LOCUS       BBa_K1677012            1837 bp    DNA     linear       17-May-2024
DEFINITION  IPTG inducible listeriolysin
ACCESSION   BBa_K1677012
VERSION     BBa_K1677012.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     RBS             229..241
                     /label=RBS-3\Weak
     RBS             235..238
                     /label=RBS
     misc_feature    229..241
                     /label=BBa_B0032
     RBS             229..241
                     /label=BBa_B0032
     CDS             248..1837
                     /label=protein
     CDS             248..1837
                     /label=BBa_K177026
     misc_feature    229..1837
                     /label=BBa_K299811
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagtc acacaggaaa
      241 gtactagatg aaaaaaataa tgctagtttt tattacactt atattagtta gtctaccaat
      301 tgcgcaacaa actgaagcaa aggatgcatc tgcattcaat aaagaaaatt taatttcatc
      361 catagcacca ccagcatctc cgcctgcaag tcctaagacg ccaatcgaaa agaaacacgc
      421 ggatgaaatc gataagtata tacaaggatt ggattacaat aaaaacaatg tattagtata
      481 ccacggagat gcagtgacaa atgtgccgcc aagaaaaggt tataaagatg gaaatgaata
      541 tatcgttgtg gagaaaaaga agaaatccat caatcaaaat aatgcagata tccaagttgt
      601 gaatgcaatt tcgagcctaa catatccagg tgctctcgtg aaagccaatt cggaattagt
      661 agaaaatcaa cccgatgttc ttcctgtcaa acgtgattca ttaacactta gcattgattt
      721 gccaggaatg actaatcaag acaataaaat tgttgtaaaa aatgctacta aatcgaacgt
      781 taacaacgca gtaaatacat tagtggaaag atggaatgaa aaatatgctc aagcttatcc
      841 aaatgtaagt gcaaaaattg attatgatga cgaaatggct tacagtgaat cacaattaat
      901 tgcaaaattt ggtacggcat ttaaagctgt aaataatagc ttgaatgtaa acttcggcgc
      961 aatcagtgaa gggaaaatgc aagaagaagt cattagtttt aaacaaattt actataacgt
     1021 gaatgttaat gaacctacaa gaccttccag atttttcggc aaagctgtta ctaaagagca
     1081 gttgcaagcg cttggagtga atgcagaaaa tcctcctgca tatatctcaa gtgtggcata
     1141 tggccgtcaa gtttatttga aattatcaac taattcccat agtaccaaag taaaagctgc
     1201 ttttgacgct gccgtaagtg ggaaatctgt ctcaggtgat gtagaactga caaatatcat
     1261 caaaaattct tccttcaaag ccgtaattta cggtggctcc gcaaaagatg aagttcaaat
     1321 catcgacggt aacctcggag acttacgaga tattttgaaa aaaggtgcta cttttaaccg
     1381 ggaaacacca ggagttccca ttgcctatac aacaaacttc ttaaaagaca atgaattagc
     1441 tgttattaaa aacaactcag aatatattga aacaacttca aaagcttata cagatggaaa
     1501 aatcaacatc gatcactctg gaggatacgt tgctcaattc aacatctctt gggatgaaat
     1561 aaattatgat cctgaaggta acgaaattgt tcaacataaa aactggagcg aaaacaataa
     1621 aagtaagcta gctcatttca catcgtccat ctatttgcca ggtaacgcaa gaaatattaa
     1681 tgtttacgct aaagaatgca ctggtttagc ttgggaatgg tggagaacgg taattgatga
     1741 ccggaaccta ccgcttgtga aaaatagaaa tatctccatc tggggcacta cactttatcc
     1801 gaaatatagt aatagtgtag ataatccaat cgaataa
//