LOCUS       BBa_K1677013            2721 bp    DNA     linear       17-May-2024
DEFINITION  IPTG inducible listeriolysin with a GFP reporter
ACCESSION   BBa_K1677013
VERSION     BBa_K1677013.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     RBS             229..241
                     /label=RBS-3\Weak
     RBS             235..238
                     /label=RBS
     misc_feature    229..241
                     /label=BBa_B0032
     RBS             229..241
                     /label=BBa_B0032
     CDS             248..1837
                     /label=protein
     CDS             248..1837
                     /label=BBa_K177026
     misc_feature    229..1837
                     /label=BBa_K299811
     RBS             1846..1858
                     /label=RBS-3\Weak
     RBS             1852..1855
                     /label=RBS
     misc_feature    1846..1858
                     /label=BBa_B0032
     RBS             1846..1858
                     /label=BBa_B0032
     CDS             1865..2584
                     /label=GFP protein
     CDS             1865..2584
                     /label=BBa_E0040
     stem_loop       2604..2647
                     /label=stem_loop
     misc_feature    2593..2672
                     /label=BBa_B0010
     terminator      2593..2672
                     /label=BBa_B0010
     stem_loop       2688..2707
                     /label=T7 TE
     misc_feature    2714..2714
                     /label=stop
     polyA_site      2708..2721
                     /label=polya
     misc_feature    2681..2721
                     /label=BBa_B0012
     terminator      2681..2721
                     /label=BBa_B0012
     misc_feature    1846..2721
                     /label=BBa_E0240
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagtc acacaggaaa
      241 gtactagatg aaaaaaataa tgctagtttt tattacactt atattagtta gtctaccaat
      301 tgcgcaacaa actgaagcaa aggatgcatc tgcattcaat aaagaaaatt taatttcatc
      361 catagcacca ccagcatctc cgcctgcaag tcctaagacg ccaatcgaaa agaaacacgc
      421 ggatgaaatc gataagtata tacaaggatt ggattacaat aaaaacaatg tattagtata
      481 ccacggagat gcagtgacaa atgtgccgcc aagaaaaggt tataaagatg gaaatgaata
      541 tatcgttgtg gagaaaaaga agaaatccat caatcaaaat aatgcagata tccaagttgt
      601 gaatgcaatt tcgagcctaa catatccagg tgctctcgtg aaagccaatt cggaattagt
      661 agaaaatcaa cccgatgttc ttcctgtcaa acgtgattca ttaacactta gcattgattt
      721 gccaggaatg actaatcaag acaataaaat tgttgtaaaa aatgctacta aatcgaacgt
      781 taacaacgca gtaaatacat tagtggaaag atggaatgaa aaatatgctc aagcttatcc
      841 aaatgtaagt gcaaaaattg attatgatga cgaaatggct tacagtgaat cacaattaat
      901 tgcaaaattt ggtacggcat ttaaagctgt aaataatagc ttgaatgtaa acttcggcgc
      961 aatcagtgaa gggaaaatgc aagaagaagt cattagtttt aaacaaattt actataacgt
     1021 gaatgttaat gaacctacaa gaccttccag atttttcggc aaagctgtta ctaaagagca
     1081 gttgcaagcg cttggagtga atgcagaaaa tcctcctgca tatatctcaa gtgtggcata
     1141 tggccgtcaa gtttatttga aattatcaac taattcccat agtaccaaag taaaagctgc
     1201 ttttgacgct gccgtaagtg ggaaatctgt ctcaggtgat gtagaactga caaatatcat
     1261 caaaaattct tccttcaaag ccgtaattta cggtggctcc gcaaaagatg aagttcaaat
     1321 catcgacggt aacctcggag acttacgaga tattttgaaa aaaggtgcta cttttaaccg
     1381 ggaaacacca ggagttccca ttgcctatac aacaaacttc ttaaaagaca atgaattagc
     1441 tgttattaaa aacaactcag aatatattga aacaacttca aaagcttata cagatggaaa
     1501 aatcaacatc gatcactctg gaggatacgt tgctcaattc aacatctctt gggatgaaat
     1561 aaattatgat cctgaaggta acgaaattgt tcaacataaa aactggagcg aaaacaataa
     1621 aagtaagcta gctcatttca catcgtccat ctatttgcca ggtaacgcaa gaaatattaa
     1681 tgtttacgct aaagaatgca ctggtttagc ttgggaatgg tggagaacgg taattgatga
     1741 ccggaaccta ccgcttgtga aaaatagaaa tatctccatc tggggcacta cactttatcc
     1801 gaaatatagt aatagtgtag ataatccaat cgaataatac tagagtcaca caggaaagta
     1861 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
     1921 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
     1981 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
     2041 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
     2101 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
     2161 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
     2221 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
     2281 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
     2341 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
     2401 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     2461 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     2521 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
     2581 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2641 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     2701 gtgggccttt ctgcgtttat a
//