgtgcactaacggaataaacctgccccggcaggccggagagtacggcgggtgaaaatgccaatttgatagtattctgtttttggtattccagaacaatatttggattgcgctcaaccagattataacggctctcagccagtaaacgagttccggccaccactgcggggctaaactgcgaacgaaaactttcgccgaggcgataatccatttgtaagttccattgtatttcatgctgccgactttttcccattcgttgatcgacccccagtgtaatgaaggggatcggcgtataaataagccctgtggtcaccgcataagggttactttgcaggttatctttaccaaataaagccacccgctcaccacggtactgctcatattttaatttcccgcccaattgtggcagcgcaggtaaatacgctttgacgtgaatgtcgcccccgcttgccgggcgctcattatagtccgcgaaatcacgagattgatgccaaccattgaggcgaaaataaccattggccgataattgtaaataatcagtccaggcttctgcacccacgccaatacgatgattgtgcccggtcatatcattgtcatagaaagtgttaaagccatacatccaactttgttggaaggtacgcaccccagcgccgatattaagggtattgcgactgtctttatttcttatacccaactgactaaaaaagacatatgaagcagagtcataccaaggcaacagccaatcaagagaactttctttgagggagaactttttatcaaagttaacattgacctgagttgtcccaaatctatttaaccagtgttttacttcctgatttgccgcgtcatttaccattgacctggtgatattggatgcattacccgataccgccagactggttccggtactgattatcttattgaaagcctcattatgttcagcggggttctctaacgcttctgaattaaaataatgttgctgtgagaacccataagtaaatattccaaatattaaaccgatcgataatattagcctgctaattattttagtcacagttagcgtattaaaaaatgaatacat BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1677011_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagtcacacaggaaagtactagagctattgaggctccgcacacagcggatacgccgtagccgcagatgttggtatgtcgccggtggtcatatccatagtgacaaaatctgtactggtctttttagtccaatagttacccgatggccactcagcgctatcataggttgccaaacttccccactctccccacagagtcccgtctggtgaacgcgaaccattactggcgcgtgcggactcgatcagcgcagtaaaatcagtgcgctggcaatttcgactcgcctcgacacttgattgcagtgacatggtgccggagaacacccacaaattaggtttgaattgatatgttgccgtataactcgggaattttttactttttgccgtcacagtgacggtgctaccataggttttataggcaatattgactttaccctgattatcaaccggcgcactggccgcataggatgatgtccagtcatattgagtattattcgccacatcgtcattcatcaccaattggaacgtggctttattaaaggtagttttcgggaagcctttatctgtggcaaattgctcaccattcaccttaatgccggttatcttcggtactgggtacagggtgattcttttctgcaacaagtctagtgattccccccccacctgcggcgtaatatcgacatctcctacggaatttcccaccacactggcggtatagttgtcagcattttcggttaccgaactaatagttaccggaacaccactttgtataaattcaagatctgttatcccactgacaaactcattatttttattacgcggaacaaaggtgagtatcgactgcatggagccatcggcaacaatatcagatggagaaacgttaaaacttgagtttgtgacatctggcggcagcacggcaaatcgtacatcaacagtagcctgttgcccctgaatatctaaagtgacgactgacaccccaacagtgaagctagttaatgcaatgcttatcacaccctgtgcatcggttttttttgtgactttactgggaagcgcaccattactggtggttatcaccccctcttgctcggcaaccggcgtactatcaatattagtcactgtaaatttcaccgtgatgggagtcctgccatcagcaagcgcaccatcatcagtcacttccgaggtaataacgaactgaggttgctgcacaataacggtcaacgtagctggattagaactattgccgtggttatcgatagccgtggcgctgagggtataggtattgaccgccgcttcgctctcgccggttttccccacagtacgactcgctggtgccatcggtttataaggcggtaagaccacattgtaatctgttgcactgacgggtattaatttgccgccggcagcaacccattgcgcatcattccagagaatacgttgtagtgcagattgagactgtatttgtgcactaacggaataaacctgccccggcaggccggagagtacggcgggtgaaaatgccaatttgatagtattctgtttttggtattccagaacaatatttggattgcgctcaaccagattataacggctctcagccagtaaacgagttccggccaccactgcggggctaaactgcgaacgaaaactttcgccgaggcgataatccatttgtaagttccattgtatttcatgctgccgactttttcccattcgttgatcgacccccagtgtaatgaaggggatcggcgtataaataagccctgtggtcaccgcataagggttactttgcaggttatctttaccaaataaagccacccgctcaccacggtactgctcatattttaatttcccgcccaattgtggcagcgcaggtaaatacgctttgacgtgaatgtcgcccccgcttgccgggcgctcattatagtccgcgaaatcacgagattgatgccaaccattgaggcgaaaataaccattggccgataattgtaaataatcagtccaggcttctgcacccacgccaatacgatgattgtgcccggtcatatcattgtcatagaaagtgttaaagccatacatccaactttgttggaaggtacgcaccccagcgccgatattaagggtattgcgactgtctttatttcttatacccaactgactaaaaaagacatatgaagcagagtcataccaaggcaacagccaatcaagagaactttctttgagggagaactttttatcaaagttaacattgacctgagttgtcccaaatctatttaaccagtgttttacttcctgatttgccgcgtcatttaccattgacctggtgatattggatgcattacccgataccgccagactggttccggtactgattatcttattgaaagcctcattatgttcagcggggttctctaacgcttctgaattaaaataatgttgctgtgagaacccataagtaaatattccaaatattaaaccgatcgataatattagcctgctaattattttagtcacagttagcgtattaaaaaatgaatacattactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_J04500_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa BBa_B0032_sequence 1 tcacacaggaaag BBa_E0240_sequence 1 tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K177010_sequence 1 ctattgaggctccgcacacagcggatacgccgtagccgcagatgttggtatgtcgccggtggtcatatccatagtgacaaaatctgtactggtctttttagtccaatagttacccgatggccactcagcgctatcataggttgccaaacttccccactctccccacagagtcccgtctggtgaacgcgaaccattactggcgcgtgcggactcgatcagcgcagtaaaatcagtgcgctggcaatttcgactcgcctcgacacttgattgcagtgacatggtgccggagaacacccacaaattaggtttgaattgatatgttgccgtataactcgggaattttttactttttgccgtcacagtgacggtgctaccataggttttataggcaatattgactttaccctgattatcaaccggcgcactggccgcataggatgatgtccagtcatattgagtattattcgccacatcgtcattcatcaccaattggaacgtggctttattaaaggtagttttcgggaagcctttatctgtggcaaattgctcaccattcaccttaatgccggttatcttcggtactgggtacagggtgattcttttctgcaacaagtctagtgattccccccccacctgcggcgtaatatcgacatctcctacggaatttcccaccacactggcggtatagttgtcagcattttcggttaccgaactaatagttaccggaacaccactttgtataaattcaagatctgttatcccactgacaaactcattatttttattacgcggaacaaaggtgagtatcgactgcatggagccatcggcaacaatatcagatggagaaacgttaaaacttgagtttgtgacatctggcggcagcacggcaaatcgtacatcaacagtagcctgttgcccctgaatatctaaagtgacgactgacaccccaacagtgaagctagttaatgcaatgcttatcacaccctgtgcatcggttttttttgtgactttactgggaagcgcaccattactggtggttatcaccccctcttgctcggcaaccggcgtactatcaatattagtcactgtaaatttcaccgtgatgggagtcctgccatcagcaagcgcaccatcatcagtcacttccgaggtaataacgaactgaggttgctgcacaataacggtcaacgtagctggattagaactattgccgtggttatcgatagccgtggcgctgagggtataggtattgaccgccgcttcgctctcgccggttttccccacagtacgactcgctggtgccatcggtttataaggcggtaagaccacattgtaatctgttgcactgacgggtattaatttgccgccggcagcaacccattgcgcatcattccagagaatacgttgtagtgcagattgagactgtatttgtgcactaacggaataaacctgccccggcaggccggagagtacggcgggtgaaaatgccaatttgatagtattctgtttttggtattccagaacaatatttggattgcgctcaaccagattataacggctctcagccagtaaacgagttccggccaccactgcggggctaaactgcgaacgaaaactttcgccgaggcgataatccatttgtaagttccattgtatttcatgctgccgactttttcccattcgttgatcgacccccagtgtaatgaaggggatcggcgtataaataagccctgtggtcaccgcataagggttactttgcaggttatctttaccaaataaagccacccgctcaccacggtactgctcatattttaatttcccgcccaattgtggcagcgcaggtaaatacgctttgacgtgaatgtcgcccccgcttgccgggcgctcattatagtccgcgaaatcacgagattgatgccaaccattgaggcgaaaataaccattggccgataattgtaaataatcagtccaggcttctgcacccacgccaatacgatgattgtgcccggtcatatcattgtcatagaaagtgttaaagccatacatccaactttgttggaaggtacgcaccccagcgccgatattaagggtattgcgactgtctttatttcttatacccaactgactaaaaaagacatatgaagcagagtcataccaaggcaacagccaatcaagagaactttctttgagggagaactttttatcaaagttaacattgacctgagttgtcccaaatctatttaaccagtgttttacttcctgatttgccgcgtcatttaccattgacctggtgatattggatgcattacccgataccgccagactggttccggtactgattatcttattgaaagcctcattatgttcagcggggttctctaacgcttctgaattaaaataatgttgctgtgagaacccataagtaaatattccaaatattaaaccgatcgataatattagcctgctaattattttagtcacagttagcgtattaaaaaatgaatacat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z